Atopy in Kashmir-validation from a case control study with respect to IgE and Interleukin genes

Abstract Objectives Increased levels of serum Immunoglobulin-E (IgE) and different genetic variants of cytokines are common biochemical manifestation in Allergy. The current study was aimed to study the association of IgE and different variants of Interleukin-4 (IL-4), and Interleukin-13 (IL-13) gen...

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Autores principales:	Taha Ashraf Qurashi, Aaliya Shah, Gulzar Ahmad Bhat, Mosin Saleem Khan, Roohi Rasool, Syed Mudassar
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	IgE Interleukin gene variants Atopy Case control study Kashmir Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/4fa9acb4fc1f4347873cd5eca5006be9
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spelling	oai:doaj.org-article:4fa9acb4fc1f4347873cd5eca5006be92021-11-28T12:36:16ZAtopy in Kashmir-validation from a case control study with respect to IgE and Interleukin genes10.1186/s13223-021-00623-51710-1492https://doaj.org/article/4fa9acb4fc1f4347873cd5eca5006be92021-11-01T00:00:00Zhttps://doi.org/10.1186/s13223-021-00623-5https://doaj.org/toc/1710-1492Abstract Objectives Increased levels of serum Immunoglobulin-E (IgE) and different genetic variants of cytokines are common biochemical manifestation in Allergy. The current study was aimed to study the association of IgE and different variants of Interleukin-4 (IL-4), and Interleukin-13 (IL-13) genes with different kind of allergies. Methods A pre-tested questionnaire was used to collect all the dietary, life style and clinical details by a trained staff. A blood sample of 2 ml each was collected in coagulated and anti-coagulated vials. DNA and serum samples were extracted and stored until further use. Serum IgE were estimated by ELISA while as the genotypic analysis was done by PCR–RFLP methods. Results Statistically a significant difference of serum IgE levels were observed among cases and controls (P < 0.05). The observed significant difference of serum IgE levels were retained among subjects who also harboured variant genotypes of IL-4 and IL-13 genes (P < 0.05). Additionally, the above genetic variants significantly modified the risk of allergy when stratification was done based on various clinical characteristics. Conclusion Our study suggests that increased IgE levels and in association with variant forms of IL-4 and IL-13 genes are significantly associated with different types of allergies in study population.Taha Ashraf QurashiAaliya ShahGulzar Ahmad BhatMosin Saleem KhanRoohi RasoolSyed MudassarBMCarticleIgEInterleukin gene variantsAtopyCase control studyKashmirImmunologic diseases. AllergyRC581-607ENAllergy, Asthma & Clinical Immunology, Vol 17, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	IgE Interleukin gene variants Atopy Case control study Kashmir Immunologic diseases. Allergy RC581-607
spellingShingle	IgE Interleukin gene variants Atopy Case control study Kashmir Immunologic diseases. Allergy RC581-607 Taha Ashraf Qurashi Aaliya Shah Gulzar Ahmad Bhat Mosin Saleem Khan Roohi Rasool Syed Mudassar Atopy in Kashmir-validation from a case control study with respect to IgE and Interleukin genes
description	Abstract Objectives Increased levels of serum Immunoglobulin-E (IgE) and different genetic variants of cytokines are common biochemical manifestation in Allergy. The current study was aimed to study the association of IgE and different variants of Interleukin-4 (IL-4), and Interleukin-13 (IL-13) genes with different kind of allergies. Methods A pre-tested questionnaire was used to collect all the dietary, life style and clinical details by a trained staff. A blood sample of 2 ml each was collected in coagulated and anti-coagulated vials. DNA and serum samples were extracted and stored until further use. Serum IgE were estimated by ELISA while as the genotypic analysis was done by PCR–RFLP methods. Results Statistically a significant difference of serum IgE levels were observed among cases and controls (P < 0.05). The observed significant difference of serum IgE levels were retained among subjects who also harboured variant genotypes of IL-4 and IL-13 genes (P < 0.05). Additionally, the above genetic variants significantly modified the risk of allergy when stratification was done based on various clinical characteristics. Conclusion Our study suggests that increased IgE levels and in association with variant forms of IL-4 and IL-13 genes are significantly associated with different types of allergies in study population.
format	article
author	Taha Ashraf Qurashi Aaliya Shah Gulzar Ahmad Bhat Mosin Saleem Khan Roohi Rasool Syed Mudassar
author_facet	Taha Ashraf Qurashi Aaliya Shah Gulzar Ahmad Bhat Mosin Saleem Khan Roohi Rasool Syed Mudassar
author_sort	Taha Ashraf Qurashi
title	Atopy in Kashmir-validation from a case control study with respect to IgE and Interleukin genes
title_short	Atopy in Kashmir-validation from a case control study with respect to IgE and Interleukin genes
title_full	Atopy in Kashmir-validation from a case control study with respect to IgE and Interleukin genes
title_fullStr	Atopy in Kashmir-validation from a case control study with respect to IgE and Interleukin genes
title_full_unstemmed	Atopy in Kashmir-validation from a case control study with respect to IgE and Interleukin genes
title_sort	atopy in kashmir-validation from a case control study with respect to ige and interleukin genes
publisher	BMC
publishDate	2021
url	https://doaj.org/article/4fa9acb4fc1f4347873cd5eca5006be9
work_keys_str_mv	AT tahaashrafqurashi atopyinkashmirvalidationfromacasecontrolstudywithrespecttoigeandinterleukingenes AT aaliyashah atopyinkashmirvalidationfromacasecontrolstudywithrespecttoigeandinterleukingenes AT gulzarahmadbhat atopyinkashmirvalidationfromacasecontrolstudywithrespecttoigeandinterleukingenes AT mosinsaleemkhan atopyinkashmirvalidationfromacasecontrolstudywithrespecttoigeandinterleukingenes AT roohirasool atopyinkashmirvalidationfromacasecontrolstudywithrespecttoigeandinterleukingenes AT syedmudassar atopyinkashmirvalidationfromacasecontrolstudywithrespecttoigeandinterleukingenes
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Atopy in Kashmir-validation from a case control study with respect to IgE and Interleukin genes

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