Urinary lipocalin protein in a female rodent with correlation to phases in the estrous cycle: an experimental study accompanied by in silico analysis.

Male urinary lipocalin family proteins, practically odorant-binding proteins but also could be pheromones by themselves, in rodents act as a shuttle for chemosignal communication and facilitate delivery of the signals for access to congeners. However, presence of this protein in urine of female rode...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Subramanian Muthukumar, Durairaj Rajesh, Ganesan Saibaba, Alagersamy Alagesan, Rengasamy Lakhsminarayanan Rengarajan, Govindaraju Archunan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4f9a60c313c345c799d4b02c30f43a97
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4f9a60c313c345c799d4b02c30f43a97
record_format	dspace
spelling	oai:doaj.org-article:4f9a60c313c345c799d4b02c30f43a972021-11-18T08:59:40ZUrinary lipocalin protein in a female rodent with correlation to phases in the estrous cycle: an experimental study accompanied by in silico analysis.1932-620310.1371/journal.pone.0071357https://doaj.org/article/4f9a60c313c345c799d4b02c30f43a972013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23967199/?tool=EBIhttps://doaj.org/toc/1932-6203Male urinary lipocalin family proteins, practically odorant-binding proteins but also could be pheromones by themselves, in rodents act as a shuttle for chemosignal communication and facilitate delivery of the signals for access to congeners. However, presence of this protein in urine of female rodents has not yet been reported. Therefore, the present investigation was carried out to find if lipocalin family protein is present in the urine of female house rat and, if so, to find whether its expression differs between the phases in the estrous cycle. The rat urinary protein was separated in single dimensional gel electrophoresis. A 14.5 kDa lipocalin protein appeared in the urine prominently during the estrus and metestrus phases compared to proestrus and diestrus phases. The expression of this protein in the urine was very low in ovariectomized rats. MALDI-TOF/MS analysis affirmed the 14.5 kDa protein as a lipocalin family protein. Analysis adopting bio-informatics tools further proved the protein as a lipocalin family member. Thus, this study for the first time demonstrated the presence of a lipocalin family protein in the urine of a female rodent and it was highly expressed during estrus phase. This lipocalin protein in female rat urine may facilitate a chemosignal function independently of a pheromone or in association with a specific pheromone.Subramanian MuthukumarDurairaj RajeshGanesan SaibabaAlagersamy AlagesanRengasamy Lakhsminarayanan RengarajanGovindaraju ArchunanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e71357 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Subramanian Muthukumar Durairaj Rajesh Ganesan Saibaba Alagersamy Alagesan Rengasamy Lakhsminarayanan Rengarajan Govindaraju Archunan Urinary lipocalin protein in a female rodent with correlation to phases in the estrous cycle: an experimental study accompanied by in silico analysis.
description	Male urinary lipocalin family proteins, practically odorant-binding proteins but also could be pheromones by themselves, in rodents act as a shuttle for chemosignal communication and facilitate delivery of the signals for access to congeners. However, presence of this protein in urine of female rodents has not yet been reported. Therefore, the present investigation was carried out to find if lipocalin family protein is present in the urine of female house rat and, if so, to find whether its expression differs between the phases in the estrous cycle. The rat urinary protein was separated in single dimensional gel electrophoresis. A 14.5 kDa lipocalin protein appeared in the urine prominently during the estrus and metestrus phases compared to proestrus and diestrus phases. The expression of this protein in the urine was very low in ovariectomized rats. MALDI-TOF/MS analysis affirmed the 14.5 kDa protein as a lipocalin family protein. Analysis adopting bio-informatics tools further proved the protein as a lipocalin family member. Thus, this study for the first time demonstrated the presence of a lipocalin family protein in the urine of a female rodent and it was highly expressed during estrus phase. This lipocalin protein in female rat urine may facilitate a chemosignal function independently of a pheromone or in association with a specific pheromone.
format	article
author	Subramanian Muthukumar Durairaj Rajesh Ganesan Saibaba Alagersamy Alagesan Rengasamy Lakhsminarayanan Rengarajan Govindaraju Archunan
author_facet	Subramanian Muthukumar Durairaj Rajesh Ganesan Saibaba Alagersamy Alagesan Rengasamy Lakhsminarayanan Rengarajan Govindaraju Archunan
author_sort	Subramanian Muthukumar
title	Urinary lipocalin protein in a female rodent with correlation to phases in the estrous cycle: an experimental study accompanied by in silico analysis.
title_short	Urinary lipocalin protein in a female rodent with correlation to phases in the estrous cycle: an experimental study accompanied by in silico analysis.
title_full	Urinary lipocalin protein in a female rodent with correlation to phases in the estrous cycle: an experimental study accompanied by in silico analysis.
title_fullStr	Urinary lipocalin protein in a female rodent with correlation to phases in the estrous cycle: an experimental study accompanied by in silico analysis.
title_full_unstemmed	Urinary lipocalin protein in a female rodent with correlation to phases in the estrous cycle: an experimental study accompanied by in silico analysis.
title_sort	urinary lipocalin protein in a female rodent with correlation to phases in the estrous cycle: an experimental study accompanied by in silico analysis.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/4f9a60c313c345c799d4b02c30f43a97
work_keys_str_mv	AT subramanianmuthukumar urinarylipocalinproteininafemalerodentwithcorrelationtophasesintheestrouscycleanexperimentalstudyaccompaniedbyinsilicoanalysis AT durairajrajesh urinarylipocalinproteininafemalerodentwithcorrelationtophasesintheestrouscycleanexperimentalstudyaccompaniedbyinsilicoanalysis AT ganesansaibaba urinarylipocalinproteininafemalerodentwithcorrelationtophasesintheestrouscycleanexperimentalstudyaccompaniedbyinsilicoanalysis AT alagersamyalagesan urinarylipocalinproteininafemalerodentwithcorrelationtophasesintheestrouscycleanexperimentalstudyaccompaniedbyinsilicoanalysis AT rengasamylakhsminarayananrengarajan urinarylipocalinproteininafemalerodentwithcorrelationtophasesintheestrouscycleanexperimentalstudyaccompaniedbyinsilicoanalysis AT govindarajuarchunan urinarylipocalinproteininafemalerodentwithcorrelationtophasesintheestrouscycleanexperimentalstudyaccompaniedbyinsilicoanalysis
_version_	1718421020268822528

Urinary lipocalin protein in a female rodent with correlation to phases in the estrous cycle: an experimental study accompanied by in silico analysis.

Ejemplares similares