Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes

Abstract Electroporation of in-vitro cultured cells is widely used in biological and medical areas to deliver molecules of interest inside cells. Since very high electric fields are required to electroporate the plasma membrane, depending on the geometry of the electrodes the required voltages can b...

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Autores principales:	Valeria Caprettini, Andrea Cerea, Giovanni Melle, Laura Lovato, Rosario Capozza, Jian-An Huang, Francesco Tantussi, Michele Dipalo, Francesco De Angelis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4fb8d043f6cb46fd948d5cb1b60ac029
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spelling	oai:doaj.org-article:4fb8d043f6cb46fd948d5cb1b60ac0292021-12-02T15:06:21ZSoft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes10.1038/s41598-017-08886-y2045-2322https://doaj.org/article/4fb8d043f6cb46fd948d5cb1b60ac0292017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08886-yhttps://doaj.org/toc/2045-2322Abstract Electroporation of in-vitro cultured cells is widely used in biological and medical areas to deliver molecules of interest inside cells. Since very high electric fields are required to electroporate the plasma membrane, depending on the geometry of the electrodes the required voltages can be very high and often critical to cell viability. Furthermore, in traditional electroporation configuration based on planar electrodes there is no a priori certain feedback about which cell has been targeted and delivered and the addition of fluorophores may be needed to gain this information. In this study we present a nanofabricated platform able to perform intracellular delivery of membrane-impermeable molecules by opening transient nanopores into the lipid membrane of adherent cells with high spatial precision and with the application of low voltages (1.5–2 V). This result is obtained by exploiting the tight seal that the cells present with 3D fluidic hollow gold-coated nanostructures that act as nanochannels and nanoelectrodes at the same time. The final soft-electroporation platform provides an accessible approach for controlled and selective drug delivery on ordered arrangements of cells.Valeria CaprettiniAndrea CereaGiovanni MelleLaura LovatoRosario CapozzaJian-An HuangFrancesco TantussiMichele DipaloFrancesco De AngelisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Valeria Caprettini Andrea Cerea Giovanni Melle Laura Lovato Rosario Capozza Jian-An Huang Francesco Tantussi Michele Dipalo Francesco De Angelis Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes
description	Abstract Electroporation of in-vitro cultured cells is widely used in biological and medical areas to deliver molecules of interest inside cells. Since very high electric fields are required to electroporate the plasma membrane, depending on the geometry of the electrodes the required voltages can be very high and often critical to cell viability. Furthermore, in traditional electroporation configuration based on planar electrodes there is no a priori certain feedback about which cell has been targeted and delivered and the addition of fluorophores may be needed to gain this information. In this study we present a nanofabricated platform able to perform intracellular delivery of membrane-impermeable molecules by opening transient nanopores into the lipid membrane of adherent cells with high spatial precision and with the application of low voltages (1.5–2 V). This result is obtained by exploiting the tight seal that the cells present with 3D fluidic hollow gold-coated nanostructures that act as nanochannels and nanoelectrodes at the same time. The final soft-electroporation platform provides an accessible approach for controlled and selective drug delivery on ordered arrangements of cells.
format	article
author	Valeria Caprettini Andrea Cerea Giovanni Melle Laura Lovato Rosario Capozza Jian-An Huang Francesco Tantussi Michele Dipalo Francesco De Angelis
author_facet	Valeria Caprettini Andrea Cerea Giovanni Melle Laura Lovato Rosario Capozza Jian-An Huang Francesco Tantussi Michele Dipalo Francesco De Angelis
author_sort	Valeria Caprettini
title	Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes
title_short	Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes
title_full	Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes
title_fullStr	Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes
title_full_unstemmed	Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes
title_sort	soft electroporation for delivering molecules into tightly adherent mammalian cells through 3d hollow nanoelectrodes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4fb8d043f6cb46fd948d5cb1b60ac029
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Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes

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