Dual regulation of water retention and cell growth by a stress-associated protein (SAP) gene in Prunus

Abstract We have identified a gene (PpSAP1) of Prunus persica coding for a stress-associated protein (SAP) containing Zn-finger domains A20 and AN1. SAPs have been described as regulators of the abiotic stress response in plant species, emerging as potential candidates for improvement of stress tole...

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Autores principales:	Alba Lloret, Ana Conejero, Carmen Leida, César Petri, Francisco Gil-Muñoz, Lorenzo Burgos, María Luisa Badenes, Gabino Ríos
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4f63c4695a7742aa93e46064b15a088c
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spelling	oai:doaj.org-article:4f63c4695a7742aa93e46064b15a088c2021-12-02T12:32:15ZDual regulation of water retention and cell growth by a stress-associated protein (SAP) gene in Prunus10.1038/s41598-017-00471-72045-2322https://doaj.org/article/4f63c4695a7742aa93e46064b15a088c2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00471-7https://doaj.org/toc/2045-2322Abstract We have identified a gene (PpSAP1) of Prunus persica coding for a stress-associated protein (SAP) containing Zn-finger domains A20 and AN1. SAPs have been described as regulators of the abiotic stress response in plant species, emerging as potential candidates for improvement of stress tolerance in plants. PpSAP1 was highly expressed in leaves and dormant buds, being down-regulated before bud dormancy release. PpSAP1 expression was moderately induced by water stresses and heat in buds. In addition, it was found that PpSAP1 strongly interacts with polyubiquitin proteins in the yeast two-hybrid system. The overexpression of PpSAP1 in transgenic plum plants led to alterations in leaf shape and an increase of water retention under drought stress. Moreover, we established that leaf morphological alterations were concomitant with a reduced cell size and down-regulation of genes involved in cell growth, such as GROWTH-REGULATING FACTOR (GRF)1-like, TONOPLAST INTRINSIC PROTEIN (TIP)-like, and TARGET OF RAPAMYCIN (TOR)-like. Especially, the inverse expression pattern of PpSAP1 and TOR-like in transgenic plum and peach buds suggests a role of PpSAP1 in cell expansion through the regulation of TOR pathway.Alba LloretAna ConejeroCarmen LeidaCésar PetriFrancisco Gil-MuñozLorenzo BurgosMaría Luisa BadenesGabino RíosNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alba Lloret Ana Conejero Carmen Leida César Petri Francisco Gil-Muñoz Lorenzo Burgos María Luisa Badenes Gabino Ríos Dual regulation of water retention and cell growth by a stress-associated protein (SAP) gene in Prunus
description	Abstract We have identified a gene (PpSAP1) of Prunus persica coding for a stress-associated protein (SAP) containing Zn-finger domains A20 and AN1. SAPs have been described as regulators of the abiotic stress response in plant species, emerging as potential candidates for improvement of stress tolerance in plants. PpSAP1 was highly expressed in leaves and dormant buds, being down-regulated before bud dormancy release. PpSAP1 expression was moderately induced by water stresses and heat in buds. In addition, it was found that PpSAP1 strongly interacts with polyubiquitin proteins in the yeast two-hybrid system. The overexpression of PpSAP1 in transgenic plum plants led to alterations in leaf shape and an increase of water retention under drought stress. Moreover, we established that leaf morphological alterations were concomitant with a reduced cell size and down-regulation of genes involved in cell growth, such as GROWTH-REGULATING FACTOR (GRF)1-like, TONOPLAST INTRINSIC PROTEIN (TIP)-like, and TARGET OF RAPAMYCIN (TOR)-like. Especially, the inverse expression pattern of PpSAP1 and TOR-like in transgenic plum and peach buds suggests a role of PpSAP1 in cell expansion through the regulation of TOR pathway.
format	article
author	Alba Lloret Ana Conejero Carmen Leida César Petri Francisco Gil-Muñoz Lorenzo Burgos María Luisa Badenes Gabino Ríos
author_facet	Alba Lloret Ana Conejero Carmen Leida César Petri Francisco Gil-Muñoz Lorenzo Burgos María Luisa Badenes Gabino Ríos
author_sort	Alba Lloret
title	Dual regulation of water retention and cell growth by a stress-associated protein (SAP) gene in Prunus
title_short	Dual regulation of water retention and cell growth by a stress-associated protein (SAP) gene in Prunus
title_full	Dual regulation of water retention and cell growth by a stress-associated protein (SAP) gene in Prunus
title_fullStr	Dual regulation of water retention and cell growth by a stress-associated protein (SAP) gene in Prunus
title_full_unstemmed	Dual regulation of water retention and cell growth by a stress-associated protein (SAP) gene in Prunus
title_sort	dual regulation of water retention and cell growth by a stress-associated protein (sap) gene in prunus
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4f63c4695a7742aa93e46064b15a088c
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Dual regulation of water retention and cell growth by a stress-associated protein (SAP) gene in Prunus

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