Effects of spaceflight aboard the International Space Station on mouse estrous cycle and ovarian gene expression

Abstract Ovarian steroids dramatically impact normal homeostatic and metabolic processes of most tissues within the body, including muscle, bone, neural, immune, cardiovascular, and reproductive systems. Determining the effects of spaceflight on the ovary and estrous cycle is, therefore, critical to...

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Autores principales:	Xiaoman Hong, Anamika Ratri, Sungshin Y. Choi, Joseph S. Tash, April E. Ronca, Joshua S. Alwood, Lane K. Christenson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biotechnology TP248.13-248.65 Physiology QP1-981
Acceso en línea:	https://doaj.org/article/4fdd39a8dc864f128470ff20d63eda80
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spelling	oai:doaj.org-article:4fdd39a8dc864f128470ff20d63eda802021-12-02T13:15:50ZEffects of spaceflight aboard the International Space Station on mouse estrous cycle and ovarian gene expression10.1038/s41526-021-00139-72373-8065https://doaj.org/article/4fdd39a8dc864f128470ff20d63eda802021-03-01T00:00:00Zhttps://doi.org/10.1038/s41526-021-00139-7https://doaj.org/toc/2373-8065Abstract Ovarian steroids dramatically impact normal homeostatic and metabolic processes of most tissues within the body, including muscle, bone, neural, immune, cardiovascular, and reproductive systems. Determining the effects of spaceflight on the ovary and estrous cycle is, therefore, critical to our understanding of all spaceflight experiments using female mice. Adult female mice (n = 10) were exposed to and sacrificed on-orbit after 37 days of spaceflight in microgravity. Contemporary control (preflight baseline, vivarium, and habitat; n = 10/group) groups were maintained at the Kennedy Space Center, prior to sacrifice and similar tissue collection at the NASA Ames Research Center. Ovarian tissues were collected and processed for RNA and steroid analyses at initial carcass thaw. Vaginal wall tissue collected from twice frozen/thawed carcasses was fixed for estrous cycle stage determinations. The proportion of animals in each phase of the estrous cycle (i.e., proestrus, estrus, metestrus, and diestrus) did not appreciably differ between baseline, vivarium, and flight mice, while habitat control mice exhibited greater numbers in diestrus. Ovarian tissue steroid concentrations indicated no differences in estradiol across groups, while progesterone levels were lower (p < 0.05) in habitat and flight compared to baseline females. Genes involved in ovarian steroidogenic function were not differentially expressed across groups. As ovarian estrogen can dramatically impact multiple non-reproductive tissues, these data support vaginal wall estrous cycle classification of all female mice flown in space. Additionally, since females exposed to long-term spaceflight were observed at different estrous cycle stages, this indicates females are likely undergoing ovarian cyclicity and may yet be fertile.Xiaoman HongAnamika RatriSungshin Y. ChoiJoseph S. TashApril E. RoncaJoshua S. AlwoodLane K. ChristensonNature PortfolioarticleBiotechnologyTP248.13-248.65PhysiologyQP1-981ENnpj Microgravity, Vol 7, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65 Physiology QP1-981
spellingShingle	Biotechnology TP248.13-248.65 Physiology QP1-981 Xiaoman Hong Anamika Ratri Sungshin Y. Choi Joseph S. Tash April E. Ronca Joshua S. Alwood Lane K. Christenson Effects of spaceflight aboard the International Space Station on mouse estrous cycle and ovarian gene expression
description	Abstract Ovarian steroids dramatically impact normal homeostatic and metabolic processes of most tissues within the body, including muscle, bone, neural, immune, cardiovascular, and reproductive systems. Determining the effects of spaceflight on the ovary and estrous cycle is, therefore, critical to our understanding of all spaceflight experiments using female mice. Adult female mice (n = 10) were exposed to and sacrificed on-orbit after 37 days of spaceflight in microgravity. Contemporary control (preflight baseline, vivarium, and habitat; n = 10/group) groups were maintained at the Kennedy Space Center, prior to sacrifice and similar tissue collection at the NASA Ames Research Center. Ovarian tissues were collected and processed for RNA and steroid analyses at initial carcass thaw. Vaginal wall tissue collected from twice frozen/thawed carcasses was fixed for estrous cycle stage determinations. The proportion of animals in each phase of the estrous cycle (i.e., proestrus, estrus, metestrus, and diestrus) did not appreciably differ between baseline, vivarium, and flight mice, while habitat control mice exhibited greater numbers in diestrus. Ovarian tissue steroid concentrations indicated no differences in estradiol across groups, while progesterone levels were lower (p < 0.05) in habitat and flight compared to baseline females. Genes involved in ovarian steroidogenic function were not differentially expressed across groups. As ovarian estrogen can dramatically impact multiple non-reproductive tissues, these data support vaginal wall estrous cycle classification of all female mice flown in space. Additionally, since females exposed to long-term spaceflight were observed at different estrous cycle stages, this indicates females are likely undergoing ovarian cyclicity and may yet be fertile.
format	article
author	Xiaoman Hong Anamika Ratri Sungshin Y. Choi Joseph S. Tash April E. Ronca Joshua S. Alwood Lane K. Christenson
author_facet	Xiaoman Hong Anamika Ratri Sungshin Y. Choi Joseph S. Tash April E. Ronca Joshua S. Alwood Lane K. Christenson
author_sort	Xiaoman Hong
title	Effects of spaceflight aboard the International Space Station on mouse estrous cycle and ovarian gene expression
title_short	Effects of spaceflight aboard the International Space Station on mouse estrous cycle and ovarian gene expression
title_full	Effects of spaceflight aboard the International Space Station on mouse estrous cycle and ovarian gene expression
title_fullStr	Effects of spaceflight aboard the International Space Station on mouse estrous cycle and ovarian gene expression
title_full_unstemmed	Effects of spaceflight aboard the International Space Station on mouse estrous cycle and ovarian gene expression
title_sort	effects of spaceflight aboard the international space station on mouse estrous cycle and ovarian gene expression
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4fdd39a8dc864f128470ff20d63eda80
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Effects of spaceflight aboard the International Space Station on mouse estrous cycle and ovarian gene expression

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