A high-throughput protocol for monitoring starvation-induced autophagy in real time in mouse embryonic fibroblasts
Summary: Autophagy measurement has been challenging due to the transient nature of autophagy vesicles, in which degradation of cargo occurs. Here, we present a protocol to monitor starvation-induced autophagy using a live high-throughput microscopy system in a fast and automated manner without the n...
Guardado en:
Autores principales: | Ada Nowosad, Arnaud Besson |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://doaj.org/article/4fd57986613f436d96f10c6836e8d563 |
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