Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments
Abstract Meiotically arrested oocytes are characterized by the presence of the nuclear structure known as germinal-vesicle (GV), the breakdown of which (GVBD) is associated with resumption of meiosis. Fyn is a pivotal factor in resumption of the first meiotic division; its inhibition markedly decrea...
Guardado en:
Autores principales: | , , , , , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2017
|
Materias: | |
Acceso en línea: | https://doaj.org/article/4fd0b659895a46078f55198ea45040b7 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:4fd0b659895a46078f55198ea45040b7 |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:4fd0b659895a46078f55198ea45040b72021-12-02T12:32:26ZRegulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments10.1038/s41598-017-02071-x2045-2322https://doaj.org/article/4fd0b659895a46078f55198ea45040b72017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02071-xhttps://doaj.org/toc/2045-2322Abstract Meiotically arrested oocytes are characterized by the presence of the nuclear structure known as germinal-vesicle (GV), the breakdown of which (GVBD) is associated with resumption of meiosis. Fyn is a pivotal factor in resumption of the first meiotic division; its inhibition markedly decreases the fraction of oocytes undergoing GVBD. Here, we reveal that in mouse oocytes Fyn is post-transcriptionally regulated by miR-125a-3p. We demonstrate that in oocytes resuming meiosis miR-125a-3p and Fyn exhibit a reciprocal expression pattern; miR-125a-3p decreases alongside with an increase in Fyn expression. Microinjection of miR-125a-3p inhibits GVBD, an effect that is markedly reduced by Fyn over-expression, and impairs the organization of the actin rim surrounding the nucleus. Lower rate of GVBD is also observed in oocytes exposed to cytochalasin-D or blebbistatin, which interfere with actin polymerization and contractility of actin bundles, respectively. By down-regulating Fyn in HEK-293T cells, miR-125a-3p reduces the interaction between actin and A-type lamins, which constitute the nuclear-lamina. Our findings suggest a mechanism, by which a decrease in miR-125a-3p during oocyte maturation facilitates GVBD by allowing Fyn up-regulation and the resulting stabilization of the interaction between actin and A-type lamins.Hadas GrossmanEfrat Har-PazNatalie GindiMattan LeviIrit MillerNava NevoDalia GalianiNava DekelRuth ShalgiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Hadas Grossman Efrat Har-Paz Natalie Gindi Mattan Levi Irit Miller Nava Nevo Dalia Galiani Nava Dekel Ruth Shalgi Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments |
description |
Abstract Meiotically arrested oocytes are characterized by the presence of the nuclear structure known as germinal-vesicle (GV), the breakdown of which (GVBD) is associated with resumption of meiosis. Fyn is a pivotal factor in resumption of the first meiotic division; its inhibition markedly decreases the fraction of oocytes undergoing GVBD. Here, we reveal that in mouse oocytes Fyn is post-transcriptionally regulated by miR-125a-3p. We demonstrate that in oocytes resuming meiosis miR-125a-3p and Fyn exhibit a reciprocal expression pattern; miR-125a-3p decreases alongside with an increase in Fyn expression. Microinjection of miR-125a-3p inhibits GVBD, an effect that is markedly reduced by Fyn over-expression, and impairs the organization of the actin rim surrounding the nucleus. Lower rate of GVBD is also observed in oocytes exposed to cytochalasin-D or blebbistatin, which interfere with actin polymerization and contractility of actin bundles, respectively. By down-regulating Fyn in HEK-293T cells, miR-125a-3p reduces the interaction between actin and A-type lamins, which constitute the nuclear-lamina. Our findings suggest a mechanism, by which a decrease in miR-125a-3p during oocyte maturation facilitates GVBD by allowing Fyn up-regulation and the resulting stabilization of the interaction between actin and A-type lamins. |
format |
article |
author |
Hadas Grossman Efrat Har-Paz Natalie Gindi Mattan Levi Irit Miller Nava Nevo Dalia Galiani Nava Dekel Ruth Shalgi |
author_facet |
Hadas Grossman Efrat Har-Paz Natalie Gindi Mattan Levi Irit Miller Nava Nevo Dalia Galiani Nava Dekel Ruth Shalgi |
author_sort |
Hadas Grossman |
title |
Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments |
title_short |
Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments |
title_full |
Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments |
title_fullStr |
Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments |
title_full_unstemmed |
Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments |
title_sort |
regulation of gvbd in mouse oocytes by mir-125a-3p and fyn kinase through modulation of actin filaments |
publisher |
Nature Portfolio |
publishDate |
2017 |
url |
https://doaj.org/article/4fd0b659895a46078f55198ea45040b7 |
work_keys_str_mv |
AT hadasgrossman regulationofgvbdinmouseoocytesbymir125a3pandfynkinasethroughmodulationofactinfilaments AT efratharpaz regulationofgvbdinmouseoocytesbymir125a3pandfynkinasethroughmodulationofactinfilaments AT nataliegindi regulationofgvbdinmouseoocytesbymir125a3pandfynkinasethroughmodulationofactinfilaments AT mattanlevi regulationofgvbdinmouseoocytesbymir125a3pandfynkinasethroughmodulationofactinfilaments AT iritmiller regulationofgvbdinmouseoocytesbymir125a3pandfynkinasethroughmodulationofactinfilaments AT navanevo regulationofgvbdinmouseoocytesbymir125a3pandfynkinasethroughmodulationofactinfilaments AT daliagaliani regulationofgvbdinmouseoocytesbymir125a3pandfynkinasethroughmodulationofactinfilaments AT navadekel regulationofgvbdinmouseoocytesbymir125a3pandfynkinasethroughmodulationofactinfilaments AT ruthshalgi regulationofgvbdinmouseoocytesbymir125a3pandfynkinasethroughmodulationofactinfilaments |
_version_ |
1718394092960874496 |