Visualizing Micro-anatomical Structures of the Posterior Cornea with Micro-optical Coherence Tomography

Abstract Diagnosis of corneal disease and challenges in corneal transplantation require comprehensive understanding of corneal anatomy, particularly that of the posterior cornea. Micro-optical coherence tomography (µOCT) is a potentially suitable tool to meet this need, owing to its ultrahigh isotro...

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Autores principales:	Si Chen, Xinyu Liu, Nanshuo Wang, Xianghong Wang, Qiaozhou Xiong, En Bo, Xiaojun Yu, Shufen Chen, Linbo Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4fd6e2055aec4a3f8b2deccbb81965ba
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spelling	oai:doaj.org-article:4fd6e2055aec4a3f8b2deccbb81965ba2021-12-02T15:05:55ZVisualizing Micro-anatomical Structures of the Posterior Cornea with Micro-optical Coherence Tomography10.1038/s41598-017-11380-02045-2322https://doaj.org/article/4fd6e2055aec4a3f8b2deccbb81965ba2017-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-11380-0https://doaj.org/toc/2045-2322Abstract Diagnosis of corneal disease and challenges in corneal transplantation require comprehensive understanding of corneal anatomy, particularly that of the posterior cornea. Micro-optical coherence tomography (µOCT) is a potentially suitable tool to meet this need, owing to its ultrahigh isotropic spatial resolution, high image acquisition rate and depth priority scanning mode. In this study, we explored the ability of µOCT to visualize micro-anatomical structures of the posterior cornea ex vivo and in vivo using small and large animals. µOCT clearly delineated cornea layers and revealed micro-anatomical structures, including not only polygonal endothelial cells, stellate keratocytes, collagen fibres and corneal nerve fibres but also new structures such as the dome-shaped basolateral side of endothelial cells and lattice structures at the interface between endothelium and Descemet’s membrane. Based on these observations, a short post-harvest longitudinal study was conducted on rat cornea to test the feasibility of using µOCT to monitor the quality of endothelial cells. This study successfully reveals a series of morphological features and pathological changes in the posterior cornea at the cellular level in situ and in real time with µOCT. These findings enrich knowledge of corneal anatomy and suggest that µOCT may be a promising imaging tool in corneal transplantation.Si ChenXinyu LiuNanshuo WangXianghong WangQiaozhou XiongEn BoXiaojun YuShufen ChenLinbo LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Si Chen Xinyu Liu Nanshuo Wang Xianghong Wang Qiaozhou Xiong En Bo Xiaojun Yu Shufen Chen Linbo Liu Visualizing Micro-anatomical Structures of the Posterior Cornea with Micro-optical Coherence Tomography
description	Abstract Diagnosis of corneal disease and challenges in corneal transplantation require comprehensive understanding of corneal anatomy, particularly that of the posterior cornea. Micro-optical coherence tomography (µOCT) is a potentially suitable tool to meet this need, owing to its ultrahigh isotropic spatial resolution, high image acquisition rate and depth priority scanning mode. In this study, we explored the ability of µOCT to visualize micro-anatomical structures of the posterior cornea ex vivo and in vivo using small and large animals. µOCT clearly delineated cornea layers and revealed micro-anatomical structures, including not only polygonal endothelial cells, stellate keratocytes, collagen fibres and corneal nerve fibres but also new structures such as the dome-shaped basolateral side of endothelial cells and lattice structures at the interface between endothelium and Descemet’s membrane. Based on these observations, a short post-harvest longitudinal study was conducted on rat cornea to test the feasibility of using µOCT to monitor the quality of endothelial cells. This study successfully reveals a series of morphological features and pathological changes in the posterior cornea at the cellular level in situ and in real time with µOCT. These findings enrich knowledge of corneal anatomy and suggest that µOCT may be a promising imaging tool in corneal transplantation.
format	article
author	Si Chen Xinyu Liu Nanshuo Wang Xianghong Wang Qiaozhou Xiong En Bo Xiaojun Yu Shufen Chen Linbo Liu
author_facet	Si Chen Xinyu Liu Nanshuo Wang Xianghong Wang Qiaozhou Xiong En Bo Xiaojun Yu Shufen Chen Linbo Liu
author_sort	Si Chen
title	Visualizing Micro-anatomical Structures of the Posterior Cornea with Micro-optical Coherence Tomography
title_short	Visualizing Micro-anatomical Structures of the Posterior Cornea with Micro-optical Coherence Tomography
title_full	Visualizing Micro-anatomical Structures of the Posterior Cornea with Micro-optical Coherence Tomography
title_fullStr	Visualizing Micro-anatomical Structures of the Posterior Cornea with Micro-optical Coherence Tomography
title_full_unstemmed	Visualizing Micro-anatomical Structures of the Posterior Cornea with Micro-optical Coherence Tomography
title_sort	visualizing micro-anatomical structures of the posterior cornea with micro-optical coherence tomography
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4fd6e2055aec4a3f8b2deccbb81965ba
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Visualizing Micro-anatomical Structures of the Posterior Cornea with Micro-optical Coherence Tomography

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