Production of minor ginsenosides by combining Stereum hirsutum and cellulase.

Minor ginsenosides (MGs) (include ginsenoside F2, Compound K, PPT, etc), which are generally not produced by ginseng plants naturally, are obtained by deglycosylation of major ginsenosides. However, the conventional processes used to produce deglycosylated ginsenosides focus on the use of intestinal...

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Autores principales:	Wenhua Yang, Jianli Zhou, Jean Damascene Harindintwali, Xiaobin Yu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4fa0eaa22fbe4f1f9a4a1d1f9aaf330e
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spelling	oai:doaj.org-article:4fa0eaa22fbe4f1f9a4a1d1f9aaf330e2021-12-02T20:18:34ZProduction of minor ginsenosides by combining Stereum hirsutum and cellulase.1932-620310.1371/journal.pone.0255899https://doaj.org/article/4fa0eaa22fbe4f1f9a4a1d1f9aaf330e2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255899https://doaj.org/toc/1932-6203Minor ginsenosides (MGs) (include ginsenoside F2, Compound K, PPT, etc), which are generally not produced by ginseng plants naturally, are obtained by deglycosylation of major ginsenosides. However, the conventional processes used to produce deglycosylated ginsenosides focus on the use of intestinal microorganisms for transformation. In this study, an edible and medicinal mushroom Stereum hirsutum JE0512 was screened from 161 β-glucosidase-producing soil microorganisms sourced from wild ginseng using the plate coloration method. Furthermore, JE0512 was used for the production of CK from ginseng extracts (GE) in solid-state fermentation (SSF) using 20 g corn bran as substrate, 4 g GE, and 20% inoculation volume, and the results showed that the highest CK content was 29.13 mg/g. After combining S. hirsutum JE0512 with cellulase (Aspergillus niger), the MGs (F2, CK, and PPT) content increased from 1.66 to 130.79 mg/g in the final products. Our results indicate that the Stereum genus has the potential to biotransform GE into CK and the combination of S. hirsutum JE0512 and cellulase could pave the way for the production of MGs from GE.Wenhua YangJianli ZhouJean Damascene HarindintwaliXiaobin YuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255899 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wenhua Yang Jianli Zhou Jean Damascene Harindintwali Xiaobin Yu Production of minor ginsenosides by combining Stereum hirsutum and cellulase.
description	Minor ginsenosides (MGs) (include ginsenoside F2, Compound K, PPT, etc), which are generally not produced by ginseng plants naturally, are obtained by deglycosylation of major ginsenosides. However, the conventional processes used to produce deglycosylated ginsenosides focus on the use of intestinal microorganisms for transformation. In this study, an edible and medicinal mushroom Stereum hirsutum JE0512 was screened from 161 β-glucosidase-producing soil microorganisms sourced from wild ginseng using the plate coloration method. Furthermore, JE0512 was used for the production of CK from ginseng extracts (GE) in solid-state fermentation (SSF) using 20 g corn bran as substrate, 4 g GE, and 20% inoculation volume, and the results showed that the highest CK content was 29.13 mg/g. After combining S. hirsutum JE0512 with cellulase (Aspergillus niger), the MGs (F2, CK, and PPT) content increased from 1.66 to 130.79 mg/g in the final products. Our results indicate that the Stereum genus has the potential to biotransform GE into CK and the combination of S. hirsutum JE0512 and cellulase could pave the way for the production of MGs from GE.
format	article
author	Wenhua Yang Jianli Zhou Jean Damascene Harindintwali Xiaobin Yu
author_facet	Wenhua Yang Jianli Zhou Jean Damascene Harindintwali Xiaobin Yu
author_sort	Wenhua Yang
title	Production of minor ginsenosides by combining Stereum hirsutum and cellulase.
title_short	Production of minor ginsenosides by combining Stereum hirsutum and cellulase.
title_full	Production of minor ginsenosides by combining Stereum hirsutum and cellulase.
title_fullStr	Production of minor ginsenosides by combining Stereum hirsutum and cellulase.
title_full_unstemmed	Production of minor ginsenosides by combining Stereum hirsutum and cellulase.
title_sort	production of minor ginsenosides by combining stereum hirsutum and cellulase.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/4fa0eaa22fbe4f1f9a4a1d1f9aaf330e
work_keys_str_mv	AT wenhuayang productionofminorginsenosidesbycombiningstereumhirsutumandcellulase AT jianlizhou productionofminorginsenosidesbycombiningstereumhirsutumandcellulase AT jeandamasceneharindintwali productionofminorginsenosidesbycombiningstereumhirsutumandcellulase AT xiaobinyu productionofminorginsenosidesbycombiningstereumhirsutumandcellulase
_version_	1718374283411980288

Production of minor ginsenosides by combining Stereum hirsutum and cellulase.

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