Angiotensin II upregulates endothelial lipase expression via the NF-kappa B and MAPK signaling pathways.

<h4>Background</h4>Angiotensin II (AngII) participates in endothelial damage and inflammation, and accelerates atherosclerosis. Endothelial lipase (EL) is involved in the metabolism and clearance of high density lipoproteins (HDL), the serum levels of which correlate negatively with the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiaoli Zhang, Minghui Wu, Hong Jiang, Jing Hao, Qingli Zhang, Qing Zhu, Gaowa Saren, Yun Zhang, Xiaohui Meng, Xin Yue
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4f8fc572e51442bb99b15b790714a616
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4f8fc572e51442bb99b15b790714a616
record_format	dspace
spelling	oai:doaj.org-article:4f8fc572e51442bb99b15b790714a6162021-11-25T05:59:32ZAngiotensin II upregulates endothelial lipase expression via the NF-kappa B and MAPK signaling pathways.1932-620310.1371/journal.pone.0107634https://doaj.org/article/4f8fc572e51442bb99b15b790714a6162014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0107634https://doaj.org/toc/1932-6203<h4>Background</h4>Angiotensin II (AngII) participates in endothelial damage and inflammation, and accelerates atherosclerosis. Endothelial lipase (EL) is involved in the metabolism and clearance of high density lipoproteins (HDL), the serum levels of which correlate negatively with the onset of cardiovascular diseases including atherosclerosis. However, the relationship between AngII and EL is not yet fully understood. In this study, we investigated the effects of AngII on the expression of EL and the signaling pathways that mediate its effects in human umbilical vein endothelial cells (HUVECs).<h4>Methods and findings</h4>HUVECs were cultured in vitro with different treatments as follows: 1) The control group without any treatment; 2) AngII treatment for 0 h, 4 h, 8 h, 12 h and 24 h; 3) NF-κB activation inhibitor pyrrolidine dithiocarbamate (PDTC) pretreatment for 1 h before AngII treatment; and 4) mitogen-activated protein kinase (MAPK) p38 inhibitor (SB203580) pretreatment for 1 h before AngII treatment. EL levels in each group were detected by immunocytochemical staining and western blotting. HUVECs proliferation was detected by MTT and proliferating cell nuclear antigen (PCNA) immunofluorescence staining. NF-kappa B (NF-κB) p65, MAPK p38, c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and phosphorylated extracellular signal-regulated kinase (p-ERK) expression levels were assayed by western blotting. The results showed that the protein levels of EL, NF-κB p65, MAPK p38, JNK, and p-ERK protein levels, in addition to the proliferation of HUVECs, were increased by AngII. Both the NF-kB inhibitor (PDTC) and the MAPK p38 inhibitor (SB203580) partially inhibited the effects of AngII on EL expression.<h4>Conclusion</h4>AngII may upregulate EL protein expression via the NF-κB and MAPK signaling pathways.Xiaoli ZhangMinghui WuHong JiangJing HaoQingli ZhangQing ZhuGaowa SarenYun ZhangXiaohui MengXin YuePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e107634 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaoli Zhang Minghui Wu Hong Jiang Jing Hao Qingli Zhang Qing Zhu Gaowa Saren Yun Zhang Xiaohui Meng Xin Yue Angiotensin II upregulates endothelial lipase expression via the NF-kappa B and MAPK signaling pathways.
