CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome

CRISPR/Cas9 gene editing has been used to generate mutations in several mouse genes. Here, the authors show that targeting events using single guide RNAs cause large deletions at 17 sites in the mouse genome, suggesting that careful genotyping is needed and sequential targeting may avoid such deleti...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ha Youn Shin, Chaochen Wang, Hye Kyung Lee, Kyung Hyun Yoo, Xianke Zeng, Tyler Kuhns, Chul Min Yang, Teresa Mohr, Chengyu Liu, Lothar Hennighausen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4fbfd858eb664ea8891c7763c979d634
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4fbfd858eb664ea8891c7763c979d634
record_format	dspace
spelling	oai:doaj.org-article:4fbfd858eb664ea8891c7763c979d6342021-12-02T14:40:55ZCRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome10.1038/ncomms154642041-1723https://doaj.org/article/4fbfd858eb664ea8891c7763c979d6342017-05-01T00:00:00Zhttps://doi.org/10.1038/ncomms15464https://doaj.org/toc/2041-1723CRISPR/Cas9 gene editing has been used to generate mutations in several mouse genes. Here, the authors show that targeting events using single guide RNAs cause large deletions at 17 sites in the mouse genome, suggesting that careful genotyping is needed and sequential targeting may avoid such deletions.Ha Youn ShinChaochen WangHye Kyung LeeKyung Hyun YooXianke ZengTyler KuhnsChul Min YangTeresa MohrChengyu LiuLothar HennighausenNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Ha Youn Shin Chaochen Wang Hye Kyung Lee Kyung Hyun Yoo Xianke Zeng Tyler Kuhns Chul Min Yang Teresa Mohr Chengyu Liu Lothar Hennighausen CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome
description	CRISPR/Cas9 gene editing has been used to generate mutations in several mouse genes. Here, the authors show that targeting events using single guide RNAs cause large deletions at 17 sites in the mouse genome, suggesting that careful genotyping is needed and sequential targeting may avoid such deletions.
format	article
author	Ha Youn Shin Chaochen Wang Hye Kyung Lee Kyung Hyun Yoo Xianke Zeng Tyler Kuhns Chul Min Yang Teresa Mohr Chengyu Liu Lothar Hennighausen
author_facet	Ha Youn Shin Chaochen Wang Hye Kyung Lee Kyung Hyun Yoo Xianke Zeng Tyler Kuhns Chul Min Yang Teresa Mohr Chengyu Liu Lothar Hennighausen
author_sort	Ha Youn Shin
title	CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome
title_short	CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome
title_full	CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome
title_fullStr	CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome
title_full_unstemmed	CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome
title_sort	crispr/cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4fbfd858eb664ea8891c7763c979d634
work_keys_str_mv	AT hayounshin crisprcas9targetingeventscausecomplexdeletionsandinsertionsat17sitesinthemousegenome AT chaochenwang crisprcas9targetingeventscausecomplexdeletionsandinsertionsat17sitesinthemousegenome AT hyekyunglee crisprcas9targetingeventscausecomplexdeletionsandinsertionsat17sitesinthemousegenome AT kyunghyunyoo crisprcas9targetingeventscausecomplexdeletionsandinsertionsat17sitesinthemousegenome AT xiankezeng crisprcas9targetingeventscausecomplexdeletionsandinsertionsat17sitesinthemousegenome AT tylerkuhns crisprcas9targetingeventscausecomplexdeletionsandinsertionsat17sitesinthemousegenome AT chulminyang crisprcas9targetingeventscausecomplexdeletionsandinsertionsat17sitesinthemousegenome AT teresamohr crisprcas9targetingeventscausecomplexdeletionsandinsertionsat17sitesinthemousegenome AT chengyuliu crisprcas9targetingeventscausecomplexdeletionsandinsertionsat17sitesinthemousegenome AT lotharhennighausen crisprcas9targetingeventscausecomplexdeletionsandinsertionsat17sitesinthemousegenome
_version_	1718390109391290368

CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome

Ejemplares similares