Characterization of Circulating Low-Density Neutrophils Intrinsic Properties in Healthy and Asthmatic Horses

Abstract Low-density neutrophils (LDNs) are a subset of neutrophils first described in the bloodstream upon pathological conditions, and recently, in the blood of healthy humans. LDNs may have an enhanced pro-inflammatory (low-density granulocytes, LDGs) or an immunosuppressive (Granulocytic myeloid...

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Autores principales:	Nicolas Herteman, Amandine Vargas, Jean-Pierre Lavoie
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4fcebf78228b471c9019529fe008ec66
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spelling	oai:doaj.org-article:4fcebf78228b471c9019529fe008ec662021-12-02T16:06:26ZCharacterization of Circulating Low-Density Neutrophils Intrinsic Properties in Healthy and Asthmatic Horses10.1038/s41598-017-08089-52045-2322https://doaj.org/article/4fcebf78228b471c9019529fe008ec662017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08089-5https://doaj.org/toc/2045-2322Abstract Low-density neutrophils (LDNs) are a subset of neutrophils first described in the bloodstream upon pathological conditions, and recently, in the blood of healthy humans. LDNs may have an enhanced pro-inflammatory (low-density granulocytes, LDGs) or an immunosuppressive (Granulocytic myeloid-derived suppressor cells, G-MDSCs) profile. Whether these characteristics are specific to LDNs or related to disease states is unknown. Thus, we sought to investigate the properties of LDNs in both health and disease states, and to compare them to those of autologous normal-density neutrophils (NDNs). We studied 8 horses with severe equine asthma and 11 healthy animals. LDNs were smaller and contained more N-formylmethionine-leucyl-phenylalanine receptors than NDNs, but the myeloperoxidase content was similar in both cell populations. They also had an increased capacity to produce neutrophil extracellular traps, and were more sensitive to activation by phorbol-12-myristate-13-acetate. This profile is suggestive of LDGs. These characteristics were similar in both healthy and diseased animals, indicating that these are intrinsic properties of LDNs. Furthermore, these results suggest that LDNs represent a population of primed and predominantly mature cells. This study is the first to characterize LDNs in health, and to compare their properties with those of NDNs and of animals with a naturally occurring disease.Nicolas HertemanAmandine VargasJean-Pierre LavoieNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nicolas Herteman Amandine Vargas Jean-Pierre Lavoie Characterization of Circulating Low-Density Neutrophils Intrinsic Properties in Healthy and Asthmatic Horses
description	Abstract Low-density neutrophils (LDNs) are a subset of neutrophils first described in the bloodstream upon pathological conditions, and recently, in the blood of healthy humans. LDNs may have an enhanced pro-inflammatory (low-density granulocytes, LDGs) or an immunosuppressive (Granulocytic myeloid-derived suppressor cells, G-MDSCs) profile. Whether these characteristics are specific to LDNs or related to disease states is unknown. Thus, we sought to investigate the properties of LDNs in both health and disease states, and to compare them to those of autologous normal-density neutrophils (NDNs). We studied 8 horses with severe equine asthma and 11 healthy animals. LDNs were smaller and contained more N-formylmethionine-leucyl-phenylalanine receptors than NDNs, but the myeloperoxidase content was similar in both cell populations. They also had an increased capacity to produce neutrophil extracellular traps, and were more sensitive to activation by phorbol-12-myristate-13-acetate. This profile is suggestive of LDGs. These characteristics were similar in both healthy and diseased animals, indicating that these are intrinsic properties of LDNs. Furthermore, these results suggest that LDNs represent a population of primed and predominantly mature cells. This study is the first to characterize LDNs in health, and to compare their properties with those of NDNs and of animals with a naturally occurring disease.
format	article
author	Nicolas Herteman Amandine Vargas Jean-Pierre Lavoie
author_facet	Nicolas Herteman Amandine Vargas Jean-Pierre Lavoie
author_sort	Nicolas Herteman
title	Characterization of Circulating Low-Density Neutrophils Intrinsic Properties in Healthy and Asthmatic Horses
title_short	Characterization of Circulating Low-Density Neutrophils Intrinsic Properties in Healthy and Asthmatic Horses
title_full	Characterization of Circulating Low-Density Neutrophils Intrinsic Properties in Healthy and Asthmatic Horses
title_fullStr	Characterization of Circulating Low-Density Neutrophils Intrinsic Properties in Healthy and Asthmatic Horses
title_full_unstemmed	Characterization of Circulating Low-Density Neutrophils Intrinsic Properties in Healthy and Asthmatic Horses
title_sort	characterization of circulating low-density neutrophils intrinsic properties in healthy and asthmatic horses
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4fcebf78228b471c9019529fe008ec66
work_keys_str_mv	AT nicolasherteman characterizationofcirculatinglowdensityneutrophilsintrinsicpropertiesinhealthyandasthmatichorses AT amandinevargas characterizationofcirculatinglowdensityneutrophilsintrinsicpropertiesinhealthyandasthmatichorses AT jeanpierrelavoie characterizationofcirculatinglowdensityneutrophilsintrinsicpropertiesinhealthyandasthmatichorses
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Characterization of Circulating Low-Density Neutrophils Intrinsic Properties in Healthy and Asthmatic Horses

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