Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments

Abstract Meiotically arrested oocytes are characterized by the presence of the nuclear structure known as germinal-vesicle (GV), the breakdown of which (GVBD) is associated with resumption of meiosis. Fyn is a pivotal factor in resumption of the first meiotic division; its inhibition markedly decrea...

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Autores principales: Hadas Grossman, Efrat Har-Paz, Natalie Gindi, Mattan Levi, Irit Miller, Nava Nevo, Dalia Galiani, Nava Dekel, Ruth Shalgi
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Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/4fd0b659895a46078f55198ea45040b7
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spelling oai:doaj.org-article:4fd0b659895a46078f55198ea45040b72021-12-02T12:32:26ZRegulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments10.1038/s41598-017-02071-x2045-2322https://doaj.org/article/4fd0b659895a46078f55198ea45040b72017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02071-xhttps://doaj.org/toc/2045-2322Abstract Meiotically arrested oocytes are characterized by the presence of the nuclear structure known as germinal-vesicle (GV), the breakdown of which (GVBD) is associated with resumption of meiosis. Fyn is a pivotal factor in resumption of the first meiotic division; its inhibition markedly decreases the fraction of oocytes undergoing GVBD. Here, we reveal that in mouse oocytes Fyn is post-transcriptionally regulated by miR-125a-3p. We demonstrate that in oocytes resuming meiosis miR-125a-3p and Fyn exhibit a reciprocal expression pattern; miR-125a-3p decreases alongside with an increase in Fyn expression. Microinjection of miR-125a-3p inhibits GVBD, an effect that is markedly reduced by Fyn over-expression, and impairs the organization of the actin rim surrounding the nucleus. Lower rate of GVBD is also observed in oocytes exposed to cytochalasin-D or blebbistatin, which interfere with actin polymerization and contractility of actin bundles, respectively. By down-regulating Fyn in HEK-293T cells, miR-125a-3p reduces the interaction between actin and A-type lamins, which constitute the nuclear-lamina. Our findings suggest a mechanism, by which a decrease in miR-125a-3p during oocyte maturation facilitates GVBD by allowing Fyn up-regulation and the resulting stabilization of the interaction between actin and A-type lamins.Hadas GrossmanEfrat Har-PazNatalie GindiMattan LeviIrit MillerNava NevoDalia GalianiNava DekelRuth ShalgiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Hadas Grossman
Efrat Har-Paz
Natalie Gindi
Mattan Levi
Irit Miller
Nava Nevo
Dalia Galiani
Nava Dekel
Ruth Shalgi
Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments
description Abstract Meiotically arrested oocytes are characterized by the presence of the nuclear structure known as germinal-vesicle (GV), the breakdown of which (GVBD) is associated with resumption of meiosis. Fyn is a pivotal factor in resumption of the first meiotic division; its inhibition markedly decreases the fraction of oocytes undergoing GVBD. Here, we reveal that in mouse oocytes Fyn is post-transcriptionally regulated by miR-125a-3p. We demonstrate that in oocytes resuming meiosis miR-125a-3p and Fyn exhibit a reciprocal expression pattern; miR-125a-3p decreases alongside with an increase in Fyn expression. Microinjection of miR-125a-3p inhibits GVBD, an effect that is markedly reduced by Fyn over-expression, and impairs the organization of the actin rim surrounding the nucleus. Lower rate of GVBD is also observed in oocytes exposed to cytochalasin-D or blebbistatin, which interfere with actin polymerization and contractility of actin bundles, respectively. By down-regulating Fyn in HEK-293T cells, miR-125a-3p reduces the interaction between actin and A-type lamins, which constitute the nuclear-lamina. Our findings suggest a mechanism, by which a decrease in miR-125a-3p during oocyte maturation facilitates GVBD by allowing Fyn up-regulation and the resulting stabilization of the interaction between actin and A-type lamins.
format article
author Hadas Grossman
Efrat Har-Paz
Natalie Gindi
Mattan Levi
Irit Miller
Nava Nevo
Dalia Galiani
Nava Dekel
Ruth Shalgi
author_facet Hadas Grossman
Efrat Har-Paz
Natalie Gindi
Mattan Levi
Irit Miller
Nava Nevo
Dalia Galiani
Nava Dekel
Ruth Shalgi
author_sort Hadas Grossman
title Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments
title_short Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments
title_full Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments
title_fullStr Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments
title_full_unstemmed Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments
title_sort regulation of gvbd in mouse oocytes by mir-125a-3p and fyn kinase through modulation of actin filaments
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/4fd0b659895a46078f55198ea45040b7
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