Cellular electron microscopy imaging reveals the localization of the Hfq protein close to the bacterial membrane.

<h4>Background</h4>Hfq is a bacterial protein involved in several aspects of nucleic acid transactions, but one of its best-characterized functions is to affect the post-transcriptional regulation of mRNA by virtue of its interactions with stress-related small regulatory (sRNA).<h4>...

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Autores principales:	Elia Diestra, Bastien Cayrol, Véronique Arluison, Cristina Risco
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4ff20418e85a4d95a3a65443008e19a3
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spelling	oai:doaj.org-article:4ff20418e85a4d95a3a65443008e19a32021-11-25T06:27:26ZCellular electron microscopy imaging reveals the localization of the Hfq protein close to the bacterial membrane.1932-620310.1371/journal.pone.0008301https://doaj.org/article/4ff20418e85a4d95a3a65443008e19a32009-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20011543/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Hfq is a bacterial protein involved in several aspects of nucleic acid transactions, but one of its best-characterized functions is to affect the post-transcriptional regulation of mRNA by virtue of its interactions with stress-related small regulatory (sRNA).<h4>Methodology and principal finding</h4>By using cellular imaging based on the metallothionein clonable tag for electron microscopy, we demonstrate here that in addition to its localization in the cytoplasm and in the nucleoid, a significant amount of Hfq protein is located at the cell periphery. Simultaneous immunogold detection of specific markers strongly suggests that peripheral Hfq is close to the bacterial membrane. Because sRNAs regulate the synthesis of several membrane proteins, our result implies that the sRNA- and Hfq-dependent translational regulation of these proteins takes place in the cytoplasmic region underlying the membrane.<h4>Conclusions</h4>This finding supports the proposal that RNA processing and translational machineries dedicated to membrane protein translation may often be located in close proximity to the membrane of the bacterial cell.Elia DiestraBastien CayrolVéronique ArluisonCristina RiscoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 12, p e8301 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elia Diestra Bastien Cayrol Véronique Arluison Cristina Risco Cellular electron microscopy imaging reveals the localization of the Hfq protein close to the bacterial membrane.
description	<h4>Background</h4>Hfq is a bacterial protein involved in several aspects of nucleic acid transactions, but one of its best-characterized functions is to affect the post-transcriptional regulation of mRNA by virtue of its interactions with stress-related small regulatory (sRNA).<h4>Methodology and principal finding</h4>By using cellular imaging based on the metallothionein clonable tag for electron microscopy, we demonstrate here that in addition to its localization in the cytoplasm and in the nucleoid, a significant amount of Hfq protein is located at the cell periphery. Simultaneous immunogold detection of specific markers strongly suggests that peripheral Hfq is close to the bacterial membrane. Because sRNAs regulate the synthesis of several membrane proteins, our result implies that the sRNA- and Hfq-dependent translational regulation of these proteins takes place in the cytoplasmic region underlying the membrane.<h4>Conclusions</h4>This finding supports the proposal that RNA processing and translational machineries dedicated to membrane protein translation may often be located in close proximity to the membrane of the bacterial cell.
format	article
author	Elia Diestra Bastien Cayrol Véronique Arluison Cristina Risco
author_facet	Elia Diestra Bastien Cayrol Véronique Arluison Cristina Risco
author_sort	Elia Diestra
title	Cellular electron microscopy imaging reveals the localization of the Hfq protein close to the bacterial membrane.
title_short	Cellular electron microscopy imaging reveals the localization of the Hfq protein close to the bacterial membrane.
title_full	Cellular electron microscopy imaging reveals the localization of the Hfq protein close to the bacterial membrane.
title_fullStr	Cellular electron microscopy imaging reveals the localization of the Hfq protein close to the bacterial membrane.
title_full_unstemmed	Cellular electron microscopy imaging reveals the localization of the Hfq protein close to the bacterial membrane.
title_sort	cellular electron microscopy imaging reveals the localization of the hfq protein close to the bacterial membrane.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/4ff20418e85a4d95a3a65443008e19a3
work_keys_str_mv	AT eliadiestra cellularelectronmicroscopyimagingrevealsthelocalizationofthehfqproteinclosetothebacterialmembrane AT bastiencayrol cellularelectronmicroscopyimagingrevealsthelocalizationofthehfqproteinclosetothebacterialmembrane AT veroniquearluison cellularelectronmicroscopyimagingrevealsthelocalizationofthehfqproteinclosetothebacterialmembrane AT cristinarisco cellularelectronmicroscopyimagingrevealsthelocalizationofthehfqproteinclosetothebacterialmembrane
_version_	1718413679452487680

Cellular electron microscopy imaging reveals the localization of the Hfq protein close to the bacterial membrane.

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