Feasibility and effectiveness of endoscopic irreversible electroporation for the upper gastrointestinal tract: an experimental animal study

Abstract Irreversible electroporation (IRE) is a local non-thermal ablative technique currently used to treat solid tumors. Here, we investigated the clinical potency and safety of IRE with an endoscope in the upper gastrointestinal tract. Pigs were electroporated with recently designed endoscopic I...

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Autores principales:	Han Jo Jeon, Hyuk Soon Choi, Bora Keum, Eun Joo Bang, Kang Won Lee, Sang Hyun Kim, Sun Young Yim, Jae Min Lee, Eun Sun Kim, Yeon Seok Seo, Yoon Tae Jeen, Hong Sik Lee, Hoon Jai Chun, Hong Bae Kim, Jong Hyuk Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4ff68d7f73db479f826f88f80bd2c934
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spelling	oai:doaj.org-article:4ff68d7f73db479f826f88f80bd2c9342021-12-02T18:47:07ZFeasibility and effectiveness of endoscopic irreversible electroporation for the upper gastrointestinal tract: an experimental animal study10.1038/s41598-021-94583-w2045-2322https://doaj.org/article/4ff68d7f73db479f826f88f80bd2c9342021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94583-whttps://doaj.org/toc/2045-2322Abstract Irreversible electroporation (IRE) is a local non-thermal ablative technique currently used to treat solid tumors. Here, we investigated the clinical potency and safety of IRE with an endoscope in the upper gastrointestinal tract. Pigs were electroporated with recently designed endoscopic IRE catheters in the esophagus, stomach, and duodenum. Two successive strategies were introduced to optimize the electrical energy for the digestive tract. First, each organ was electroporated and the energy upscaled to confirm the upper limit energy inducing improper tissue results, including bleeding and perforation. Excluding the unacceptable energy from the first step, consecutive electroporations were performed with stepwise reductions in energy to identify the energy that damaged each layer. Inceptive research into inappropriate electrical intensity contributed to extensive hemorrhage and bowel perforation for each tissue above a certain energy threshold. However, experiments performed below the precluded energy accompanying hematoxylin and eosin staining and terminal deoxynucleotidyl transferase dUTP nick-end labeling assays showed that damaged mucosal area and depth significantly decreased with decreased energy. Relevant histopathology showed infiltration of inflammatory cells with pyknotic nuclei at the electroporated lesion. This investigation demonstrated the possibility of endoscopic IRE in mucosal dysplasia or early malignant tumors of the hollow viscus.Han Jo JeonHyuk Soon ChoiBora KeumEun Joo BangKang Won LeeSang Hyun KimSun Young YimJae Min LeeEun Sun KimYeon Seok SeoYoon Tae JeenHong Sik LeeHoon Jai ChunHong Bae KimJong Hyuk KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Han Jo Jeon Hyuk Soon Choi Bora Keum Eun Joo Bang Kang Won Lee Sang Hyun Kim Sun Young Yim Jae Min Lee Eun Sun Kim Yeon Seok Seo Yoon Tae Jeen Hong Sik Lee Hoon Jai Chun Hong Bae Kim Jong Hyuk Kim Feasibility and effectiveness of endoscopic irreversible electroporation for the upper gastrointestinal tract: an experimental animal study
description	Abstract Irreversible electroporation (IRE) is a local non-thermal ablative technique currently used to treat solid tumors. Here, we investigated the clinical potency and safety of IRE with an endoscope in the upper gastrointestinal tract. Pigs were electroporated with recently designed endoscopic IRE catheters in the esophagus, stomach, and duodenum. Two successive strategies were introduced to optimize the electrical energy for the digestive tract. First, each organ was electroporated and the energy upscaled to confirm the upper limit energy inducing improper tissue results, including bleeding and perforation. Excluding the unacceptable energy from the first step, consecutive electroporations were performed with stepwise reductions in energy to identify the energy that damaged each layer. Inceptive research into inappropriate electrical intensity contributed to extensive hemorrhage and bowel perforation for each tissue above a certain energy threshold. However, experiments performed below the precluded energy accompanying hematoxylin and eosin staining and terminal deoxynucleotidyl transferase dUTP nick-end labeling assays showed that damaged mucosal area and depth significantly decreased with decreased energy. Relevant histopathology showed infiltration of inflammatory cells with pyknotic nuclei at the electroporated lesion. This investigation demonstrated the possibility of endoscopic IRE in mucosal dysplasia or early malignant tumors of the hollow viscus.
format	article
author	Han Jo Jeon Hyuk Soon Choi Bora Keum Eun Joo Bang Kang Won Lee Sang Hyun Kim Sun Young Yim Jae Min Lee Eun Sun Kim Yeon Seok Seo Yoon Tae Jeen Hong Sik Lee Hoon Jai Chun Hong Bae Kim Jong Hyuk Kim
author_facet	Han Jo Jeon Hyuk Soon Choi Bora Keum Eun Joo Bang Kang Won Lee Sang Hyun Kim Sun Young Yim Jae Min Lee Eun Sun Kim Yeon Seok Seo Yoon Tae Jeen Hong Sik Lee Hoon Jai Chun Hong Bae Kim Jong Hyuk Kim
author_sort	Han Jo Jeon
title	Feasibility and effectiveness of endoscopic irreversible electroporation for the upper gastrointestinal tract: an experimental animal study
title_short	Feasibility and effectiveness of endoscopic irreversible electroporation for the upper gastrointestinal tract: an experimental animal study
title_full	Feasibility and effectiveness of endoscopic irreversible electroporation for the upper gastrointestinal tract: an experimental animal study
title_fullStr	Feasibility and effectiveness of endoscopic irreversible electroporation for the upper gastrointestinal tract: an experimental animal study
title_full_unstemmed	Feasibility and effectiveness of endoscopic irreversible electroporation for the upper gastrointestinal tract: an experimental animal study
title_sort	feasibility and effectiveness of endoscopic irreversible electroporation for the upper gastrointestinal tract: an experimental animal study
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4ff68d7f73db479f826f88f80bd2c934
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Feasibility and effectiveness of endoscopic irreversible electroporation for the upper gastrointestinal tract: an experimental animal study

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