Metabolic Profiles, Bioactive Compounds, and Antioxidant Capacity in Lentinula edodes Cultivated on Log versus Sawdust Substrates

Lentinula edodes (shiitake) is a popular nutritious edible mushroom with a desirable aroma and flavor. Traditional cultivation of L. edodes on beds of logs has been replaced by cultivation on sawdust, but the effects of cultivation changes on L. edodes&...

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Autores principales:	Miso Nam, Ji Yeon Choi, Min-Sun Kim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Lentinula edodes</i> metabolite profiling bioactive compounds antioxidant capacity log bed cultivation sawdust media cultivation Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4f5285bff7314f619679115beee3afee
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spelling	oai:doaj.org-article:4f5285bff7314f619679115beee3afee2021-11-25T16:53:26ZMetabolic Profiles, Bioactive Compounds, and Antioxidant Capacity in <i>Lentinula edodes</i> Cultivated on Log versus Sawdust Substrates10.3390/biom111116542218-273Xhttps://doaj.org/article/4f5285bff7314f619679115beee3afee2021-11-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1654https://doaj.org/toc/2218-273X<i>Lentinula edodes</i> (shiitake) is a popular nutritious edible mushroom with a desirable aroma and flavor. Traditional cultivation of <i>L. edodes</i> on beds of logs has been replaced by cultivation on sawdust, but the effects of cultivation changes on <i>L. edodes</i> mushrooms have not been well characterized. We determined the metabolic profile, bioactive compounds, and antioxidant capacity in <i>L. edodes</i> grown on log or sawdust substrates. Metabolic profiles of <i>L. edodes</i> extracts were determined by <sup>1</sup>H nuclear magnetic resonance (NMR) and ultra-performance liquid chromatography to quadrupole time-of-flight mass spectrometry. Principal component analysis score plots from <sup>1</sup>H NMR analysis showed clear differences between samples. Concentrations of primary metabolites, especially amino acids, generally decreased in <i>L. edodes</i> grown on logs compared to sawdust. Phenolic compounds showed variations in concentration depending on the cultivation method. Bioactive compounds and their antioxidant capacity were analyzed spectrophotometrically. <i>L. edodes</i> cultivated on logs had high concentrations of bioactive compounds with strong antioxidant capacity compared to <i>L. edodes</i> cultivated on sawdust. Thus, the concentration of primary metabolites was high in <i>L. edodes</i> grown on sawdust, which produces a high growth rate. In contrast, log-cultivated <i>L. edodes</i>, which were similar to wild mushrooms, had high levels of bioactive compounds and high antioxidant capacity. This information is useful for determining optimal cultivation conditions for nutritional and medicinal uses of <i>L. edodes</i> mushrooms.Miso NamJi Yeon ChoiMin-Sun KimMDPI AGarticle<i>Lentinula edodes</i>metabolite profilingbioactive compoundsantioxidant capacitylog bed cultivationsawdust media cultivationMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1654, p 1654 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Lentinula edodes</i> metabolite profiling bioactive compounds antioxidant capacity log bed cultivation sawdust media cultivation Microbiology QR1-502
spellingShingle	<i>Lentinula edodes</i> metabolite profiling bioactive compounds antioxidant capacity log bed cultivation sawdust media cultivation Microbiology QR1-502 Miso Nam Ji Yeon Choi Min-Sun Kim Metabolic Profiles, Bioactive Compounds, and Antioxidant Capacity in <i>Lentinula edodes</i> Cultivated on Log versus Sawdust Substrates
description	<i>Lentinula edodes</i> (shiitake) is a popular nutritious edible mushroom with a desirable aroma and flavor. Traditional cultivation of <i>L. edodes</i> on beds of logs has been replaced by cultivation on sawdust, but the effects of cultivation changes on <i>L. edodes</i> mushrooms have not been well characterized. We determined the metabolic profile, bioactive compounds, and antioxidant capacity in <i>L. edodes</i> grown on log or sawdust substrates. Metabolic profiles of <i>L. edodes</i> extracts were determined by <sup>1</sup>H nuclear magnetic resonance (NMR) and ultra-performance liquid chromatography to quadrupole time-of-flight mass spectrometry. Principal component analysis score plots from <sup>1</sup>H NMR analysis showed clear differences between samples. Concentrations of primary metabolites, especially amino acids, generally decreased in <i>L. edodes</i> grown on logs compared to sawdust. Phenolic compounds showed variations in concentration depending on the cultivation method. Bioactive compounds and their antioxidant capacity were analyzed spectrophotometrically. <i>L. edodes</i> cultivated on logs had high concentrations of bioactive compounds with strong antioxidant capacity compared to <i>L. edodes</i> cultivated on sawdust. Thus, the concentration of primary metabolites was high in <i>L. edodes</i> grown on sawdust, which produces a high growth rate. In contrast, log-cultivated <i>L. edodes</i>, which were similar to wild mushrooms, had high levels of bioactive compounds and high antioxidant capacity. This information is useful for determining optimal cultivation conditions for nutritional and medicinal uses of <i>L. edodes</i> mushrooms.
format	article
author	Miso Nam Ji Yeon Choi Min-Sun Kim
author_facet	Miso Nam Ji Yeon Choi Min-Sun Kim
author_sort	Miso Nam
title	Metabolic Profiles, Bioactive Compounds, and Antioxidant Capacity in <i>Lentinula edodes</i> Cultivated on Log versus Sawdust Substrates
title_short	Metabolic Profiles, Bioactive Compounds, and Antioxidant Capacity in <i>Lentinula edodes</i> Cultivated on Log versus Sawdust Substrates
title_full	Metabolic Profiles, Bioactive Compounds, and Antioxidant Capacity in <i>Lentinula edodes</i> Cultivated on Log versus Sawdust Substrates
title_fullStr	Metabolic Profiles, Bioactive Compounds, and Antioxidant Capacity in <i>Lentinula edodes</i> Cultivated on Log versus Sawdust Substrates
title_full_unstemmed	Metabolic Profiles, Bioactive Compounds, and Antioxidant Capacity in <i>Lentinula edodes</i> Cultivated on Log versus Sawdust Substrates
title_sort	metabolic profiles, bioactive compounds, and antioxidant capacity in <i>lentinula edodes</i> cultivated on log versus sawdust substrates
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4f5285bff7314f619679115beee3afee
work_keys_str_mv	AT misonam metabolicprofilesbioactivecompoundsandantioxidantcapacityinilentinulaedodesicultivatedonlogversussawdustsubstrates AT jiyeonchoi metabolicprofilesbioactivecompoundsandantioxidantcapacityinilentinulaedodesicultivatedonlogversussawdustsubstrates AT minsunkim metabolicprofilesbioactivecompoundsandantioxidantcapacityinilentinulaedodesicultivatedonlogversussawdustsubstrates
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Metabolic Profiles, Bioactive Compounds, and Antioxidant Capacity in <i>Lentinula edodes</i> Cultivated on Log versus Sawdust Substrates

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