Expression of Genes Located on the Incompatibility Group FIB Plasmids at Transcription and Protein Levels in Iron-Modified Growth Conditions

Salmonella enterica strains often harbor plasmids representing several incompatibility groups (Inc) including IncFIB, which have been previously associated with carrying antimicrobial resistance and virulence associated genes. To better understand the distribution of virulence genes on IncFIB plasmi...

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Autores principales:	Carter N. Abbott, Monique Felix, Steven L. Foley, Bijay K. Khajanchi
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Salmonella enterica IncFIB plasmid iron acquisition systems IutA LBID LBIR Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4fcb088dd30441fc9b4be30378e65902
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spelling	oai:doaj.org-article:4fcb088dd30441fc9b4be30378e659022021-11-05T14:51:20ZExpression of Genes Located on the Incompatibility Group FIB Plasmids at Transcription and Protein Levels in Iron-Modified Growth Conditions1664-302X10.3389/fmicb.2021.729275https://doaj.org/article/4fcb088dd30441fc9b4be30378e659022021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.729275/fullhttps://doaj.org/toc/1664-302XSalmonella enterica strains often harbor plasmids representing several incompatibility groups (Inc) including IncFIB, which have been previously associated with carrying antimicrobial resistance and virulence associated genes. To better understand the distribution of virulence genes on IncFIB plasmids, we analyzed 37 complete whole genome and plasmid sequences of different S. enterica isolates from multiple serovars. Many of the sequences analyzed carried multiple virulence-associated genes, including those associated with iron acquisition systems; thus we aimed to determine how iron-rich (IR) and various iron-depleted (ID) conditions affected the transcription of iron acquisition and virulence genes including sitA, iutA, iucA, and enolase at different time intervals. sitA, iutA, and enolase from S. enterica that were grown in Luria-Bertani broth (LB) ID (LBID) conditions were substantially upregulated when compared to LBIR conditions. For both S. enterica strains that were grown at various LBID conditions, addition of 200 μM bipyridyl in the growth medium yielded the highest transcription for all four genes, followed by the 100 μM concentration. An antibody using a peptide targeting aerobactin receptor gene iutA encoded by IncFIB was generated and used to examine the protein expression in the wild-type, recipient, and transconjugant strain in LB, LBID, and LBIR growth conditions using Western blot analyses. A 70 KDa protein band was detected in the wild-type and transconjugant that carried the IncFIB plasmid, while this band was not detected in the recipient strain that lacked this plasmid.Carter N. AbbottMonique FelixSteven L. FoleyBijay K. KhajanchiFrontiers Media S.A.articleSalmonella entericaIncFIB plasmidiron acquisition systemsIutALBIDLBIRMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Salmonella enterica IncFIB plasmid iron acquisition systems IutA LBID LBIR Microbiology QR1-502
spellingShingle	Salmonella enterica IncFIB plasmid iron acquisition systems IutA LBID LBIR Microbiology QR1-502 Carter N. Abbott Monique Felix Steven L. Foley Bijay K. Khajanchi Expression of Genes Located on the Incompatibility Group FIB Plasmids at Transcription and Protein Levels in Iron-Modified Growth Conditions
description	Salmonella enterica strains often harbor plasmids representing several incompatibility groups (Inc) including IncFIB, which have been previously associated with carrying antimicrobial resistance and virulence associated genes. To better understand the distribution of virulence genes on IncFIB plasmids, we analyzed 37 complete whole genome and plasmid sequences of different S. enterica isolates from multiple serovars. Many of the sequences analyzed carried multiple virulence-associated genes, including those associated with iron acquisition systems; thus we aimed to determine how iron-rich (IR) and various iron-depleted (ID) conditions affected the transcription of iron acquisition and virulence genes including sitA, iutA, iucA, and enolase at different time intervals. sitA, iutA, and enolase from S. enterica that were grown in Luria-Bertani broth (LB) ID (LBID) conditions were substantially upregulated when compared to LBIR conditions. For both S. enterica strains that were grown at various LBID conditions, addition of 200 μM bipyridyl in the growth medium yielded the highest transcription for all four genes, followed by the 100 μM concentration. An antibody using a peptide targeting aerobactin receptor gene iutA encoded by IncFIB was generated and used to examine the protein expression in the wild-type, recipient, and transconjugant strain in LB, LBID, and LBIR growth conditions using Western blot analyses. A 70 KDa protein band was detected in the wild-type and transconjugant that carried the IncFIB plasmid, while this band was not detected in the recipient strain that lacked this plasmid.
format	article
author	Carter N. Abbott Monique Felix Steven L. Foley Bijay K. Khajanchi
author_facet	Carter N. Abbott Monique Felix Steven L. Foley Bijay K. Khajanchi
author_sort	Carter N. Abbott
title	Expression of Genes Located on the Incompatibility Group FIB Plasmids at Transcription and Protein Levels in Iron-Modified Growth Conditions
title_short	Expression of Genes Located on the Incompatibility Group FIB Plasmids at Transcription and Protein Levels in Iron-Modified Growth Conditions
title_full	Expression of Genes Located on the Incompatibility Group FIB Plasmids at Transcription and Protein Levels in Iron-Modified Growth Conditions
title_fullStr	Expression of Genes Located on the Incompatibility Group FIB Plasmids at Transcription and Protein Levels in Iron-Modified Growth Conditions
title_full_unstemmed	Expression of Genes Located on the Incompatibility Group FIB Plasmids at Transcription and Protein Levels in Iron-Modified Growth Conditions
title_sort	expression of genes located on the incompatibility group fib plasmids at transcription and protein levels in iron-modified growth conditions
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/4fcb088dd30441fc9b4be30378e65902
work_keys_str_mv	AT carternabbott expressionofgeneslocatedontheincompatibilitygroupfibplasmidsattranscriptionandproteinlevelsinironmodifiedgrowthconditions AT moniquefelix expressionofgeneslocatedontheincompatibilitygroupfibplasmidsattranscriptionandproteinlevelsinironmodifiedgrowthconditions AT stevenlfoley expressionofgeneslocatedontheincompatibilitygroupfibplasmidsattranscriptionandproteinlevelsinironmodifiedgrowthconditions AT bijaykkhajanchi expressionofgeneslocatedontheincompatibilitygroupfibplasmidsattranscriptionandproteinlevelsinironmodifiedgrowthconditions
_version_	1718444225405648896

Expression of Genes Located on the Incompatibility Group FIB Plasmids at Transcription and Protein Levels in Iron-Modified Growth Conditions

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