The Overexpression of <i>NUC</i> Promotes Development and Increases Resistance to Nitrogen Deficiency in <i>Arabidopsis thaliana</i>
NUTCRACKER (NUC) is a transcription factor expressed in multiple tissues, but little is known about its physiological roles. In this study, we explored the physiological function of <i>NUC</i> with the <i>Arabidopsis</i> knockout, rescue, and overexpression lines. We found th...
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| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/4fe59df2f4a34044a249d4ad3d8ad640 |
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| Sumario: | NUTCRACKER (NUC) is a transcription factor expressed in multiple tissues, but little is known about its physiological roles. In this study, we explored the physiological function of <i>NUC</i> with the <i>Arabidopsis</i> knockout, rescue, and overexpression lines. We found that <i>NUC</i> overexpression promoted development at the germination, seedling, and juvenile stages. <i>NUC</i> overexpression increased resistance to nitrogen (N) deficiency stress by increasing the chlorophyll content, suppressing anthocyanin accumulation, and increasing the biomass under N deficiency. In contrast, the absence of <i>NUC</i> did not affect such characteristics. N deficiency significantly increased the expression of <i>NUC</i> in leaves but did not affect the expression of <i>NUC</i> in roots. The overexpression of <i>NUC</i> promoted primary root length under both normal and N deficiency conditions. Furthermore, we found that the N-responsive and lateral-root-related genes <i>TGA1</i> and <i>NRT2.4</i> had NUC-binding sites in their promoter regions and that their expression was upregulated by <i>NUC</i> under N deficiency. The overexpression of the <i>NUC</i> increased the number and length of the lateral roots under N deficiency through inducible promotion. Multiple lines of investigation suggest that the regulatory function of the NUC could be bypassed through its redundant MAGPIE (MGP) when the NUC is absent. Our findings provide novel insight into NUC’s functions and will assist efforts to improve plants’ development and resistance to nutrient stresses. |
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