Screening for the anti-inflammation quality markers of Xiaojin Pills based on HPLC-MS/MS method, COX-2 inhibition test and protein interaction network

Abstract Nowadays, breast disorders seriously affect women’s health in an increasing number. In China, Xiaojin Pills are commonly used in the treatment of breast diseases. Doctors have concluded that the combined use of Xiaojin Pills with conventional therapy can significantly improve the efficacy w...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xi Xiong, Ya-nan He, Bi Feng, Yuan Pan, Hai-zhu Zhang, Xiu-mei Ke, Yi Zhang, Ming Yang, Li Han, Ding-kun Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4f3db0f7583945759d907e0e00e044e6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4f3db0f7583945759d907e0e00e044e6
record_format	dspace
spelling	oai:doaj.org-article:4f3db0f7583945759d907e0e00e044e62021-12-02T11:40:34ZScreening for the anti-inflammation quality markers of Xiaojin Pills based on HPLC-MS/MS method, COX-2 inhibition test and protein interaction network10.1038/s41598-018-25582-72045-2322https://doaj.org/article/4f3db0f7583945759d907e0e00e044e62018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25582-7https://doaj.org/toc/2045-2322Abstract Nowadays, breast disorders seriously affect women’s health in an increasing number. In China, Xiaojin Pills are commonly used in the treatment of breast diseases. Doctors have concluded that the combined use of Xiaojin Pills with conventional therapy can significantly improve the efficacy with fewer side effects. However, the prescription of Xiaojin Pills is complicated and their quality control methods cannot completely ensure the quality of Xiaojin Pills. On the basis of its mechanism, our study combined chemical evaluation and biological evaluation to identify the anti-inflammatory markers of Xiaojin Pills. In this manuscript, 13 compounds in Xiaojin Pills were quantified. At the same time, the cyclooxygenase-2 inhibition rates of different Xiaojin Pills were measured and the possible markers were screened by spectrum-effect relationship. Further, anti-inflammatory activities of markers were verified and protein interaction network was analyzed, identifying the components of Protocatechuate, Beta-Boswellic acid and Levistilide A as the anti-inflammatory quality markers of Xiaojin Pills. We hope our studies can provide a scientific theoretical basis for accurately quality control of Xiaojin Pills and reasonable suggestions for pharmaceutical companies and new ideas for the quality control of other medicines.Xi XiongYa-nan HeBi FengYuan PanHai-zhu ZhangXiu-mei KeYi ZhangMing YangLi HanDing-kun ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xi Xiong Ya-nan He Bi Feng Yuan Pan Hai-zhu Zhang Xiu-mei Ke Yi Zhang Ming Yang Li Han Ding-kun Zhang Screening for the anti-inflammation quality markers of Xiaojin Pills based on HPLC-MS/MS method, COX-2 inhibition test and protein interaction network
description	Abstract Nowadays, breast disorders seriously affect women’s health in an increasing number. In China, Xiaojin Pills are commonly used in the treatment of breast diseases. Doctors have concluded that the combined use of Xiaojin Pills with conventional therapy can significantly improve the efficacy with fewer side effects. However, the prescription of Xiaojin Pills is complicated and their quality control methods cannot completely ensure the quality of Xiaojin Pills. On the basis of its mechanism, our study combined chemical evaluation and biological evaluation to identify the anti-inflammatory markers of Xiaojin Pills. In this manuscript, 13 compounds in Xiaojin Pills were quantified. At the same time, the cyclooxygenase-2 inhibition rates of different Xiaojin Pills were measured and the possible markers were screened by spectrum-effect relationship. Further, anti-inflammatory activities of markers were verified and protein interaction network was analyzed, identifying the components of Protocatechuate, Beta-Boswellic acid and Levistilide A as the anti-inflammatory quality markers of Xiaojin Pills. We hope our studies can provide a scientific theoretical basis for accurately quality control of Xiaojin Pills and reasonable suggestions for pharmaceutical companies and new ideas for the quality control of other medicines.
format	article
author	Xi Xiong Ya-nan He Bi Feng Yuan Pan Hai-zhu Zhang Xiu-mei Ke Yi Zhang Ming Yang Li Han Ding-kun Zhang
author_facet	Xi Xiong Ya-nan He Bi Feng Yuan Pan Hai-zhu Zhang Xiu-mei Ke Yi Zhang Ming Yang Li Han Ding-kun Zhang
author_sort	Xi Xiong
title	Screening for the anti-inflammation quality markers of Xiaojin Pills based on HPLC-MS/MS method, COX-2 inhibition test and protein interaction network
title_short	Screening for the anti-inflammation quality markers of Xiaojin Pills based on HPLC-MS/MS method, COX-2 inhibition test and protein interaction network
title_full	Screening for the anti-inflammation quality markers of Xiaojin Pills based on HPLC-MS/MS method, COX-2 inhibition test and protein interaction network
title_fullStr	Screening for the anti-inflammation quality markers of Xiaojin Pills based on HPLC-MS/MS method, COX-2 inhibition test and protein interaction network
title_full_unstemmed	Screening for the anti-inflammation quality markers of Xiaojin Pills based on HPLC-MS/MS method, COX-2 inhibition test and protein interaction network
title_sort	screening for the anti-inflammation quality markers of xiaojin pills based on hplc-ms/ms method, cox-2 inhibition test and protein interaction network
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/4f3db0f7583945759d907e0e00e044e6
work_keys_str_mv	AT xixiong screeningfortheantiinflammationqualitymarkersofxiaojinpillsbasedonhplcmsmsmethodcox2inhibitiontestandproteininteractionnetwork AT yananhe screeningfortheantiinflammationqualitymarkersofxiaojinpillsbasedonhplcmsmsmethodcox2inhibitiontestandproteininteractionnetwork AT bifeng screeningfortheantiinflammationqualitymarkersofxiaojinpillsbasedonhplcmsmsmethodcox2inhibitiontestandproteininteractionnetwork AT yuanpan screeningfortheantiinflammationqualitymarkersofxiaojinpillsbasedonhplcmsmsmethodcox2inhibitiontestandproteininteractionnetwork AT haizhuzhang screeningfortheantiinflammationqualitymarkersofxiaojinpillsbasedonhplcmsmsmethodcox2inhibitiontestandproteininteractionnetwork AT xiumeike screeningfortheantiinflammationqualitymarkersofxiaojinpillsbasedonhplcmsmsmethodcox2inhibitiontestandproteininteractionnetwork AT yizhang screeningfortheantiinflammationqualitymarkersofxiaojinpillsbasedonhplcmsmsmethodcox2inhibitiontestandproteininteractionnetwork AT mingyang screeningfortheantiinflammationqualitymarkersofxiaojinpillsbasedonhplcmsmsmethodcox2inhibitiontestandproteininteractionnetwork AT lihan screeningfortheantiinflammationqualitymarkersofxiaojinpillsbasedonhplcmsmsmethodcox2inhibitiontestandproteininteractionnetwork AT dingkunzhang screeningfortheantiinflammationqualitymarkersofxiaojinpillsbasedonhplcmsmsmethodcox2inhibitiontestandproteininteractionnetwork
_version_	1718395580408922112

Screening for the anti-inflammation quality markers of Xiaojin Pills based on HPLC-MS/MS method, COX-2 inhibition test and protein interaction network

Ejemplares similares