Analysis on Drug-Resistance-Associated Mutations among Multidrug-Resistant <i>Mycobacterium tuberculosis</i> Isolates in China

Analysis on Drug-Resistance-Associated Mutations among Multidrug-Resistant <i>Mycobacterium tuberculosis</i> Isolates in China

As the causative bacteria of tuberculosis, <i>Mycobacterium</i><i>tuberculosis (M. tb</i><i>)</i> is aggravated by the emergence of its multidrug-resistant isolates in China. Mutations of six of the most frequently reported resistant genes (<i>rpoB, katG, in...

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Autores principales: Hongbing Jia, Yuhui Xu, Zhaogang Sun
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
<i>Mycobacterium tuberculosis</i>
drug-susceptibility testing
multidrug resistance
mutation
Therapeutics. Pharmacology
RM1-950
Acceso en línea:https://doaj.org/article/4fcd90176046498484c3b51853f06eb8
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spelling oai:doaj.org-article:4fcd90176046498484c3b51853f06eb82021-11-25T16:23:53ZAnalysis on Drug-Resistance-Associated Mutations among Multidrug-Resistant <i>Mycobacterium tuberculosis</i> Isolates in China10.3390/antibiotics101113672079-6382https://doaj.org/article/4fcd90176046498484c3b51853f06eb82021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1367https://doaj.org/toc/2079-6382As the causative bacteria of tuberculosis, <i>Mycobacterium</i><i>tuberculosis (M. tb</i><i>)</i> is aggravated by the emergence of its multidrug-resistant isolates in China. Mutations of six of the most frequently reported resistant genes (<i>rpoB, katG, inhA, embB, gyrA,</i> and <i>rpsL</i>) were detected for rifampicin (RIF), isoniazid (INH), ethambutol (EMB), ofloxacin (OFX), and streptomycin (STR) in this study. The amino acid missense mutations (MMs) and their corresponding single nucleotide polymorphism mutations for all drug-resistant (DR) isolates are described in detail. All isolates were divided into non-extensively drug-resistant (Non-XDR) and preXDR/XDR groups. No statistical differences were detected among MMs and linked MMs (LMs) between the two groups, except for <i>rpsL</i> 88 (<i>p</i> = 0.037). In the preXDR/XDR group, the occurrence of MMs in <i>rpoB, katG,</i> and <i>inhA</i> developed phenotypic resistance and MMs of <i>rpoB</i> 531, <i>katG</i> 315, <i>rpsL</i> 43, and <i>rpsL</i> 88 could develop high levels of DR. It is necessary to carry out epidemiological investigations of DR gene mutations in the local region, and thus provide necessary data to support the design of new technologies for rapid detection of resistant <i>M. tb</i> and the optimization of detection targets.Hongbing JiaYuhui XuZhaogang SunMDPI AGarticle<i>Mycobacterium tuberculosis</i>drug-susceptibility testingmultidrug resistancemutationTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1367, p 1367 (2021)
institution DOAJ
collection DOAJ
language EN
topic <i>Mycobacterium tuberculosis</i>
drug-susceptibility testing
multidrug resistance
mutation
Therapeutics. Pharmacology
RM1-950
spellingShingle <i>Mycobacterium tuberculosis</i>
drug-susceptibility testing
multidrug resistance
mutation
Therapeutics. Pharmacology
RM1-950
Hongbing Jia
Yuhui Xu
Zhaogang Sun
Analysis on Drug-Resistance-Associated Mutations among Multidrug-Resistant <i>Mycobacterium tuberculosis</i> Isolates in China
description As the causative bacteria of tuberculosis, <i>Mycobacterium</i><i>tuberculosis (M. tb</i><i>)</i> is aggravated by the emergence of its multidrug-resistant isolates in China. Mutations of six of the most frequently reported resistant genes (<i>rpoB, katG, inhA, embB, gyrA,</i> and <i>rpsL</i>) were detected for rifampicin (RIF), isoniazid (INH), ethambutol (EMB), ofloxacin (OFX), and streptomycin (STR) in this study. The amino acid missense mutations (MMs) and their corresponding single nucleotide polymorphism mutations for all drug-resistant (DR) isolates are described in detail. All isolates were divided into non-extensively drug-resistant (Non-XDR) and preXDR/XDR groups. No statistical differences were detected among MMs and linked MMs (LMs) between the two groups, except for <i>rpsL</i> 88 (<i>p</i> = 0.037). In the preXDR/XDR group, the occurrence of MMs in <i>rpoB, katG,</i> and <i>inhA</i> developed phenotypic resistance and MMs of <i>rpoB</i> 531, <i>katG</i> 315, <i>rpsL</i> 43, and <i>rpsL</i> 88 could develop high levels of DR. It is necessary to carry out epidemiological investigations of DR gene mutations in the local region, and thus provide necessary data to support the design of new technologies for rapid detection of resistant <i>M. tb</i> and the optimization of detection targets.
format article
author Hongbing Jia
Yuhui Xu
Zhaogang Sun
author_facet Hongbing Jia
Yuhui Xu
Zhaogang Sun
author_sort Hongbing Jia
title Analysis on Drug-Resistance-Associated Mutations among Multidrug-Resistant <i>Mycobacterium tuberculosis</i> Isolates in China
title_short Analysis on Drug-Resistance-Associated Mutations among Multidrug-Resistant <i>Mycobacterium tuberculosis</i> Isolates in China
title_full Analysis on Drug-Resistance-Associated Mutations among Multidrug-Resistant <i>Mycobacterium tuberculosis</i> Isolates in China
title_fullStr Analysis on Drug-Resistance-Associated Mutations among Multidrug-Resistant <i>Mycobacterium tuberculosis</i> Isolates in China
title_full_unstemmed Analysis on Drug-Resistance-Associated Mutations among Multidrug-Resistant <i>Mycobacterium tuberculosis</i> Isolates in China
title_sort analysis on drug-resistance-associated mutations among multidrug-resistant <i>mycobacterium tuberculosis</i> isolates in china
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/4fcd90176046498484c3b51853f06eb8
work_keys_str_mv AT hongbingjia analysisondrugresistanceassociatedmutationsamongmultidrugresistantimycobacteriumtuberculosisiisolatesinchina
AT yuhuixu analysisondrugresistanceassociatedmutationsamongmultidrugresistantimycobacteriumtuberculosisiisolatesinchina
AT zhaogangsun analysisondrugresistanceassociatedmutationsamongmultidrugresistantimycobacteriumtuberculosisiisolatesinchina
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