Long non-coding RNA FENDRR inhibits the stemenss of colorectal cancer cells through directly binding to Sox2 RNA

Cancer stem cells (CSCs) contribute to malignant features. Long non-coding RNA (LncRNA) FENDRR has been shown to regulate tumor proliferation, migration, and invasion. However, the effects of FENDRR on the CSC-like traits of colorectal cancer cells remain to be elucidated. Here, we identified that l...

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Autores principales:	Xin Zhao, Jincheng Wu, Yongwen Li, Feng Ye, Chunyue Wang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	long non-coding rna sox2 cancer stem cell mrna stability rna–rna interaction Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/4f665305ae514178bb07ad64a83ae0ac
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spelling	oai:doaj.org-article:4f665305ae514178bb07ad64a83ae0ac2021-11-04T15:51:53ZLong non-coding RNA FENDRR inhibits the stemenss of colorectal cancer cells through directly binding to Sox2 RNA2165-59792165-598710.1080/21655979.2021.1977054https://doaj.org/article/4f665305ae514178bb07ad64a83ae0ac2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1977054https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Cancer stem cells (CSCs) contribute to malignant features. Long non-coding RNA (LncRNA) FENDRR has been shown to regulate tumor proliferation, migration, and invasion. However, the effects of FENDRR on the CSC-like traits of colorectal cancer cells remain to be elucidated. Here, we identified that lncRNA FENDRR level was remarkably lower in spheres formed by colorectal cancer cells compared to that in parental cancer cells. Further functional experiments showed that FENDRR overexpression attenuated the CSC-like traits of colorectal cancer spheres, while FENDRR knockdown conferred the CSC-like traits for colorectal cancer cells, as characterized by the alteration of ALDH activity, sphere-formation ability, and expression of stemness markers (Oct4, Sox2, and KLF4). RNA–RNA interaction in vitro analysis combined with mRNA stability assay revealed that lncRNA FENDRR directly interacted with Sox2 mRNA 3’UTR, reduced its mRNA stability and thus inhibited Sox2 expression. In addition, lncRNA FENDRR-mediated effects on the CSC-like traits of colorectal cancer cells depended on Sox2 expression. This work suggests that lncRNA FENDRR can block the CSC-like traits in colorectal cancer cells through directly interacting with Sox2 mRNA 3’UTR.Xin ZhaoJincheng WuYongwen LiFeng YeChunyue WangTaylor & Francis Grouparticlelong non-coding rnasox2cancer stem cellmrna stabilityrna–rna interactionBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 8698-8708 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	long non-coding rna sox2 cancer stem cell mrna stability rna–rna interaction Biotechnology TP248.13-248.65
spellingShingle	long non-coding rna sox2 cancer stem cell mrna stability rna–rna interaction Biotechnology TP248.13-248.65 Xin Zhao Jincheng Wu Yongwen Li Feng Ye Chunyue Wang Long non-coding RNA FENDRR inhibits the stemenss of colorectal cancer cells through directly binding to Sox2 RNA
description	Cancer stem cells (CSCs) contribute to malignant features. Long non-coding RNA (LncRNA) FENDRR has been shown to regulate tumor proliferation, migration, and invasion. However, the effects of FENDRR on the CSC-like traits of colorectal cancer cells remain to be elucidated. Here, we identified that lncRNA FENDRR level was remarkably lower in spheres formed by colorectal cancer cells compared to that in parental cancer cells. Further functional experiments showed that FENDRR overexpression attenuated the CSC-like traits of colorectal cancer spheres, while FENDRR knockdown conferred the CSC-like traits for colorectal cancer cells, as characterized by the alteration of ALDH activity, sphere-formation ability, and expression of stemness markers (Oct4, Sox2, and KLF4). RNA–RNA interaction in vitro analysis combined with mRNA stability assay revealed that lncRNA FENDRR directly interacted with Sox2 mRNA 3’UTR, reduced its mRNA stability and thus inhibited Sox2 expression. In addition, lncRNA FENDRR-mediated effects on the CSC-like traits of colorectal cancer cells depended on Sox2 expression. This work suggests that lncRNA FENDRR can block the CSC-like traits in colorectal cancer cells through directly interacting with Sox2 mRNA 3’UTR.
format	article
author	Xin Zhao Jincheng Wu Yongwen Li Feng Ye Chunyue Wang
author_facet	Xin Zhao Jincheng Wu Yongwen Li Feng Ye Chunyue Wang
author_sort	Xin Zhao
title	Long non-coding RNA FENDRR inhibits the stemenss of colorectal cancer cells through directly binding to Sox2 RNA
title_short	Long non-coding RNA FENDRR inhibits the stemenss of colorectal cancer cells through directly binding to Sox2 RNA
title_full	Long non-coding RNA FENDRR inhibits the stemenss of colorectal cancer cells through directly binding to Sox2 RNA
title_fullStr	Long non-coding RNA FENDRR inhibits the stemenss of colorectal cancer cells through directly binding to Sox2 RNA
title_full_unstemmed	Long non-coding RNA FENDRR inhibits the stemenss of colorectal cancer cells through directly binding to Sox2 RNA
title_sort	long non-coding rna fendrr inhibits the stemenss of colorectal cancer cells through directly binding to sox2 rna
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/4f665305ae514178bb07ad64a83ae0ac
work_keys_str_mv	AT xinzhao longnoncodingrnafendrrinhibitsthestemenssofcolorectalcancercellsthroughdirectlybindingtosox2rna AT jinchengwu longnoncodingrnafendrrinhibitsthestemenssofcolorectalcancercellsthroughdirectlybindingtosox2rna AT yongwenli longnoncodingrnafendrrinhibitsthestemenssofcolorectalcancercellsthroughdirectlybindingtosox2rna AT fengye longnoncodingrnafendrrinhibitsthestemenssofcolorectalcancercellsthroughdirectlybindingtosox2rna AT chunyuewang longnoncodingrnafendrrinhibitsthestemenssofcolorectalcancercellsthroughdirectlybindingtosox2rna
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Long non-coding RNA FENDRR inhibits the stemenss of colorectal cancer cells through directly binding to Sox2 RNA

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