Real-time cancer diagnosis of breast cancer using fluorescence lifetime endoscopy based on the pH

Abstract A biopsy is often performed for the diagnosis of cancer during a surgical operation. In addition, pathological biopsy is required to discriminate the margin between cancer tissues and normal tissues in surgical specimens. In this study, we presented a novel method for discriminating between...

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Autores principales:	Jooran Lee, Byungyeon Kim, Byungjun Park, Youngjae Won, Sang-Yeob Kim, Seungrag Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4f714369ab7e42958b806d64aaf49cca
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spelling	oai:doaj.org-article:4f714369ab7e42958b806d64aaf49cca2021-12-02T15:10:50ZReal-time cancer diagnosis of breast cancer using fluorescence lifetime endoscopy based on the pH10.1038/s41598-021-96531-02045-2322https://doaj.org/article/4f714369ab7e42958b806d64aaf49cca2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96531-0https://doaj.org/toc/2045-2322Abstract A biopsy is often performed for the diagnosis of cancer during a surgical operation. In addition, pathological biopsy is required to discriminate the margin between cancer tissues and normal tissues in surgical specimens. In this study, we presented a novel method for discriminating between tumor and normal tissues using fluorescence lifetime endoscopy (FLE). We demonstrated the relationship between the fluorescence lifetime and pH in fluorescein using the proposed fluorescence lifetime measurement system. We also showed that cancer could be diagnosed based on this relationship by assessing differences in pH based fluorescence lifetime between cancer and normal tissues using two different types of tumor such as breast tumors (MDA-MB-361) and skin tumors (A375), where cancer tissues have ranged in pH from 4.5 to 7.0 and normal tissues have ranged in pH from 7.0 to 7.4. To support this approach, we performed hematoxylin and eosin (H&E) staining test of normal and cancer tissues within a certain area. From these results, we showed the ability to diagnose a cancer using FLE technique, which were consistent with the diagnosis of a cancer with H&E staining test. In summary, the proposed pH-based FLE technique could provide a real time, in vivo, and in-situ clinical diagnostic method for the cancer surgical and could be presented as an alternative to biopsy procedures.Jooran LeeByungyeon KimByungjun ParkYoungjae WonSang-Yeob KimSeungrag LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jooran Lee Byungyeon Kim Byungjun Park Youngjae Won Sang-Yeob Kim Seungrag Lee Real-time cancer diagnosis of breast cancer using fluorescence lifetime endoscopy based on the pH
description	Abstract A biopsy is often performed for the diagnosis of cancer during a surgical operation. In addition, pathological biopsy is required to discriminate the margin between cancer tissues and normal tissues in surgical specimens. In this study, we presented a novel method for discriminating between tumor and normal tissues using fluorescence lifetime endoscopy (FLE). We demonstrated the relationship between the fluorescence lifetime and pH in fluorescein using the proposed fluorescence lifetime measurement system. We also showed that cancer could be diagnosed based on this relationship by assessing differences in pH based fluorescence lifetime between cancer and normal tissues using two different types of tumor such as breast tumors (MDA-MB-361) and skin tumors (A375), where cancer tissues have ranged in pH from 4.5 to 7.0 and normal tissues have ranged in pH from 7.0 to 7.4. To support this approach, we performed hematoxylin and eosin (H&E) staining test of normal and cancer tissues within a certain area. From these results, we showed the ability to diagnose a cancer using FLE technique, which were consistent with the diagnosis of a cancer with H&E staining test. In summary, the proposed pH-based FLE technique could provide a real time, in vivo, and in-situ clinical diagnostic method for the cancer surgical and could be presented as an alternative to biopsy procedures.
format	article
author	Jooran Lee Byungyeon Kim Byungjun Park Youngjae Won Sang-Yeob Kim Seungrag Lee
author_facet	Jooran Lee Byungyeon Kim Byungjun Park Youngjae Won Sang-Yeob Kim Seungrag Lee
author_sort	Jooran Lee
title	Real-time cancer diagnosis of breast cancer using fluorescence lifetime endoscopy based on the pH
title_short	Real-time cancer diagnosis of breast cancer using fluorescence lifetime endoscopy based on the pH
title_full	Real-time cancer diagnosis of breast cancer using fluorescence lifetime endoscopy based on the pH
title_fullStr	Real-time cancer diagnosis of breast cancer using fluorescence lifetime endoscopy based on the pH
title_full_unstemmed	Real-time cancer diagnosis of breast cancer using fluorescence lifetime endoscopy based on the pH
title_sort	real-time cancer diagnosis of breast cancer using fluorescence lifetime endoscopy based on the ph
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4f714369ab7e42958b806d64aaf49cca
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_version_	1718387688514519040

Real-time cancer diagnosis of breast cancer using fluorescence lifetime endoscopy based on the pH

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