Effects of epigallocatechin-3-gallate and acyclovir on herpes simplex virus type 1 infection in oral epithelial cells

Background/purpose: Herpes simplex virus type 1 (HSV-1) is the pathogenic agent of human diseases, including gingivostomatitis and herpes labialis. The anti-viral activities of the tea polyphenol, epigallocatechin-3-gallate (EGCG), have been demonstrated. This study examined the combined effects of...

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Autores principales:	Ching-Yi Wu, Zhu-Yun Yu, Yi-Chen Chen, Shan-Ling Hung
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Acyclovir Epigallocatechin-3-gallate Herpes simplex virus type 1 Oral epithelial cells Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4f72c6a3f3ab41899e6a023efa0fe4b4
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spelling	oai:doaj.org-article:4f72c6a3f3ab41899e6a023efa0fe4b42021-12-02T04:59:08ZEffects of epigallocatechin-3-gallate and acyclovir on herpes simplex virus type 1 infection in oral epithelial cells0929-664610.1016/j.jfma.2020.12.018https://doaj.org/article/4f72c6a3f3ab41899e6a023efa0fe4b42021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0929664620306185https://doaj.org/toc/0929-6646Background/purpose: Herpes simplex virus type 1 (HSV-1) is the pathogenic agent of human diseases, including gingivostomatitis and herpes labialis. The anti-viral activities of the tea polyphenol, epigallocatechin-3-gallate (EGCG), have been demonstrated. This study examined the combined effects of EGCG and the antiviral drug, acyclovir (ACV), on infection of HSV-1 in oral epithelial cells. Methods: Cell viability was examined using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide. Viral yields were determined using the plaque assay. Viral proteins were detected using Western blotting analysis or confocal laser scanning microscopy. Viral DNA was detected using the real-time polymerase chain reaction. Results: Cytotoxic effects of HSV-1 on the viability of oral epithelial cells were evidently reduced in the presence of EGCG (25 μg/ml) or/and ACV (50 μg/ml). Viral yields were also significantly reduced by treatment of cells with EGCG or/and ACV. Expression of viral immediate early protein, infected cell protein 0 (ICP0), was greatly inhibited when cells were treated with EGCG. Combined effects of EGCG and ACV were more evident for the expression of viral thymidine kinase, ICP5 and glycoprotein D. EGCG, but not ACV, significantly reduced the levels of viral particles and viral DNA during viral entry phase. However, at 20 h post infection, the intracellular viral DNA was evidently reduced in HSV-1 infected cells treated with EGCG and ACV. Moreover, the stimulatory effects of HSV-1 on phosphorylation of c-Jun N-terminal kinase could be reduced by ACV. Conclusion: The results demonstrated the additive effects of EGCG and ACV on HSV-1 infection in oral epithelial cells.Ching-Yi WuZhu-Yun YuYi-Chen ChenShan-Ling HungElsevierarticleAcyclovirEpigallocatechin-3-gallateHerpes simplex virus type 1Oral epithelial cellsMedicine (General)R5-920ENJournal of the Formosan Medical Association, Vol 120, Iss 12, Pp 2136-2143 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Acyclovir Epigallocatechin-3-gallate Herpes simplex virus type 1 Oral epithelial cells Medicine (General) R5-920
spellingShingle	Acyclovir Epigallocatechin-3-gallate Herpes simplex virus type 1 Oral epithelial cells Medicine (General) R5-920 Ching-Yi Wu Zhu-Yun Yu Yi-Chen Chen Shan-Ling Hung Effects of epigallocatechin-3-gallate and acyclovir on herpes simplex virus type 1 infection in oral epithelial cells
description	Background/purpose: Herpes simplex virus type 1 (HSV-1) is the pathogenic agent of human diseases, including gingivostomatitis and herpes labialis. The anti-viral activities of the tea polyphenol, epigallocatechin-3-gallate (EGCG), have been demonstrated. This study examined the combined effects of EGCG and the antiviral drug, acyclovir (ACV), on infection of HSV-1 in oral epithelial cells. Methods: Cell viability was examined using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide. Viral yields were determined using the plaque assay. Viral proteins were detected using Western blotting analysis or confocal laser scanning microscopy. Viral DNA was detected using the real-time polymerase chain reaction. Results: Cytotoxic effects of HSV-1 on the viability of oral epithelial cells were evidently reduced in the presence of EGCG (25 μg/ml) or/and ACV (50 μg/ml). Viral yields were also significantly reduced by treatment of cells with EGCG or/and ACV. Expression of viral immediate early protein, infected cell protein 0 (ICP0), was greatly inhibited when cells were treated with EGCG. Combined effects of EGCG and ACV were more evident for the expression of viral thymidine kinase, ICP5 and glycoprotein D. EGCG, but not ACV, significantly reduced the levels of viral particles and viral DNA during viral entry phase. However, at 20 h post infection, the intracellular viral DNA was evidently reduced in HSV-1 infected cells treated with EGCG and ACV. Moreover, the stimulatory effects of HSV-1 on phosphorylation of c-Jun N-terminal kinase could be reduced by ACV. Conclusion: The results demonstrated the additive effects of EGCG and ACV on HSV-1 infection in oral epithelial cells.
format	article
author	Ching-Yi Wu Zhu-Yun Yu Yi-Chen Chen Shan-Ling Hung
author_facet	Ching-Yi Wu Zhu-Yun Yu Yi-Chen Chen Shan-Ling Hung
author_sort	Ching-Yi Wu
title	Effects of epigallocatechin-3-gallate and acyclovir on herpes simplex virus type 1 infection in oral epithelial cells
title_short	Effects of epigallocatechin-3-gallate and acyclovir on herpes simplex virus type 1 infection in oral epithelial cells
title_full	Effects of epigallocatechin-3-gallate and acyclovir on herpes simplex virus type 1 infection in oral epithelial cells
title_fullStr	Effects of epigallocatechin-3-gallate and acyclovir on herpes simplex virus type 1 infection in oral epithelial cells
title_full_unstemmed	Effects of epigallocatechin-3-gallate and acyclovir on herpes simplex virus type 1 infection in oral epithelial cells
title_sort	effects of epigallocatechin-3-gallate and acyclovir on herpes simplex virus type 1 infection in oral epithelial cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/4f72c6a3f3ab41899e6a023efa0fe4b4
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Effects of epigallocatechin-3-gallate and acyclovir on herpes simplex virus type 1 infection in oral epithelial cells

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