Application of phage display technology for the production of antibodies against Streptococcus suis serotype 2.

Streptococcus suis (S. suis) serotype 2 infection is a problem in the swine industry and responsible for most cases of human infection worldwide. Since current multiplex PCR cannot differentiate between serotypes 2 and 1/2, then serotype-specific antibodies (Abs) are required for serotype identifica...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Pattarawadee Sulong, Natsinee Anudit, Suphachai Nuanualsuwan, Segura Mariela, Kannika Khantasup
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4fdd4cfd9b0c489dbccf7184a689f886
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4fdd4cfd9b0c489dbccf7184a689f886
record_format	dspace
spelling	oai:doaj.org-article:4fdd4cfd9b0c489dbccf7184a689f8862021-12-02T20:13:30ZApplication of phage display technology for the production of antibodies against Streptococcus suis serotype 2.1932-620310.1371/journal.pone.0258931https://doaj.org/article/4fdd4cfd9b0c489dbccf7184a689f8862021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258931https://doaj.org/toc/1932-6203Streptococcus suis (S. suis) serotype 2 infection is a problem in the swine industry and responsible for most cases of human infection worldwide. Since current multiplex PCR cannot differentiate between serotypes 2 and 1/2, then serotype-specific antibodies (Abs) are required for serotype identification to confirm infection by serotype 2. This study aimed to generate Abs specific to S. suis serotype 2 by phage display from a human heavy chain variable domain (VH) antibody library. For biopanning, whole cells of S. suis serotype 2 were used as the target antigen. With increasing selection stringency, we could select the VH Abs that specifically bound to a S. suis serotype 2 surface antigen, which was identified as the capsular polysaccharide (CPS). From ELISA analysis, the specific phage clone 47B3 VH with the highest binding activity to S. suis serotype 2 was selected and shown to have no cross-reactivity with S. suis serotypes 1/2, 1, and 14 that shared a common epitope with serotype 2 and occasionally cause infections in human. Moreover, no cross-reactivity with other bacteria that can be found in septic blood specimens was also observed. Then, 47B3 VH was successfully expressed as soluble 47B3 VH in E. coli TG1. The soluble 47B3 VH crude extract was further tested for its binding ability in a dose-dependent ELISA assay. The results indicated that the activity of phage clone 47B3 was still retained even when the Ab occurred in the soluble form. A quellung reaction demonstrated that the soluble 47B3 VH Ab could show bioactivity by differentiation between S. suis serotypes 2 and 1/2. Thus, it will be beneficial to use this VH Ab in the diagnosis of disease or discrimination of S. suis serotypes Furthermore, the results described here could motivate the use of phage display VH platform to produce serotyping antibodies.Pattarawadee SulongNatsinee AnuditSuphachai NuanualsuwanSegura MarielaKannika KhantasupPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0258931 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pattarawadee Sulong Natsinee Anudit Suphachai Nuanualsuwan Segura Mariela Kannika Khantasup Application of phage display technology for the production of antibodies against Streptococcus suis serotype 2.
description	Streptococcus suis (S. suis) serotype 2 infection is a problem in the swine industry and responsible for most cases of human infection worldwide. Since current multiplex PCR cannot differentiate between serotypes 2 and 1/2, then serotype-specific antibodies (Abs) are required for serotype identification to confirm infection by serotype 2. This study aimed to generate Abs specific to S. suis serotype 2 by phage display from a human heavy chain variable domain (VH) antibody library. For biopanning, whole cells of S. suis serotype 2 were used as the target antigen. With increasing selection stringency, we could select the VH Abs that specifically bound to a S. suis serotype 2 surface antigen, which was identified as the capsular polysaccharide (CPS). From ELISA analysis, the specific phage clone 47B3 VH with the highest binding activity to S. suis serotype 2 was selected and shown to have no cross-reactivity with S. suis serotypes 1/2, 1, and 14 that shared a common epitope with serotype 2 and occasionally cause infections in human. Moreover, no cross-reactivity with other bacteria that can be found in septic blood specimens was also observed. Then, 47B3 VH was successfully expressed as soluble 47B3 VH in E. coli TG1. The soluble 47B3 VH crude extract was further tested for its binding ability in a dose-dependent ELISA assay. The results indicated that the activity of phage clone 47B3 was still retained even when the Ab occurred in the soluble form. A quellung reaction demonstrated that the soluble 47B3 VH Ab could show bioactivity by differentiation between S. suis serotypes 2 and 1/2. Thus, it will be beneficial to use this VH Ab in the diagnosis of disease or discrimination of S. suis serotypes Furthermore, the results described here could motivate the use of phage display VH platform to produce serotyping antibodies.
format	article
author	Pattarawadee Sulong Natsinee Anudit Suphachai Nuanualsuwan Segura Mariela Kannika Khantasup
author_facet	Pattarawadee Sulong Natsinee Anudit Suphachai Nuanualsuwan Segura Mariela Kannika Khantasup
author_sort	Pattarawadee Sulong
title	Application of phage display technology for the production of antibodies against Streptococcus suis serotype 2.
title_short	Application of phage display technology for the production of antibodies against Streptococcus suis serotype 2.
title_full	Application of phage display technology for the production of antibodies against Streptococcus suis serotype 2.
title_fullStr	Application of phage display technology for the production of antibodies against Streptococcus suis serotype 2.
title_full_unstemmed	Application of phage display technology for the production of antibodies against Streptococcus suis serotype 2.
title_sort	application of phage display technology for the production of antibodies against streptococcus suis serotype 2.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/4fdd4cfd9b0c489dbccf7184a689f886
work_keys_str_mv	AT pattarawadeesulong applicationofphagedisplaytechnologyfortheproductionofantibodiesagainststreptococcussuisserotype2 AT natsineeanudit applicationofphagedisplaytechnologyfortheproductionofantibodiesagainststreptococcussuisserotype2 AT suphachainuanualsuwan applicationofphagedisplaytechnologyfortheproductionofantibodiesagainststreptococcussuisserotype2 AT seguramariela applicationofphagedisplaytechnologyfortheproductionofantibodiesagainststreptococcussuisserotype2 AT kannikakhantasup applicationofphagedisplaytechnologyfortheproductionofantibodiesagainststreptococcussuisserotype2
_version_	1718374763723751424

Application of phage display technology for the production of antibodies against Streptococcus suis serotype 2.

Ejemplares similares