A high-throughput protocol for monitoring starvation-induced autophagy in real time in mouse embryonic fibroblasts

Summary: Autophagy measurement has been challenging due to the transient nature of autophagy vesicles, in which degradation of cargo occurs. Here, we present a protocol to monitor starvation-induced autophagy using a live high-throughput microscopy system in a fast and automated manner without the n...

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Autores principales: Ada Nowosad, Arnaud Besson
Formato: article
Lenguaje:EN
Publicado: Elsevier 2021
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Acceso en línea:https://doaj.org/article/4fd57986613f436d96f10c6836e8d563
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