Occurrence and molecular characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia.

Potato cyst nematodes (PCN) from the genus Globodera spp. cause major losses in the potato (Solanum tuberosum) industry worldwide. Despite their importance, at present little is known about the status of this plant pathogen in cultivated potatoes in Colombia. In this study, a total of 589 samples co...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Daniela Vallejo, Diego A Rojas, John A Martinez, Sergio Marchant, Claudia M Holguin, Olga Y Pérez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4f90c984062e441487729f6f8a010155
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4f90c984062e441487729f6f8a010155
record_format	dspace
spelling	oai:doaj.org-article:4f90c984062e441487729f6f8a0101552021-12-02T20:05:04ZOccurrence and molecular characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia.1932-620310.1371/journal.pone.0241256https://doaj.org/article/4f90c984062e441487729f6f8a0101552021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0241256https://doaj.org/toc/1932-6203Potato cyst nematodes (PCN) from the genus Globodera spp. cause major losses in the potato (Solanum tuberosum) industry worldwide. Despite their importance, at present little is known about the status of this plant pathogen in cultivated potatoes in Colombia. In this study, a total of 589 samples collected from 75 geographic localities in nine potato producing regions of Colombia (Cundinamarca, Boyacá, Antioquia, Nariño, Santander, Norte de Santander, Tolima, Caldas and Cauca) were assayed for the presence of potato cyst nematodes. Fifty-seven percent of samples tested positive for PCN. Based on phylogenetic analysis of the internal transcribed spacer region (ITS1-5.8S-ITS2) of the rRNA gene and D2-D3 expansion segments of the 28S rRNA gene, all populations but one were identified as Globodera pallida. Sequences of G. pallida from Colombia formed a monophyletic group closely related to Peruvian populations, with the lowest average number of nucleotide substitutions per site (Dxy = 0.002) and net nucleotide substitutions per site (Da = 0.001), when compared to G. pallida populations from Europe, South and North America. A single sample formed a well-supported subclade along with G. rostochiensis and G. tabacum from Japan, USA and Argentina. To our knowledge this is the first comprehensive survey of Globodera populations from Colombia that includes genetic data. Our findings on species diversity and phylogenetic relationships of Globodera populations from Colombia may help elucidate the status and distribution of Globodera species, and lead to the development of accurate management strategies for the potato cyst nematodes.Daniela VallejoDiego A RojasJohn A MartinezSergio MarchantClaudia M HolguinOlga Y PérezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0241256 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniela Vallejo Diego A Rojas John A Martinez Sergio Marchant Claudia M Holguin Olga Y Pérez Occurrence and molecular characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia.
description	Potato cyst nematodes (PCN) from the genus Globodera spp. cause major losses in the potato (Solanum tuberosum) industry worldwide. Despite their importance, at present little is known about the status of this plant pathogen in cultivated potatoes in Colombia. In this study, a total of 589 samples collected from 75 geographic localities in nine potato producing regions of Colombia (Cundinamarca, Boyacá, Antioquia, Nariño, Santander, Norte de Santander, Tolima, Caldas and Cauca) were assayed for the presence of potato cyst nematodes. Fifty-seven percent of samples tested positive for PCN. Based on phylogenetic analysis of the internal transcribed spacer region (ITS1-5.8S-ITS2) of the rRNA gene and D2-D3 expansion segments of the 28S rRNA gene, all populations but one were identified as Globodera pallida. Sequences of G. pallida from Colombia formed a monophyletic group closely related to Peruvian populations, with the lowest average number of nucleotide substitutions per site (Dxy = 0.002) and net nucleotide substitutions per site (Da = 0.001), when compared to G. pallida populations from Europe, South and North America. A single sample formed a well-supported subclade along with G. rostochiensis and G. tabacum from Japan, USA and Argentina. To our knowledge this is the first comprehensive survey of Globodera populations from Colombia that includes genetic data. Our findings on species diversity and phylogenetic relationships of Globodera populations from Colombia may help elucidate the status and distribution of Globodera species, and lead to the development of accurate management strategies for the potato cyst nematodes.
format	article
author	Daniela Vallejo Diego A Rojas John A Martinez Sergio Marchant Claudia M Holguin Olga Y Pérez
author_facet	Daniela Vallejo Diego A Rojas John A Martinez Sergio Marchant Claudia M Holguin Olga Y Pérez
author_sort	Daniela Vallejo
title	Occurrence and molecular characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia.
title_short	Occurrence and molecular characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia.
title_full	Occurrence and molecular characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia.
title_fullStr	Occurrence and molecular characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia.
title_full_unstemmed	Occurrence and molecular characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia.
title_sort	occurrence and molecular characterization of cyst nematode species (globodera spp.) associated with potato crops in colombia.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/4f90c984062e441487729f6f8a010155
work_keys_str_mv	AT danielavallejo occurrenceandmolecularcharacterizationofcystnematodespeciesgloboderasppassociatedwithpotatocropsincolombia AT diegoarojas occurrenceandmolecularcharacterizationofcystnematodespeciesgloboderasppassociatedwithpotatocropsincolombia AT johnamartinez occurrenceandmolecularcharacterizationofcystnematodespeciesgloboderasppassociatedwithpotatocropsincolombia AT sergiomarchant occurrenceandmolecularcharacterizationofcystnematodespeciesgloboderasppassociatedwithpotatocropsincolombia AT claudiamholguin occurrenceandmolecularcharacterizationofcystnematodespeciesgloboderasppassociatedwithpotatocropsincolombia AT olgayperez occurrenceandmolecularcharacterizationofcystnematodespeciesgloboderasppassociatedwithpotatocropsincolombia
_version_	1718375478738288640

Occurrence and molecular characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia.

Ejemplares similares