Engineering designer beta cells with a CRISPR-Cas9 conjugation platform

Cas9 fusions partners are often limited to natural polypeptide chains at the Cas9 termni. Here the authors present a platform for site-specific and multiple-site conjugation to both termini and internal sites of Cas9, and they apply this platform to efficiently engineer insulin-producing β cells.

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Autores principales:	Donghyun Lim, Vedagopuram Sreekanth, Kurt J. Cox, Benjamin K. Law, Bridget K. Wagner, Jeffrey M. Karp, Amit Choudhary
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4fe0e563f9774b8ea8aa70b031449f72
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spelling	oai:doaj.org-article:4fe0e563f9774b8ea8aa70b031449f722021-12-02T16:27:46ZEngineering designer beta cells with a CRISPR-Cas9 conjugation platform10.1038/s41467-020-17725-02041-1723https://doaj.org/article/4fe0e563f9774b8ea8aa70b031449f722020-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17725-0https://doaj.org/toc/2041-1723Cas9 fusions partners are often limited to natural polypeptide chains at the Cas9 termni. Here the authors present a platform for site-specific and multiple-site conjugation to both termini and internal sites of Cas9, and they apply this platform to efficiently engineer insulin-producing β cells.Donghyun LimVedagopuram SreekanthKurt J. CoxBenjamin K. LawBridget K. WagnerJeffrey M. KarpAmit ChoudharyNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Donghyun Lim Vedagopuram Sreekanth Kurt J. Cox Benjamin K. Law Bridget K. Wagner Jeffrey M. Karp Amit Choudhary Engineering designer beta cells with a CRISPR-Cas9 conjugation platform
description	Cas9 fusions partners are often limited to natural polypeptide chains at the Cas9 termni. Here the authors present a platform for site-specific and multiple-site conjugation to both termini and internal sites of Cas9, and they apply this platform to efficiently engineer insulin-producing β cells.
format	article
author	Donghyun Lim Vedagopuram Sreekanth Kurt J. Cox Benjamin K. Law Bridget K. Wagner Jeffrey M. Karp Amit Choudhary
author_facet	Donghyun Lim Vedagopuram Sreekanth Kurt J. Cox Benjamin K. Law Bridget K. Wagner Jeffrey M. Karp Amit Choudhary
author_sort	Donghyun Lim
title	Engineering designer beta cells with a CRISPR-Cas9 conjugation platform
title_short	Engineering designer beta cells with a CRISPR-Cas9 conjugation platform
title_full	Engineering designer beta cells with a CRISPR-Cas9 conjugation platform
title_fullStr	Engineering designer beta cells with a CRISPR-Cas9 conjugation platform
title_full_unstemmed	Engineering designer beta cells with a CRISPR-Cas9 conjugation platform
title_sort	engineering designer beta cells with a crispr-cas9 conjugation platform
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/4fe0e563f9774b8ea8aa70b031449f72
work_keys_str_mv	AT donghyunlim engineeringdesignerbetacellswithacrisprcas9conjugationplatform AT vedagopuramsreekanth engineeringdesignerbetacellswithacrisprcas9conjugationplatform AT kurtjcox engineeringdesignerbetacellswithacrisprcas9conjugationplatform AT benjaminklaw engineeringdesignerbetacellswithacrisprcas9conjugationplatform AT bridgetkwagner engineeringdesignerbetacellswithacrisprcas9conjugationplatform AT jeffreymkarp engineeringdesignerbetacellswithacrisprcas9conjugationplatform AT amitchoudhary engineeringdesignerbetacellswithacrisprcas9conjugationplatform
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Engineering designer beta cells with a CRISPR-Cas9 conjugation platform

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