Unraveling the role of the CbrA histidine kinase in the signal transduction of the CbrAB two-component system in Pseudomonas putida

Abstract The histidine kinase CbrA of the CbrAB two-component system of Pseudomonas putida is a key element to recognise the activating signal and mediate auto- and trans-phosphorylation of the response element CbrB. CbrA is encoded by the gene cbrA which is located downstream of a putative open rea...

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Autores principales:	Elizabet Monteagudo-Cascales, Sofía M. García-Mauriño, Eduardo Santero, Inés Canosa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4fa56bc692f148dfb4ddfe9593335e52
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spelling	oai:doaj.org-article:4fa56bc692f148dfb4ddfe9593335e522021-12-02T15:09:20ZUnraveling the role of the CbrA histidine kinase in the signal transduction of the CbrAB two-component system in Pseudomonas putida10.1038/s41598-019-45554-92045-2322https://doaj.org/article/4fa56bc692f148dfb4ddfe9593335e522019-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-45554-9https://doaj.org/toc/2045-2322Abstract The histidine kinase CbrA of the CbrAB two-component system of Pseudomonas putida is a key element to recognise the activating signal and mediate auto- and trans-phosphorylation of the response element CbrB. CbrA is encoded by the gene cbrA which is located downstream of a putative open reading frame we have named cbrX. We describe the role of the CbrX product in the expression of CbrA and show there is translational coupling of the genes. We also explore the role of the transmembrane (TM) and PAS domains of CbrA in the signal recognition. A ΔcbrXA mutant lacking its TM domains is uncoupled in its growth in histidine and citrate as carbon sources, but its overexpression restores the ability to grow in such carbon sources. In these conditions ΔTM-CbrA is able to respond to carbon availability, thus suggesting an intracellular nature for the signal sensed.Elizabet Monteagudo-CascalesSofía M. García-MauriñoEduardo SanteroInés CanosaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-14 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elizabet Monteagudo-Cascales Sofía M. García-Mauriño Eduardo Santero Inés Canosa Unraveling the role of the CbrA histidine kinase in the signal transduction of the CbrAB two-component system in Pseudomonas putida
description	Abstract The histidine kinase CbrA of the CbrAB two-component system of Pseudomonas putida is a key element to recognise the activating signal and mediate auto- and trans-phosphorylation of the response element CbrB. CbrA is encoded by the gene cbrA which is located downstream of a putative open reading frame we have named cbrX. We describe the role of the CbrX product in the expression of CbrA and show there is translational coupling of the genes. We also explore the role of the transmembrane (TM) and PAS domains of CbrA in the signal recognition. A ΔcbrXA mutant lacking its TM domains is uncoupled in its growth in histidine and citrate as carbon sources, but its overexpression restores the ability to grow in such carbon sources. In these conditions ΔTM-CbrA is able to respond to carbon availability, thus suggesting an intracellular nature for the signal sensed.
format	article
author	Elizabet Monteagudo-Cascales Sofía M. García-Mauriño Eduardo Santero Inés Canosa
author_facet	Elizabet Monteagudo-Cascales Sofía M. García-Mauriño Eduardo Santero Inés Canosa
author_sort	Elizabet Monteagudo-Cascales
title	Unraveling the role of the CbrA histidine kinase in the signal transduction of the CbrAB two-component system in Pseudomonas putida
title_short	Unraveling the role of the CbrA histidine kinase in the signal transduction of the CbrAB two-component system in Pseudomonas putida
title_full	Unraveling the role of the CbrA histidine kinase in the signal transduction of the CbrAB two-component system in Pseudomonas putida
title_fullStr	Unraveling the role of the CbrA histidine kinase in the signal transduction of the CbrAB two-component system in Pseudomonas putida
title_full_unstemmed	Unraveling the role of the CbrA histidine kinase in the signal transduction of the CbrAB two-component system in Pseudomonas putida
title_sort	unraveling the role of the cbra histidine kinase in the signal transduction of the cbrab two-component system in pseudomonas putida
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/4fa56bc692f148dfb4ddfe9593335e52
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Unraveling the role of the CbrA histidine kinase in the signal transduction of the CbrAB two-component system in Pseudomonas putida

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