Ultrasound-Based Method for the Identification of Novel MicroRNA Biomarkers in Prostate Cancer

The detection of circulating microRNA (miRNA)-based biomarkers represents an innovative, non-invasive method for the early detection of cancer. However, the low concentration of miRNAs released in body fluids and the difficult identification of the tumor site have limited their clinical use as effec...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jessica Cornice, Daria Capece, Mauro Di Vito Nolfi, Monica Di Padova, Chiara Compagnoni, Daniela Verzella, Barbara Di Francesco, Davide Vecchiotti, Irene Flati, Alessandra Tessitore, Edoardo Alesse, Gaetano Barbato, Francesca Zazzeroni
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	miRNAs biomarkers prostate cancer ultrasounds Genetics QH426-470
Acceso en línea:	https://doaj.org/article/4f632102159d48e6b95e131f44d658ec
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4f632102159d48e6b95e131f44d658ec
record_format	dspace
spelling	oai:doaj.org-article:4f632102159d48e6b95e131f44d658ec2021-11-25T17:41:17ZUltrasound-Based Method for the Identification of Novel MicroRNA Biomarkers in Prostate Cancer10.3390/genes121117262073-4425https://doaj.org/article/4f632102159d48e6b95e131f44d658ec2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1726https://doaj.org/toc/2073-4425The detection of circulating microRNA (miRNA)-based biomarkers represents an innovative, non-invasive method for the early detection of cancer. However, the low concentration of miRNAs released in body fluids and the difficult identification of the tumor site have limited their clinical use as effective cancer biomarkers. To evaluate if ultrasound treatment could amplify the release of extracellular cancer biomarkers, we treated a panel of prostate cancer (PCa) cell lines with an ultrasound-based prototype and profiled the release of miRNAs in the extracellular space, with the aim of identifying novel miRNA-based biomarkers that could be used for PCa diagnosis and the monitoring of tumor evolution. We provide evidence that US-mediated sonoporation amplifies the release of miRNAs from both androgen-dependent (AD) and -independent (AI) PCa cells. We identified four PCa-related miRNAs, whose levels in LNCaP and DU145 supernatants were significantly increased following ultrasound treatment: mir-629-5p, mir-374-5p, mir-194-5p, and let-7d-5p. We further analyzed a publicly available dataset of PCa, showing that the serum expression of these novel miRNAs was upregulated in PCa patients compared to controls, thus confirming their clinical relevance. Our findings highlight the potential of using ultrasound to identify novel cell-free miRNAs released from cancer cells, with the aim of developing new biomarkers with diagnostic and predictive value.Jessica CorniceDaria CapeceMauro Di Vito NolfiMonica Di PadovaChiara CompagnoniDaniela VerzellaBarbara Di FrancescoDavide VecchiottiIrene FlatiAlessandra TessitoreEdoardo AlesseGaetano BarbatoFrancesca ZazzeroniMDPI AGarticlemiRNAsbiomarkersprostate cancerultrasoundsGeneticsQH426-470ENGenes, Vol 12, Iss 1726, p 1726 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	miRNAs biomarkers prostate cancer ultrasounds Genetics QH426-470
spellingShingle	miRNAs biomarkers prostate cancer ultrasounds Genetics QH426-470 Jessica Cornice Daria Capece Mauro Di Vito Nolfi Monica Di Padova Chiara Compagnoni Daniela Verzella Barbara Di Francesco Davide Vecchiotti Irene Flati Alessandra Tessitore Edoardo Alesse Gaetano Barbato Francesca Zazzeroni Ultrasound-Based Method for the Identification of Novel MicroRNA Biomarkers in Prostate Cancer
