Visualization and Quantification of the Extracellular Matrix in Prostate Cancer Using an Elastin Specific Molecular Probe

Human prostate cancer (PCa) is a type of malignancy and one of the most frequently diagnosed cancers in men. Elastin is an important component of the extracellular matrix and is involved in the structure and organization of prostate tissue. The present study examined prostate cancer in a xenograft m...

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Autores principales:	Avan Kader, Julia Brangsch, Carolin Reimann, Jan O. Kaufmann, Dilyana B. Mangarova, Jana Moeckel, Lisa C. Adams, Jing Zhao, Jessica Saatz, Heike Traub, Rebecca Buchholz, Uwe Karst, Bernd Hamm, Marcus R. Makowski
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	magnetic resonance imaging molecular imaging prostate cancer Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4f93d5471fdf409b963d4d318a415b80
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spelling	oai:doaj.org-article:4f93d5471fdf409b963d4d318a415b802021-11-25T16:48:15ZVisualization and Quantification of the Extracellular Matrix in Prostate Cancer Using an Elastin Specific Molecular Probe10.3390/biology101112172079-7737https://doaj.org/article/4f93d5471fdf409b963d4d318a415b802021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1217https://doaj.org/toc/2079-7737Human prostate cancer (PCa) is a type of malignancy and one of the most frequently diagnosed cancers in men. Elastin is an important component of the extracellular matrix and is involved in the structure and organization of prostate tissue. The present study examined prostate cancer in a xenograft mouse model using an elastin-specific molecular probe for magnetic resonance molecular imaging. Two different tumor sizes (500 mm<sup>3</sup> and 1000 mm<sup>3</sup>) were compared and analyzed by MRI in vivo and histologically and analytically ex vivo. The T1-weighted sequence was used in a clinical 3-T scanner to calculate the relative contrast enhancement before and after probe administration. Our results show that the use of an elastin-specific probe enables better discrimination between tumors and surrounding healthy tissue. Furthermore, specific binding of the probe to elastin fibers was confirmed by histological examination and laser ablation–inductively coupled plasma–mass spectrometry (LA-ICP-MS). Smaller tumors showed significantly higher signal intensity (<i>p</i> > 0.001), which correlates with the higher proportion of elastin fibers in the histological evaluation than in larger tumors. A strong correlation was seen between relative enhancement (RE) and Elastica–van Gieson staining (R2 = 0.88). RE was related to inductively coupled plasma–mass spectrometry data for Gd and showed a correlation (R2 = 0.78). Thus, molecular MRI could become a novel quantitative tool for the early evaluation and detection of PCa.Avan KaderJulia BrangschCarolin ReimannJan O. KaufmannDilyana B. MangarovaJana MoeckelLisa C. AdamsJing ZhaoJessica SaatzHeike TraubRebecca BuchholzUwe KarstBernd HammMarcus R. MakowskiMDPI AGarticlemagnetic resonance imagingmolecular imagingprostate cancerBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1217, p 1217 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	magnetic resonance imaging molecular imaging prostate cancer Biology (General) QH301-705.5
spellingShingle	magnetic resonance imaging molecular imaging prostate cancer Biology (General) QH301-705.5 Avan Kader Julia Brangsch Carolin Reimann Jan O. Kaufmann Dilyana B. Mangarova Jana Moeckel Lisa C. Adams Jing Zhao Jessica Saatz Heike Traub Rebecca Buchholz Uwe Karst Bernd Hamm Marcus R. Makowski Visualization and Quantification of the Extracellular Matrix in Prostate Cancer Using an Elastin Specific Molecular Probe
description	Human prostate cancer (PCa) is a type of malignancy and one of the most frequently diagnosed cancers in men. Elastin is an important component of the extracellular matrix and is involved in the structure and organization of prostate tissue. The present study examined prostate cancer in a xenograft mouse model using an elastin-specific molecular probe for magnetic resonance molecular imaging. Two different tumor sizes (500 mm<sup>3</sup> and 1000 mm<sup>3</sup>) were compared and analyzed by MRI in vivo and histologically and analytically ex vivo. The T1-weighted sequence was used in a clinical 3-T scanner to calculate the relative contrast enhancement before and after probe administration. Our results show that the use of an elastin-specific probe enables better discrimination between tumors and surrounding healthy tissue. Furthermore, specific binding of the probe to elastin fibers was confirmed by histological examination and laser ablation–inductively coupled plasma–mass spectrometry (LA-ICP-MS). Smaller tumors showed significantly higher signal intensity (<i>p</i> > 0.001), which correlates with the higher proportion of elastin fibers in the histological evaluation than in larger tumors. A strong correlation was seen between relative enhancement (RE) and Elastica–van Gieson staining (R2 = 0.88). RE was related to inductively coupled plasma–mass spectrometry data for Gd and showed a correlation (R2 = 0.78). Thus, molecular MRI could become a novel quantitative tool for the early evaluation and detection of PCa.
format	article
author	Avan Kader Julia Brangsch Carolin Reimann Jan O. Kaufmann Dilyana B. Mangarova Jana Moeckel Lisa C. Adams Jing Zhao Jessica Saatz Heike Traub Rebecca Buchholz Uwe Karst Bernd Hamm Marcus R. Makowski
author_facet	Avan Kader Julia Brangsch Carolin Reimann Jan O. Kaufmann Dilyana B. Mangarova Jana Moeckel Lisa C. Adams Jing Zhao Jessica Saatz Heike Traub Rebecca Buchholz Uwe Karst Bernd Hamm Marcus R. Makowski
author_sort	Avan Kader
title	Visualization and Quantification of the Extracellular Matrix in Prostate Cancer Using an Elastin Specific Molecular Probe
title_short	Visualization and Quantification of the Extracellular Matrix in Prostate Cancer Using an Elastin Specific Molecular Probe
title_full	Visualization and Quantification of the Extracellular Matrix in Prostate Cancer Using an Elastin Specific Molecular Probe
title_fullStr	Visualization and Quantification of the Extracellular Matrix in Prostate Cancer Using an Elastin Specific Molecular Probe
title_full_unstemmed	Visualization and Quantification of the Extracellular Matrix in Prostate Cancer Using an Elastin Specific Molecular Probe
title_sort	visualization and quantification of the extracellular matrix in prostate cancer using an elastin specific molecular probe
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4f93d5471fdf409b963d4d318a415b80
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Visualization and Quantification of the Extracellular Matrix in Prostate Cancer Using an Elastin Specific Molecular Probe

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