The Overexpression of NUC Promotes Development and Increases Resistance to Nitrogen Deficiency in Arabidopsis thaliana

NUTCRACKER (NUC) is a transcription factor expressed in multiple tissues, but little is known about its physiological roles. In this study, we explored the physiological function of NUC with the Arabidopsis knockout, rescue, and overexpression lines. We found th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jing Ling, Xing Huang, Yanxia Jia, Weiqi Li, Xudong Zhang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	NUTCRACKER development primary roots lateral roots nitrogen response gene nitrogen deficiency Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/4fe59df2f4a34044a249d4ad3d8ad640
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4fe59df2f4a34044a249d4ad3d8ad640
record_format	dspace
spelling	oai:doaj.org-article:4fe59df2f4a34044a249d4ad3d8ad6402021-11-11T16:53:09ZThe Overexpression of <i>NUC</i> Promotes Development and Increases Resistance to Nitrogen Deficiency in <i>Arabidopsis thaliana</i>10.3390/ijms2221114131422-00671661-6596https://doaj.org/article/4fe59df2f4a34044a249d4ad3d8ad6402021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11413https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067NUTCRACKER (NUC) is a transcription factor expressed in multiple tissues, but little is known about its physiological roles. In this study, we explored the physiological function of <i>NUC</i> with the <i>Arabidopsis</i> knockout, rescue, and overexpression lines. We found that <i>NUC</i> overexpression promoted development at the germination, seedling, and juvenile stages. <i>NUC</i> overexpression increased resistance to nitrogen (N) deficiency stress by increasing the chlorophyll content, suppressing anthocyanin accumulation, and increasing the biomass under N deficiency. In contrast, the absence of <i>NUC</i> did not affect such characteristics. N deficiency significantly increased the expression of <i>NUC</i> in leaves but did not affect the expression of <i>NUC</i> in roots. The overexpression of <i>NUC</i> promoted primary root length under both normal and N deficiency conditions. Furthermore, we found that the N-responsive and lateral-root-related genes <i>TGA1</i> and <i>NRT2.4</i> had NUC-binding sites in their promoter regions and that their expression was upregulated by <i>NUC</i> under N deficiency. The overexpression of the <i>NUC</i> increased the number and length of the lateral roots under N deficiency through inducible promotion. Multiple lines of investigation suggest that the regulatory function of the NUC could be bypassed through its redundant MAGPIE (MGP) when the NUC is absent. Our findings provide novel insight into NUC’s functions and will assist efforts to improve plants’ development and resistance to nutrient stresses.Jing LingXing HuangYanxia JiaWeiqi LiXudong ZhangMDPI AGarticleNUTCRACKERdevelopmentprimary rootslateral rootsnitrogen response genenitrogen deficiencyBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11413, p 11413 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	NUTCRACKER development primary roots lateral roots nitrogen response gene nitrogen deficiency Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	NUTCRACKER development primary roots lateral roots nitrogen response gene nitrogen deficiency Biology (General) QH301-705.5 Chemistry QD1-999 Jing Ling Xing Huang Yanxia Jia Weiqi Li Xudong Zhang The Overexpression of <i>NUC</i> Promotes Development and Increases Resistance to Nitrogen Deficiency in <i>Arabidopsis thaliana</i>
description	NUTCRACKER (NUC) is a transcription factor expressed in multiple tissues, but little is known about its physiological roles. In this study, we explored the physiological function of <i>NUC</i> with the <i>Arabidopsis</i> knockout, rescue, and overexpression lines. We found that <i>NUC</i> overexpression promoted development at the germination, seedling, and juvenile stages. <i>NUC</i> overexpression increased resistance to nitrogen (N) deficiency stress by increasing the chlorophyll content, suppressing anthocyanin accumulation, and increasing the biomass under N deficiency. In contrast, the absence of <i>NUC</i> did not affect such characteristics. N deficiency significantly increased the expression of <i>NUC</i> in leaves but did not affect the expression of <i>NUC</i> in roots. The overexpression of <i>NUC</i> promoted primary root length under both normal and N deficiency conditions. Furthermore, we found that the N-responsive and lateral-root-related genes <i>TGA1</i> and <i>NRT2.4</i> had NUC-binding sites in their promoter regions and that their expression was upregulated by <i>NUC</i> under N deficiency. The overexpression of the <i>NUC</i> increased the number and length of the lateral roots under N deficiency through inducible promotion. Multiple lines of investigation suggest that the regulatory function of the NUC could be bypassed through its redundant MAGPIE (MGP) when the NUC is absent. Our findings provide novel insight into NUC’s functions and will assist efforts to improve plants’ development and resistance to nutrient stresses.
format	article
author	Jing Ling Xing Huang Yanxia Jia Weiqi Li Xudong Zhang
author_facet	Jing Ling Xing Huang Yanxia Jia Weiqi Li Xudong Zhang
author_sort	Jing Ling
title	The Overexpression of <i>NUC</i> Promotes Development and Increases Resistance to Nitrogen Deficiency in <i>Arabidopsis thaliana</i>
title_short	The Overexpression of <i>NUC</i> Promotes Development and Increases Resistance to Nitrogen Deficiency in <i>Arabidopsis thaliana</i>
title_full	The Overexpression of <i>NUC</i> Promotes Development and Increases Resistance to Nitrogen Deficiency in <i>Arabidopsis thaliana</i>
title_fullStr	The Overexpression of <i>NUC</i> Promotes Development and Increases Resistance to Nitrogen Deficiency in <i>Arabidopsis thaliana</i>
title_full_unstemmed	The Overexpression of <i>NUC</i> Promotes Development and Increases Resistance to Nitrogen Deficiency in <i>Arabidopsis thaliana</i>
title_sort	overexpression of <i>nuc</i> promotes development and increases resistance to nitrogen deficiency in <i>arabidopsis thaliana</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4fe59df2f4a34044a249d4ad3d8ad640
work_keys_str_mv	AT jingling theoverexpressionofinucipromotesdevelopmentandincreasesresistancetonitrogendeficiencyiniarabidopsisthalianai AT xinghuang theoverexpressionofinucipromotesdevelopmentandincreasesresistancetonitrogendeficiencyiniarabidopsisthalianai AT yanxiajia theoverexpressionofinucipromotesdevelopmentandincreasesresistancetonitrogendeficiencyiniarabidopsisthalianai AT weiqili theoverexpressionofinucipromotesdevelopmentandincreasesresistancetonitrogendeficiencyiniarabidopsisthalianai AT xudongzhang theoverexpressionofinucipromotesdevelopmentandincreasesresistancetonitrogendeficiencyiniarabidopsisthalianai AT jingling overexpressionofinucipromotesdevelopmentandincreasesresistancetonitrogendeficiencyiniarabidopsisthalianai AT xinghuang overexpressionofinucipromotesdevelopmentandincreasesresistancetonitrogendeficiencyiniarabidopsisthalianai AT yanxiajia overexpressionofinucipromotesdevelopmentandincreasesresistancetonitrogendeficiencyiniarabidopsisthalianai AT weiqili overexpressionofinucipromotesdevelopmentandincreasesresistancetonitrogendeficiencyiniarabidopsisthalianai AT xudongzhang overexpressionofinucipromotesdevelopmentandincreasesresistancetonitrogendeficiencyiniarabidopsisthalianai
_version_	1718432237405339648

The Overexpression of <i>NUC</i> Promotes Development and Increases Resistance to Nitrogen Deficiency in <i>Arabidopsis thaliana</i>

Ejemplares similares