Modeling of stringent-response reflects nutrient stress induced growth impairment and essential amino acids in different Staphylococcus aureus mutants

Abstract Stapylococcus aureus colonises the nose of healthy individuals but can also cause a wide range of infections. Amino acid (AA) synthesis and their availability is crucial to adapt to conditions encountered in vivo. Most S. aureus genomes comprise all genes required for AA biosynthesis. Never...

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Auteurs principaux: Christof Audretsch, Fabio Gratani, Christiane Wolz, Thomas Dandekar
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Langue:EN
Publié: Nature Portfolio 2021
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spelling oai:doaj.org-article:4fae153651f442bdae3c6c2406c2e7992021-12-02T16:51:31ZModeling of stringent-response reflects nutrient stress induced growth impairment and essential amino acids in different Staphylococcus aureus mutants10.1038/s41598-021-88646-12045-2322https://doaj.org/article/4fae153651f442bdae3c6c2406c2e7992021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88646-1https://doaj.org/toc/2045-2322Abstract Stapylococcus aureus colonises the nose of healthy individuals but can also cause a wide range of infections. Amino acid (AA) synthesis and their availability is crucial to adapt to conditions encountered in vivo. Most S. aureus genomes comprise all genes required for AA biosynthesis. Nevertheless, different strains require specific sets of AAs for growth. In this study we show that regulation inactivates pathways under certain conditions which result in these observed auxotrophies. We analyzed in vitro and modeled in silico in a Boolean semiquantitative model (195 nodes, 320 edges) the regulatory impact of stringent response (SR) on AA requirement in S. aureus HG001 (wild-type) and in mutant strains lacking the metabolic regulators RSH, CodY and CcpA, respectively. Growth in medium lacking single AAs was analyzed. Results correlated qualitatively to the in silico predictions of the final model in 92% and quantitatively in 81%. Remaining gaps in our knowledge are evaluated and discussed. This in silico model is made fully available and explains how integration of different inputs is achieved in SR and AA metabolism of S. aureus. The in vitro data and in silico modeling stress the role of SR and central regulators such as CodY for AA metabolisms in S. aureus.Christof AudretschFabio GrataniChristiane WolzThomas DandekarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Christof Audretsch
Fabio Gratani
Christiane Wolz
Thomas Dandekar
Modeling of stringent-response reflects nutrient stress induced growth impairment and essential amino acids in different Staphylococcus aureus mutants
description Abstract Stapylococcus aureus colonises the nose of healthy individuals but can also cause a wide range of infections. Amino acid (AA) synthesis and their availability is crucial to adapt to conditions encountered in vivo. Most S. aureus genomes comprise all genes required for AA biosynthesis. Nevertheless, different strains require specific sets of AAs for growth. In this study we show that regulation inactivates pathways under certain conditions which result in these observed auxotrophies. We analyzed in vitro and modeled in silico in a Boolean semiquantitative model (195 nodes, 320 edges) the regulatory impact of stringent response (SR) on AA requirement in S. aureus HG001 (wild-type) and in mutant strains lacking the metabolic regulators RSH, CodY and CcpA, respectively. Growth in medium lacking single AAs was analyzed. Results correlated qualitatively to the in silico predictions of the final model in 92% and quantitatively in 81%. Remaining gaps in our knowledge are evaluated and discussed. This in silico model is made fully available and explains how integration of different inputs is achieved in SR and AA metabolism of S. aureus. The in vitro data and in silico modeling stress the role of SR and central regulators such as CodY for AA metabolisms in S. aureus.
format article
author Christof Audretsch
Fabio Gratani
Christiane Wolz
Thomas Dandekar
author_facet Christof Audretsch
Fabio Gratani
Christiane Wolz
Thomas Dandekar
author_sort Christof Audretsch
title Modeling of stringent-response reflects nutrient stress induced growth impairment and essential amino acids in different Staphylococcus aureus mutants
title_short Modeling of stringent-response reflects nutrient stress induced growth impairment and essential amino acids in different Staphylococcus aureus mutants
title_full Modeling of stringent-response reflects nutrient stress induced growth impairment and essential amino acids in different Staphylococcus aureus mutants
title_fullStr Modeling of stringent-response reflects nutrient stress induced growth impairment and essential amino acids in different Staphylococcus aureus mutants
title_full_unstemmed Modeling of stringent-response reflects nutrient stress induced growth impairment and essential amino acids in different Staphylococcus aureus mutants
title_sort modeling of stringent-response reflects nutrient stress induced growth impairment and essential amino acids in different staphylococcus aureus mutants
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/4fae153651f442bdae3c6c2406c2e799
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AT fabiogratani modelingofstringentresponsereflectsnutrientstressinducedgrowthimpairmentandessentialaminoacidsindifferentstaphylococcusaureusmutants
AT christianewolz modelingofstringentresponsereflectsnutrientstressinducedgrowthimpairmentandessentialaminoacidsindifferentstaphylococcusaureusmutants
AT thomasdandekar modelingofstringentresponsereflectsnutrientstressinducedgrowthimpairmentandessentialaminoacidsindifferentstaphylococcusaureusmutants
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