Transcriptomic landscape of blood platelets in healthy donors

Abstract Blood platelet RNA-sequencing is increasingly used among the scientific community. Aberrant platelet transcriptome is common in cancer or cardiovascular disease, but reference data on platelet RNA content in healthy individuals are scarce and merit complex investigation. We sought to explor...

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Autores principales:	Anna Supernat, Marta Popęda, Krzysztof Pastuszak, Myron G. Best, Peter Grešner, Sjors In ’t Veld, Bartłomiej Siek, Natalia Bednarz-Knoll, Matthew T. Rondina, Tomasz Stokowy, Thomas Wurdinger, Jacek Jassem, Anna J. Żaczek
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4fd18847df6c4fb09343afbf7e38a8f5
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spelling	oai:doaj.org-article:4fd18847df6c4fb09343afbf7e38a8f52021-12-02T17:06:09ZTranscriptomic landscape of blood platelets in healthy donors10.1038/s41598-021-94003-z2045-2322https://doaj.org/article/4fd18847df6c4fb09343afbf7e38a8f52021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94003-zhttps://doaj.org/toc/2045-2322Abstract Blood platelet RNA-sequencing is increasingly used among the scientific community. Aberrant platelet transcriptome is common in cancer or cardiovascular disease, but reference data on platelet RNA content in healthy individuals are scarce and merit complex investigation. We sought to explore the dynamics of platelet transcriptome. Datasets from 204 healthy donors were used for the analysis of splice variants, particularly with regard to age, sex, blood storage time, unit of collection or library size. Genes B2M, PPBP, TMSB4X, ACTB, FTL, CLU, PF4, F13A1, GNAS, SPARC, PTMA, TAGLN2, OAZ1 and OST4 demonstrated the highest expression in the analysed cohort, remaining substantial transcription consistency. CSF3R gene was found upregulated in males (fold change 2.10, FDR q < 0.05). Cohort dichotomisation according to the median age, showed upregulated KSR1 in the older donors (fold change 2.11, FDR q < 0.05). Unsupervised hierarchical clustering revealed two clusters which were irrespective of age, sex, storage time, collecting unit or library size. However, when donors are analysed globally (as vectors), sex, storage time, library size, the unit of blood collection as well as age impose a certain degree of between- and/or within-group variability. Healthy donor platelet transcriptome retains general consistency, with very few splice variants deviating from the landscape. Although multidimensional analysis reveals statistically significant variability between and within the analysed groups, biologically, these changes are minor and irrelevant while considering disease classification. Our work provides a reference for studies working both on healthy platelets and pathological conditions affecting platelet transcriptome.Anna SupernatMarta PopędaKrzysztof PastuszakMyron G. BestPeter GrešnerSjors In ’t VeldBartłomiej SiekNatalia Bednarz-KnollMatthew T. RondinaTomasz StokowyThomas WurdingerJacek JassemAnna J. ŻaczekNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna Supernat Marta Popęda Krzysztof Pastuszak Myron G. Best Peter Grešner Sjors In ’t Veld Bartłomiej Siek Natalia Bednarz-Knoll Matthew T. Rondina Tomasz Stokowy Thomas Wurdinger Jacek Jassem Anna J. Żaczek Transcriptomic landscape of blood platelets in healthy donors
description	Abstract Blood platelet RNA-sequencing is increasingly used among the scientific community. Aberrant platelet transcriptome is common in cancer or cardiovascular disease, but reference data on platelet RNA content in healthy individuals are scarce and merit complex investigation. We sought to explore the dynamics of platelet transcriptome. Datasets from 204 healthy donors were used for the analysis of splice variants, particularly with regard to age, sex, blood storage time, unit of collection or library size. Genes B2M, PPBP, TMSB4X, ACTB, FTL, CLU, PF4, F13A1, GNAS, SPARC, PTMA, TAGLN2, OAZ1 and OST4 demonstrated the highest expression in the analysed cohort, remaining substantial transcription consistency. CSF3R gene was found upregulated in males (fold change 2.10, FDR q < 0.05). Cohort dichotomisation according to the median age, showed upregulated KSR1 in the older donors (fold change 2.11, FDR q < 0.05). Unsupervised hierarchical clustering revealed two clusters which were irrespective of age, sex, storage time, collecting unit or library size. However, when donors are analysed globally (as vectors), sex, storage time, library size, the unit of blood collection as well as age impose a certain degree of between- and/or within-group variability. Healthy donor platelet transcriptome retains general consistency, with very few splice variants deviating from the landscape. Although multidimensional analysis reveals statistically significant variability between and within the analysed groups, biologically, these changes are minor and irrelevant while considering disease classification. Our work provides a reference for studies working both on healthy platelets and pathological conditions affecting platelet transcriptome.
format	article
author	Anna Supernat Marta Popęda Krzysztof Pastuszak Myron G. Best Peter Grešner Sjors In ’t Veld Bartłomiej Siek Natalia Bednarz-Knoll Matthew T. Rondina Tomasz Stokowy Thomas Wurdinger Jacek Jassem Anna J. Żaczek
author_facet	Anna Supernat Marta Popęda Krzysztof Pastuszak Myron G. Best Peter Grešner Sjors In ’t Veld Bartłomiej Siek Natalia Bednarz-Knoll Matthew T. Rondina Tomasz Stokowy Thomas Wurdinger Jacek Jassem Anna J. Żaczek
author_sort	Anna Supernat
title	Transcriptomic landscape of blood platelets in healthy donors
title_short	Transcriptomic landscape of blood platelets in healthy donors
title_full	Transcriptomic landscape of blood platelets in healthy donors
title_fullStr	Transcriptomic landscape of blood platelets in healthy donors
title_full_unstemmed	Transcriptomic landscape of blood platelets in healthy donors
title_sort	transcriptomic landscape of blood platelets in healthy donors
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4fd18847df6c4fb09343afbf7e38a8f5
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_version_	1718381729380564992

Transcriptomic landscape of blood platelets in healthy donors

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