Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments

Abstract Meiotically arrested oocytes are characterized by the presence of the nuclear structure known as germinal-vesicle (GV), the breakdown of which (GVBD) is associated with resumption of meiosis. Fyn is a pivotal factor in resumption of the first meiotic division; its inhibition markedly decrea...

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Autores principales:	Hadas Grossman, Efrat Har-Paz, Natalie Gindi, Mattan Levi, Irit Miller, Nava Nevo, Dalia Galiani, Nava Dekel, Ruth Shalgi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4fd0b659895a46078f55198ea45040b7
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spelling	oai:doaj.org-article:4fd0b659895a46078f55198ea45040b72021-12-02T12:32:26ZRegulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments10.1038/s41598-017-02071-x2045-2322https://doaj.org/article/4fd0b659895a46078f55198ea45040b72017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02071-xhttps://doaj.org/toc/2045-2322Abstract Meiotically arrested oocytes are characterized by the presence of the nuclear structure known as germinal-vesicle (GV), the breakdown of which (GVBD) is associated with resumption of meiosis. Fyn is a pivotal factor in resumption of the first meiotic division; its inhibition markedly decreases the fraction of oocytes undergoing GVBD. Here, we reveal that in mouse oocytes Fyn is post-transcriptionally regulated by miR-125a-3p. We demonstrate that in oocytes resuming meiosis miR-125a-3p and Fyn exhibit a reciprocal expression pattern; miR-125a-3p decreases alongside with an increase in Fyn expression. Microinjection of miR-125a-3p inhibits GVBD, an effect that is markedly reduced by Fyn over-expression, and impairs the organization of the actin rim surrounding the nucleus. Lower rate of GVBD is also observed in oocytes exposed to cytochalasin-D or blebbistatin, which interfere with actin polymerization and contractility of actin bundles, respectively. By down-regulating Fyn in HEK-293T cells, miR-125a-3p reduces the interaction between actin and A-type lamins, which constitute the nuclear-lamina. Our findings suggest a mechanism, by which a decrease in miR-125a-3p during oocyte maturation facilitates GVBD by allowing Fyn up-regulation and the resulting stabilization of the interaction between actin and A-type lamins.Hadas GrossmanEfrat Har-PazNatalie GindiMattan LeviIrit MillerNava NevoDalia GalianiNava DekelRuth ShalgiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hadas Grossman Efrat Har-Paz Natalie Gindi Mattan Levi Irit Miller Nava Nevo Dalia Galiani Nava Dekel Ruth Shalgi Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments
description	Abstract Meiotically arrested oocytes are characterized by the presence of the nuclear structure known as germinal-vesicle (GV), the breakdown of which (GVBD) is associated with resumption of meiosis. Fyn is a pivotal factor in resumption of the first meiotic division; its inhibition markedly decreases the fraction of oocytes undergoing GVBD. Here, we reveal that in mouse oocytes Fyn is post-transcriptionally regulated by miR-125a-3p. We demonstrate that in oocytes resuming meiosis miR-125a-3p and Fyn exhibit a reciprocal expression pattern; miR-125a-3p decreases alongside with an increase in Fyn expression. Microinjection of miR-125a-3p inhibits GVBD, an effect that is markedly reduced by Fyn over-expression, and impairs the organization of the actin rim surrounding the nucleus. Lower rate of GVBD is also observed in oocytes exposed to cytochalasin-D or blebbistatin, which interfere with actin polymerization and contractility of actin bundles, respectively. By down-regulating Fyn in HEK-293T cells, miR-125a-3p reduces the interaction between actin and A-type lamins, which constitute the nuclear-lamina. Our findings suggest a mechanism, by which a decrease in miR-125a-3p during oocyte maturation facilitates GVBD by allowing Fyn up-regulation and the resulting stabilization of the interaction between actin and A-type lamins.
format	article
author	Hadas Grossman Efrat Har-Paz Natalie Gindi Mattan Levi Irit Miller Nava Nevo Dalia Galiani Nava Dekel Ruth Shalgi
author_facet	Hadas Grossman Efrat Har-Paz Natalie Gindi Mattan Levi Irit Miller Nava Nevo Dalia Galiani Nava Dekel Ruth Shalgi
author_sort	Hadas Grossman
title	Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments
title_short	Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments
title_full	Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments
title_fullStr	Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments
title_full_unstemmed	Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments
title_sort	regulation of gvbd in mouse oocytes by mir-125a-3p and fyn kinase through modulation of actin filaments
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4fd0b659895a46078f55198ea45040b7
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Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments

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