A macrophage-T cell coculture model for severe tissue injury-induced T cell death

Summary: A key observation of tissue injury, such as stroke and burn, is a state of systemic immunosuppression characterized by loss of T cells and rise of infections. Here, we present an in vitro model for cell-cell interactions between innate (macrophages) and adaptive (T cells) immune cells. This...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jie Zhu, Jiayu Cao, Arthur Liesz, Stefan Roth
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell Biology Cell culture Cell isolation Health Sciences Immunology Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/4ff160277f19404f995ee76095294b50
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4ff160277f19404f995ee76095294b50
record_format	dspace
spelling	oai:doaj.org-article:4ff160277f19404f995ee76095294b502021-12-04T04:35:59ZA macrophage-T cell coculture model for severe tissue injury-induced T cell death2666-166710.1016/j.xpro.2021.100983https://doaj.org/article/4ff160277f19404f995ee76095294b502021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006894https://doaj.org/toc/2666-1667Summary: A key observation of tissue injury, such as stroke and burn, is a state of systemic immunosuppression characterized by loss of T cells and rise of infections. Here, we present an in vitro model for cell-cell interactions between innate (macrophages) and adaptive (T cells) immune cells. This protocol facilitates bone marrow-derived macrophages (BMDMs) and splenic T cells in a coculture model. The procedure mimics injury-induced T cell death, which is driven by inflammasome activation in macrophages.For complete details on the use and execution of this protocol, please refer to Roth et al. (2021).Jie ZhuJiayu CaoArthur LieszStefan RothElsevierarticleCell BiologyCell cultureCell isolationHealth SciencesImmunologyScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100983- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell Biology Cell culture Cell isolation Health Sciences Immunology Science (General) Q1-390
spellingShingle	Cell Biology Cell culture Cell isolation Health Sciences Immunology Science (General) Q1-390 Jie Zhu Jiayu Cao Arthur Liesz Stefan Roth A macrophage-T cell coculture model for severe tissue injury-induced T cell death
description	Summary: A key observation of tissue injury, such as stroke and burn, is a state of systemic immunosuppression characterized by loss of T cells and rise of infections. Here, we present an in vitro model for cell-cell interactions between innate (macrophages) and adaptive (T cells) immune cells. This protocol facilitates bone marrow-derived macrophages (BMDMs) and splenic T cells in a coculture model. The procedure mimics injury-induced T cell death, which is driven by inflammasome activation in macrophages.For complete details on the use and execution of this protocol, please refer to Roth et al. (2021).
format	article
author	Jie Zhu Jiayu Cao Arthur Liesz Stefan Roth
author_facet	Jie Zhu Jiayu Cao Arthur Liesz Stefan Roth
author_sort	Jie Zhu
title	A macrophage-T cell coculture model for severe tissue injury-induced T cell death
title_short	A macrophage-T cell coculture model for severe tissue injury-induced T cell death
title_full	A macrophage-T cell coculture model for severe tissue injury-induced T cell death
title_fullStr	A macrophage-T cell coculture model for severe tissue injury-induced T cell death
title_full_unstemmed	A macrophage-T cell coculture model for severe tissue injury-induced T cell death
title_sort	macrophage-t cell coculture model for severe tissue injury-induced t cell death
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/4ff160277f19404f995ee76095294b50
work_keys_str_mv	AT jiezhu amacrophagetcellcoculturemodelforseveretissueinjuryinducedtcelldeath AT jiayucao amacrophagetcellcoculturemodelforseveretissueinjuryinducedtcelldeath AT arthurliesz amacrophagetcellcoculturemodelforseveretissueinjuryinducedtcelldeath AT stefanroth amacrophagetcellcoculturemodelforseveretissueinjuryinducedtcelldeath AT jiezhu macrophagetcellcoculturemodelforseveretissueinjuryinducedtcelldeath AT jiayucao macrophagetcellcoculturemodelforseveretissueinjuryinducedtcelldeath AT arthurliesz macrophagetcellcoculturemodelforseveretissueinjuryinducedtcelldeath AT stefanroth macrophagetcellcoculturemodelforseveretissueinjuryinducedtcelldeath
_version_	1718372910447460352

A macrophage-T cell coculture model for severe tissue injury-induced T cell death

Ejemplares similares