description	<h4>Background</h4>Angiotensin II (AngII) participates in endothelial damage and inflammation, and accelerates atherosclerosis. Endothelial lipase (EL) is involved in the metabolism and clearance of high density lipoproteins (HDL), the serum levels of which correlate negatively with the onset of cardiovascular diseases including atherosclerosis. However, the relationship between AngII and EL is not yet fully understood. In this study, we investigated the effects of AngII on the expression of EL and the signaling pathways that mediate its effects in human umbilical vein endothelial cells (HUVECs).<h4>Methods and findings</h4>HUVECs were cultured in vitro with different treatments as follows: 1) The control group without any treatment; 2) AngII treatment for 0 h, 4 h, 8 h, 12 h and 24 h; 3) NF-κB activation inhibitor pyrrolidine dithiocarbamate (PDTC) pretreatment for 1 h before AngII treatment; and 4) mitogen-activated protein kinase (MAPK) p38 inhibitor (SB203580) pretreatment for 1 h before AngII treatment. EL levels in each group were detected by immunocytochemical staining and western blotting. HUVECs proliferation was detected by MTT and proliferating cell nuclear antigen (PCNA) immunofluorescence staining. NF-kappa B (NF-κB) p65, MAPK p38, c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and phosphorylated extracellular signal-regulated kinase (p-ERK) expression levels were assayed by western blotting. The results showed that the protein levels of EL, NF-κB p65, MAPK p38, JNK, and p-ERK protein levels, in addition to the proliferation of HUVECs, were increased by AngII. Both the NF-kB inhibitor (PDTC) and the MAPK p38 inhibitor (SB203580) partially inhibited the effects of AngII on EL expression.<h4>Conclusion</h4>AngII may upregulate EL protein expression via the NF-κB and MAPK signaling pathways.
format	article
author	Xiaoli Zhang Minghui Wu Hong Jiang Jing Hao Qingli Zhang Qing Zhu Gaowa Saren Yun Zhang Xiaohui Meng Xin Yue
author_facet	Xiaoli Zhang Minghui Wu Hong Jiang Jing Hao Qingli Zhang Qing Zhu Gaowa Saren Yun Zhang Xiaohui Meng Xin Yue
author_sort	Xiaoli Zhang
title	Angiotensin II upregulates endothelial lipase expression via the NF-kappa B and MAPK signaling pathways.
title_short	Angiotensin II upregulates endothelial lipase expression via the NF-kappa B and MAPK signaling pathways.
title_full	Angiotensin II upregulates endothelial lipase expression via the NF-kappa B and MAPK signaling pathways.
title_fullStr	Angiotensin II upregulates endothelial lipase expression via the NF-kappa B and MAPK signaling pathways.
title_full_unstemmed	Angiotensin II upregulates endothelial lipase expression via the NF-kappa B and MAPK signaling pathways.
title_sort	angiotensin ii upregulates endothelial lipase expression via the nf-kappa b and mapk signaling pathways.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/4f8fc572e51442bb99b15b790714a616
work_keys_str_mv	AT xiaolizhang angiotensiniiupregulatesendotheliallipaseexpressionviathenfkappabandmapksignalingpathways AT minghuiwu angiotensiniiupregulatesendotheliallipaseexpressionviathenfkappabandmapksignalingpathways AT hongjiang angiotensiniiupregulatesendotheliallipaseexpressionviathenfkappabandmapksignalingpathways AT jinghao angiotensiniiupregulatesendotheliallipaseexpressionviathenfkappabandmapksignalingpathways AT qinglizhang angiotensiniiupregulatesendotheliallipaseexpressionviathenfkappabandmapksignalingpathways AT qingzhu angiotensiniiupregulatesendotheliallipaseexpressionviathenfkappabandmapksignalingpathways AT gaowasaren angiotensiniiupregulatesendotheliallipaseexpressionviathenfkappabandmapksignalingpathways AT yunzhang angiotensiniiupregulatesendotheliallipaseexpressionviathenfkappabandmapksignalingpathways AT xiaohuimeng angiotensiniiupregulatesendotheliallipaseexpressionviathenfkappabandmapksignalingpathways AT xinyue angiotensiniiupregulatesendotheliallipaseexpressionviathenfkappabandmapksignalingpathways
_version_	1718414280407121920

Angiotensin II upregulates endothelial lipase expression via the NF-kappa B and MAPK signaling pathways.

Ejemplares similares