Human Papillomavirus Status of Head and Neck Squamous Cell Carcinoma Patients and its Association with Expression of PIK3CA and CRNDE

Background: Human papillomavirus (HPV) positive head and neck squamous cell carcinoma (HNSCC) have distinctive molecular features. We conducted the present work to evaluate the HPV status of HNSCC patients and its association with expression of PIK3CA and CRNDE genes. Method: In the present case-con...

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Autores principales:	Laleh Karimi Sani, Abbas Shakoori Garakani, Sayeh Jafari, Ebrahim Razmpa
Formato:	article
Lenguaje:	EN
Publicado:	Shiraz University of Medical Sciences 2021
Materias:	hpv typing gene expression reverse line blot roc curve Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/4f80a69a1e5f45228d7a18cd3ccca479
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spelling	oai:doaj.org-article:4f80a69a1e5f45228d7a18cd3ccca4792021-11-29T10:40:47ZHuman Papillomavirus Status of Head and Neck Squamous Cell Carcinoma Patients and its Association with Expression of PIK3CA and CRNDE2008-67092008-668710.30476/mejc.2021.84958.1247https://doaj.org/article/4f80a69a1e5f45228d7a18cd3ccca4792021-10-01T00:00:00Zhttps://mejc.sums.ac.ir/article_47533_f7352f92ab56b6614d4fdde45326a6f2.pdfhttps://doaj.org/toc/2008-6709https://doaj.org/toc/2008-6687Background: Human papillomavirus (HPV) positive head and neck squamous cell carcinoma (HNSCC) have distinctive molecular features. We conducted the present work to evaluate the HPV status of HNSCC patients and its association with expression of PIK3CA and CRNDE genes. Method: In the present case-control study, 50 fresh frozen tissue samples of HNSCC patients were collected from the patients referred to hospital for tumor removal. HPV typing was performed on DNA samples using a one-step polymerase chain reaction (PCR) followed by Reverse Line Blot method. Relative expression of PIK3CA and CRNDE genes was evaluated utilizing a real-time PCR method. Results: Out of 50 patients, 14 (28%) were HPV positive and the most prevalent type was HPV 16. Both PIK3CA and CRNDE genes were upregulated in tumoral tissues compared with adjacent non-cancerous tissues (ANCTs) (P=0.0322 and 0.0005, respectively). Based on the area under curve (AUC) values, the diagnostic power of CRNDE (AUC= 0.676) was higher than that of PIK3CA (AUC= 0.604). Finally, the expression level of PIK3CA was significantly associated with HPV+ HNSCC (P=0.01). Conclusion: We showed that the prevalence of HPV in HNSCC was within our local prevalence range. Moreover, the association of PIK3CA overexpression with HPV status implied distinctive molecular characteristics of HPV(+) HNSCC.Laleh Karimi SaniAbbas Shakoori GarakaniSayeh JafariEbrahim RazmpaShiraz University of Medical Sciencesarticlehpv typinggene expressionreverse line blotroc curveNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENMiddle East Journal of Cancer , Vol 12, Iss 4, Pp 473-482 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hpv typing gene expression reverse line blot roc curve Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	hpv typing gene expression reverse line blot roc curve Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Laleh Karimi Sani Abbas Shakoori Garakani Sayeh Jafari Ebrahim Razmpa Human Papillomavirus Status of Head and Neck Squamous Cell Carcinoma Patients and its Association with Expression of PIK3CA and CRNDE
description	Background: Human papillomavirus (HPV) positive head and neck squamous cell carcinoma (HNSCC) have distinctive molecular features. We conducted the present work to evaluate the HPV status of HNSCC patients and its association with expression of PIK3CA and CRNDE genes. Method: In the present case-control study, 50 fresh frozen tissue samples of HNSCC patients were collected from the patients referred to hospital for tumor removal. HPV typing was performed on DNA samples using a one-step polymerase chain reaction (PCR) followed by Reverse Line Blot method. Relative expression of PIK3CA and CRNDE genes was evaluated utilizing a real-time PCR method. Results: Out of 50 patients, 14 (28%) were HPV positive and the most prevalent type was HPV 16. Both PIK3CA and CRNDE genes were upregulated in tumoral tissues compared with adjacent non-cancerous tissues (ANCTs) (P=0.0322 and 0.0005, respectively). Based on the area under curve (AUC) values, the diagnostic power of CRNDE (AUC= 0.676) was higher than that of PIK3CA (AUC= 0.604). Finally, the expression level of PIK3CA was significantly associated with HPV+ HNSCC (P=0.01). Conclusion: We showed that the prevalence of HPV in HNSCC was within our local prevalence range. Moreover, the association of PIK3CA overexpression with HPV status implied distinctive molecular characteristics of HPV(+) HNSCC.
format	article
author	Laleh Karimi Sani Abbas Shakoori Garakani Sayeh Jafari Ebrahim Razmpa
author_facet	Laleh Karimi Sani Abbas Shakoori Garakani Sayeh Jafari Ebrahim Razmpa
author_sort	Laleh Karimi Sani
title	Human Papillomavirus Status of Head and Neck Squamous Cell Carcinoma Patients and its Association with Expression of PIK3CA and CRNDE
title_short	Human Papillomavirus Status of Head and Neck Squamous Cell Carcinoma Patients and its Association with Expression of PIK3CA and CRNDE
title_full	Human Papillomavirus Status of Head and Neck Squamous Cell Carcinoma Patients and its Association with Expression of PIK3CA and CRNDE
title_fullStr	Human Papillomavirus Status of Head and Neck Squamous Cell Carcinoma Patients and its Association with Expression of PIK3CA and CRNDE
title_full_unstemmed	Human Papillomavirus Status of Head and Neck Squamous Cell Carcinoma Patients and its Association with Expression of PIK3CA and CRNDE
title_sort	human papillomavirus status of head and neck squamous cell carcinoma patients and its association with expression of pik3ca and crnde
publisher	Shiraz University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/4f80a69a1e5f45228d7a18cd3ccca479
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Human Papillomavirus Status of Head and Neck Squamous Cell Carcinoma Patients and its Association with Expression of PIK3CA and CRNDE

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