Chromatic intervention and biocompatibility assay for biosurfactant derived from Balanites aegyptiaca (L.) Del

Abstract Extraction of biosurfactants from plants is advantageous than from microbes. The properties and robustness of biosurfactant derived from the mesocarp of Balanites aegyptiaca have been reported. However, the dark brown property of biosurfactant and lack of knowledge of its biocompatibility l...

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Autores principales:	Vishal Panchariya, Vishal Bhati, Harishkumar Madhyastha, Radha Madhyastha, Jagdish Prasad, Priyal Sharma, Paras Sharma, Harish, Mahesh Kumar Saini, Vishnu D. Rajput, Yuichi Nakajima, S. L. Kothari, Vinod Singh Gour
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4fb5e3de479d4bd2862be4408950707f
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spelling	oai:doaj.org-article:4fb5e3de479d4bd2862be4408950707f2021-12-02T14:03:45ZChromatic intervention and biocompatibility assay for biosurfactant derived from Balanites aegyptiaca (L.) Del10.1038/s41598-021-83573-72045-2322https://doaj.org/article/4fb5e3de479d4bd2862be4408950707f2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83573-7https://doaj.org/toc/2045-2322Abstract Extraction of biosurfactants from plants is advantageous than from microbes. The properties and robustness of biosurfactant derived from the mesocarp of Balanites aegyptiaca have been reported. However, the dark brown property of biosurfactant and lack of knowledge of its biocompatibility limits its scope. In the present work, the decolorization protocol for this biosurfactant was optimized using hydrogen peroxide. The hemolytic potential and biocompatibility based on cell toxicity and proliferation were also investigated. This study is the first report on the decolorization and toxicity assay of this biosurfactant. For decolorization of biosurfactant, 34 full factorial design was used, and the data were subjected to ANOVA. Results indicate that 1.5% of hydrogen peroxide can decolorize the biosurfactant most efficiently at 40 °C in 70 min at pH 7. Mitochondrial reductase (MTT) and reactive oxygen species (ROS) assays on M5S mouse skin fibroblast cells revealed that decolorized biosurfactant up to 50 µg/mL for 6 h had no significant toxic effect. Hemolysis assay showed ~ 2.5% hemolysis of human RBCs, indicating the nontoxic effect of this biosurfactant. The present work established a decolorization protocol making the biosurfactant chromatically acceptable. Biocompatibility assays confirm its safer use as observed by experiments on M5S skin fibroblast cells under in vitro conditions.Vishal PanchariyaVishal BhatiHarishkumar MadhyasthaRadha MadhyasthaJagdish PrasadPriyal SharmaParas SharmaHarishMahesh Kumar SainiVishnu D. RajputYuichi NakajimaS. L. KothariVinod Singh GourNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vishal Panchariya Vishal Bhati Harishkumar Madhyastha Radha Madhyastha Jagdish Prasad Priyal Sharma Paras Sharma Harish Mahesh Kumar Saini Vishnu D. Rajput Yuichi Nakajima S. L. Kothari Vinod Singh Gour Chromatic intervention and biocompatibility assay for biosurfactant derived from Balanites aegyptiaca (L.) Del
description	Abstract Extraction of biosurfactants from plants is advantageous than from microbes. The properties and robustness of biosurfactant derived from the mesocarp of Balanites aegyptiaca have been reported. However, the dark brown property of biosurfactant and lack of knowledge of its biocompatibility limits its scope. In the present work, the decolorization protocol for this biosurfactant was optimized using hydrogen peroxide. The hemolytic potential and biocompatibility based on cell toxicity and proliferation were also investigated. This study is the first report on the decolorization and toxicity assay of this biosurfactant. For decolorization of biosurfactant, 34 full factorial design was used, and the data were subjected to ANOVA. Results indicate that 1.5% of hydrogen peroxide can decolorize the biosurfactant most efficiently at 40 °C in 70 min at pH 7. Mitochondrial reductase (MTT) and reactive oxygen species (ROS) assays on M5S mouse skin fibroblast cells revealed that decolorized biosurfactant up to 50 µg/mL for 6 h had no significant toxic effect. Hemolysis assay showed ~ 2.5% hemolysis of human RBCs, indicating the nontoxic effect of this biosurfactant. The present work established a decolorization protocol making the biosurfactant chromatically acceptable. Biocompatibility assays confirm its safer use as observed by experiments on M5S skin fibroblast cells under in vitro conditions.
format	article
author	Vishal Panchariya Vishal Bhati Harishkumar Madhyastha Radha Madhyastha Jagdish Prasad Priyal Sharma Paras Sharma Harish Mahesh Kumar Saini Vishnu D. Rajput Yuichi Nakajima S. L. Kothari Vinod Singh Gour
author_facet	Vishal Panchariya Vishal Bhati Harishkumar Madhyastha Radha Madhyastha Jagdish Prasad Priyal Sharma Paras Sharma Harish Mahesh Kumar Saini Vishnu D. Rajput Yuichi Nakajima S. L. Kothari Vinod Singh Gour
author_sort	Vishal Panchariya
title	Chromatic intervention and biocompatibility assay for biosurfactant derived from Balanites aegyptiaca (L.) Del
title_short	Chromatic intervention and biocompatibility assay for biosurfactant derived from Balanites aegyptiaca (L.) Del
title_full	Chromatic intervention and biocompatibility assay for biosurfactant derived from Balanites aegyptiaca (L.) Del
title_fullStr	Chromatic intervention and biocompatibility assay for biosurfactant derived from Balanites aegyptiaca (L.) Del
title_full_unstemmed	Chromatic intervention and biocompatibility assay for biosurfactant derived from Balanites aegyptiaca (L.) Del
title_sort	chromatic intervention and biocompatibility assay for biosurfactant derived from balanites aegyptiaca (l.) del
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4fb5e3de479d4bd2862be4408950707f
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Chromatic intervention and biocompatibility assay for biosurfactant derived from Balanites aegyptiaca (L.) Del

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