Evaluating the Presence and Contents of Phytochemicals in Honey Samples: Phenolic Compounds as Indicators to Identify Their Botanical Origin

Honey is a natural product well known for its beneficial properties. It contains phytochemicals, a wide class of nutraceuticals found in plants, including compounds with highly demonstrated antimicrobial and antioxidant capacities as phenolic compounds and flavonoids. The main goal of this work is t...

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Autores principales:	Lua Vazquez, Daniel Armada, Maria Celeiro, Thierry Dagnac, Maria Llompart
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	honey polyphenols phenolic profile total phenolic content antioxidant activity liquid chromatography Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/4fc252d85a0a4953af5be6703df4c3b6
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spelling	oai:doaj.org-article:4fc252d85a0a4953af5be6703df4c3b62021-11-25T17:33:29ZEvaluating the Presence and Contents of Phytochemicals in Honey Samples: Phenolic Compounds as Indicators to Identify Their Botanical Origin10.3390/foods101126162304-8158https://doaj.org/article/4fc252d85a0a4953af5be6703df4c3b62021-10-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2616https://doaj.org/toc/2304-8158Honey is a natural product well known for its beneficial properties. It contains phytochemicals, a wide class of nutraceuticals found in plants, including compounds with highly demonstrated antimicrobial and antioxidant capacities as phenolic compounds and flavonoids. The main goal of this work is the development of a miniaturized and environmentally friendly methodology to obtain the phenolic profile of Galician honeys (Northwest Spain) from different varieties such as honeydew, chestnut, eucalyptus, heather, blackberry and multi-floral. The total phenolic content (TPC) and antioxidant activity (AA) were also evaluated. As regards sample preparation, miniaturized vortex (VE) and ultrasound assisted extraction (UAE) employing aqueous-based solvents were performed. Individual quantification of 41 target phenolic compounds was carried out by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results revealed the presence of 25 phenolic compounds in the 91 analyzed samples, reaching concentrations up to 252 µg g<sup>−1</sup>. Statistical tools such as analysis of variance (ANOVA) and principal component analysis (PCA) were employed to obtain models that allowed classifying the different honeys according to their botanical origin. Obtained results, based on TPC, AA and ∑phenolic compounds showed that significant differences appeared depending on the honey variety, being several of the identified phenol compounds being responsible of the main differentiation.Lua VazquezDaniel ArmadaMaria CeleiroThierry DagnacMaria LlompartMDPI AGarticlehoneypolyphenolsphenolic profiletotal phenolic contentantioxidant activityliquid chromatographyChemical technologyTP1-1185ENFoods, Vol 10, Iss 2616, p 2616 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	honey polyphenols phenolic profile total phenolic content antioxidant activity liquid chromatography Chemical technology TP1-1185
spellingShingle	honey polyphenols phenolic profile total phenolic content antioxidant activity liquid chromatography Chemical technology TP1-1185 Lua Vazquez Daniel Armada Maria Celeiro Thierry Dagnac Maria Llompart Evaluating the Presence and Contents of Phytochemicals in Honey Samples: Phenolic Compounds as Indicators to Identify Their Botanical Origin
description	Honey is a natural product well known for its beneficial properties. It contains phytochemicals, a wide class of nutraceuticals found in plants, including compounds with highly demonstrated antimicrobial and antioxidant capacities as phenolic compounds and flavonoids. The main goal of this work is the development of a miniaturized and environmentally friendly methodology to obtain the phenolic profile of Galician honeys (Northwest Spain) from different varieties such as honeydew, chestnut, eucalyptus, heather, blackberry and multi-floral. The total phenolic content (TPC) and antioxidant activity (AA) were also evaluated. As regards sample preparation, miniaturized vortex (VE) and ultrasound assisted extraction (UAE) employing aqueous-based solvents were performed. Individual quantification of 41 target phenolic compounds was carried out by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results revealed the presence of 25 phenolic compounds in the 91 analyzed samples, reaching concentrations up to 252 µg g<sup>−1</sup>. Statistical tools such as analysis of variance (ANOVA) and principal component analysis (PCA) were employed to obtain models that allowed classifying the different honeys according to their botanical origin. Obtained results, based on TPC, AA and ∑phenolic compounds showed that significant differences appeared depending on the honey variety, being several of the identified phenol compounds being responsible of the main differentiation.
format	article
author	Lua Vazquez Daniel Armada Maria Celeiro Thierry Dagnac Maria Llompart
author_facet	Lua Vazquez Daniel Armada Maria Celeiro Thierry Dagnac Maria Llompart
author_sort	Lua Vazquez
title	Evaluating the Presence and Contents of Phytochemicals in Honey Samples: Phenolic Compounds as Indicators to Identify Their Botanical Origin
title_short	Evaluating the Presence and Contents of Phytochemicals in Honey Samples: Phenolic Compounds as Indicators to Identify Their Botanical Origin
title_full	Evaluating the Presence and Contents of Phytochemicals in Honey Samples: Phenolic Compounds as Indicators to Identify Their Botanical Origin
title_fullStr	Evaluating the Presence and Contents of Phytochemicals in Honey Samples: Phenolic Compounds as Indicators to Identify Their Botanical Origin
title_full_unstemmed	Evaluating the Presence and Contents of Phytochemicals in Honey Samples: Phenolic Compounds as Indicators to Identify Their Botanical Origin
title_sort	evaluating the presence and contents of phytochemicals in honey samples: phenolic compounds as indicators to identify their botanical origin
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4fc252d85a0a4953af5be6703df4c3b6
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Evaluating the Presence and Contents of Phytochemicals in Honey Samples: Phenolic Compounds as Indicators to Identify Their Botanical Origin

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