description	The detection of circulating microRNA (miRNA)-based biomarkers represents an innovative, non-invasive method for the early detection of cancer. However, the low concentration of miRNAs released in body fluids and the difficult identification of the tumor site have limited their clinical use as effective cancer biomarkers. To evaluate if ultrasound treatment could amplify the release of extracellular cancer biomarkers, we treated a panel of prostate cancer (PCa) cell lines with an ultrasound-based prototype and profiled the release of miRNAs in the extracellular space, with the aim of identifying novel miRNA-based biomarkers that could be used for PCa diagnosis and the monitoring of tumor evolution. We provide evidence that US-mediated sonoporation amplifies the release of miRNAs from both androgen-dependent (AD) and -independent (AI) PCa cells. We identified four PCa-related miRNAs, whose levels in LNCaP and DU145 supernatants were significantly increased following ultrasound treatment: mir-629-5p, mir-374-5p, mir-194-5p, and let-7d-5p. We further analyzed a publicly available dataset of PCa, showing that the serum expression of these novel miRNAs was upregulated in PCa patients compared to controls, thus confirming their clinical relevance. Our findings highlight the potential of using ultrasound to identify novel cell-free miRNAs released from cancer cells, with the aim of developing new biomarkers with diagnostic and predictive value.
format	article
author	Jessica Cornice Daria Capece Mauro Di Vito Nolfi Monica Di Padova Chiara Compagnoni Daniela Verzella Barbara Di Francesco Davide Vecchiotti Irene Flati Alessandra Tessitore Edoardo Alesse Gaetano Barbato Francesca Zazzeroni
author_facet	Jessica Cornice Daria Capece Mauro Di Vito Nolfi Monica Di Padova Chiara Compagnoni Daniela Verzella Barbara Di Francesco Davide Vecchiotti Irene Flati Alessandra Tessitore Edoardo Alesse Gaetano Barbato Francesca Zazzeroni
author_sort	Jessica Cornice
title	Ultrasound-Based Method for the Identification of Novel MicroRNA Biomarkers in Prostate Cancer
title_short	Ultrasound-Based Method for the Identification of Novel MicroRNA Biomarkers in Prostate Cancer
title_full	Ultrasound-Based Method for the Identification of Novel MicroRNA Biomarkers in Prostate Cancer
title_fullStr	Ultrasound-Based Method for the Identification of Novel MicroRNA Biomarkers in Prostate Cancer
title_full_unstemmed	Ultrasound-Based Method for the Identification of Novel MicroRNA Biomarkers in Prostate Cancer
title_sort	ultrasound-based method for the identification of novel microrna biomarkers in prostate cancer
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4f632102159d48e6b95e131f44d658ec
work_keys_str_mv	AT jessicacornice ultrasoundbasedmethodfortheidentificationofnovelmicrornabiomarkersinprostatecancer AT dariacapece ultrasoundbasedmethodfortheidentificationofnovelmicrornabiomarkersinprostatecancer AT maurodivitonolfi ultrasoundbasedmethodfortheidentificationofnovelmicrornabiomarkersinprostatecancer AT monicadipadova ultrasoundbasedmethodfortheidentificationofnovelmicrornabiomarkersinprostatecancer AT chiaracompagnoni ultrasoundbasedmethodfortheidentificationofnovelmicrornabiomarkersinprostatecancer AT danielaverzella ultrasoundbasedmethodfortheidentificationofnovelmicrornabiomarkersinprostatecancer AT barbaradifrancesco ultrasoundbasedmethodfortheidentificationofnovelmicrornabiomarkersinprostatecancer AT davidevecchiotti ultrasoundbasedmethodfortheidentificationofnovelmicrornabiomarkersinprostatecancer AT ireneflati ultrasoundbasedmethodfortheidentificationofnovelmicrornabiomarkersinprostatecancer AT alessandratessitore ultrasoundbasedmethodfortheidentificationofnovelmicrornabiomarkersinprostatecancer AT edoardoalesse ultrasoundbasedmethodfortheidentificationofnovelmicrornabiomarkersinprostatecancer AT gaetanobarbato ultrasoundbasedmethodfortheidentificationofnovelmicrornabiomarkersinprostatecancer AT francescazazzeroni ultrasoundbasedmethodfortheidentificationofnovelmicrornabiomarkersinprostatecancer
_version_	1718412102621724672

Ultrasound-Based Method for the Identification of Novel MicroRNA Biomarkers in Prostate Cancer

Ejemplares similares