COMPARATIVE IN- VITRO EVALUATION OF VANCOMYCIN MINIMUM INHIBITORY CONCENTRATIONBY AGAR DILUTION AND E-STRIP IN METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS

Objective: To compare the in-vitro efficacy by determining Minimum Inhibitory Concentration of Vancomycin using the reference Agar Dilution to the E-Strip in Methicillin Resistant Staphylococcus aureus isolates. Study Design: Validation study. Place and Duration of Study: The department of Mic...

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Autores principales:	Sheroze Ilyas, Tehmina Munir, Rabia Sadaf, Mehreen Gilani
Formato:	article
Lenguaje:	EN
Publicado:	Army Medical College Rawalpindi 2021
Materias:	agar dilution e-strip minimum inhibitory concentration methicillin resistant staphylococcus aureus vancomycin intermediate staphylococcus aureus vancomycin resistant staphylococcus aureus Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4ffa139a81404e2994651e99e614e51a
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spelling	oai:doaj.org-article:4ffa139a81404e2994651e99e614e51a2021-12-02T17:10:37ZCOMPARATIVE IN- VITRO EVALUATION OF VANCOMYCIN MINIMUM INHIBITORY CONCENTRATIONBY AGAR DILUTION AND E-STRIP IN METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS0030-96482411-884210.51253/pafmj.v71i1.3529https://doaj.org/article/4ffa139a81404e2994651e99e614e51a2021-02-01T00:00:00Zhttps://pafmj.org/index.php/PAFMJ/article/view/3529https://doaj.org/toc/0030-9648https://doaj.org/toc/2411-8842Objective: To compare the in-vitro efficacy by determining Minimum Inhibitory Concentration of Vancomycin using the reference Agar Dilution to the E-Strip in Methicillin Resistant Staphylococcus aureus isolates. Study Design: Validation study. Place and Duration of Study: The department of Microbiology Army Medical College/National University of Medical Sciences in collaboration with Pak Emirates Military Hospital Rawalpindi, from Dec 2016 to Dec 2017. Methodology: Non-duplicate 84 isolates of Methicillin resistant Staphylococcus aureus from various clinical specimens were included in the study. All these isolates were screened for susceptibility to glycopeptide by E-strips method (Bio mérieux) as well as Agar Dilution method, using vancomycin concentrations of 0.25, 0.50, 1.00, 2.00, 4.00 and 8.00µgm/ml respectively in two fold serial dilutions. Results: There was an overall agreement on 83 samples by both the methods i.e. 83 were Vancomycin Sensitive Staphylococcus aureus by both methods while one isolate with intermediate resistance to Vancomycin was only detected by Agar Dilution. The sensitivity of the E–strips compared to Agar Dilution was found to be 100%. The positive predictive value was 98.8% with a diagnostic accuracy of 98.8%. Specificity and negative predictive value could not be ascertained for E-strips because of the limitation of the method to detect the Vancomycin Intermediate Staphylococcus aureus isolates. Conclusion: E-strip can be a convenient alternative to the gold standard Agar Dilution but its inability to identify VISA challenges its reliability in determining the Vancomycin resistance in MRSA isolates.Sheroze IlyasTehmina MunirRabia SadafMehreen GilaniArmy Medical College Rawalpindiarticleagar dilutione-stripminimum inhibitory concentrationmethicillin resistant staphylococcus aureusvancomycin intermediate staphylococcus aureusvancomycin resistant staphylococcus aureusMedicineRMedicine (General)R5-920ENPakistan Armed Forces Medical Journal, Vol 71, Iss 1, Pp 150-54 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	agar dilution e-strip minimum inhibitory concentration methicillin resistant staphylococcus aureus vancomycin intermediate staphylococcus aureus vancomycin resistant staphylococcus aureus Medicine R Medicine (General) R5-920
spellingShingle	agar dilution e-strip minimum inhibitory concentration methicillin resistant staphylococcus aureus vancomycin intermediate staphylococcus aureus vancomycin resistant staphylococcus aureus Medicine R Medicine (General) R5-920 Sheroze Ilyas Tehmina Munir Rabia Sadaf Mehreen Gilani COMPARATIVE IN- VITRO EVALUATION OF VANCOMYCIN MINIMUM INHIBITORY CONCENTRATIONBY AGAR DILUTION AND E-STRIP IN METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS
description	Objective: To compare the in-vitro efficacy by determining Minimum Inhibitory Concentration of Vancomycin using the reference Agar Dilution to the E-Strip in Methicillin Resistant Staphylococcus aureus isolates. Study Design: Validation study. Place and Duration of Study: The department of Microbiology Army Medical College/National University of Medical Sciences in collaboration with Pak Emirates Military Hospital Rawalpindi, from Dec 2016 to Dec 2017. Methodology: Non-duplicate 84 isolates of Methicillin resistant Staphylococcus aureus from various clinical specimens were included in the study. All these isolates were screened for susceptibility to glycopeptide by E-strips method (Bio mérieux) as well as Agar Dilution method, using vancomycin concentrations of 0.25, 0.50, 1.00, 2.00, 4.00 and 8.00µgm/ml respectively in two fold serial dilutions. Results: There was an overall agreement on 83 samples by both the methods i.e. 83 were Vancomycin Sensitive Staphylococcus aureus by both methods while one isolate with intermediate resistance to Vancomycin was only detected by Agar Dilution. The sensitivity of the E–strips compared to Agar Dilution was found to be 100%. The positive predictive value was 98.8% with a diagnostic accuracy of 98.8%. Specificity and negative predictive value could not be ascertained for E-strips because of the limitation of the method to detect the Vancomycin Intermediate Staphylococcus aureus isolates. Conclusion: E-strip can be a convenient alternative to the gold standard Agar Dilution but its inability to identify VISA challenges its reliability in determining the Vancomycin resistance in MRSA isolates.
format	article
author	Sheroze Ilyas Tehmina Munir Rabia Sadaf Mehreen Gilani
author_facet	Sheroze Ilyas Tehmina Munir Rabia Sadaf Mehreen Gilani
author_sort	Sheroze Ilyas
title	COMPARATIVE IN- VITRO EVALUATION OF VANCOMYCIN MINIMUM INHIBITORY CONCENTRATIONBY AGAR DILUTION AND E-STRIP IN METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS
title_short	COMPARATIVE IN- VITRO EVALUATION OF VANCOMYCIN MINIMUM INHIBITORY CONCENTRATIONBY AGAR DILUTION AND E-STRIP IN METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS
title_full	COMPARATIVE IN- VITRO EVALUATION OF VANCOMYCIN MINIMUM INHIBITORY CONCENTRATIONBY AGAR DILUTION AND E-STRIP IN METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS
title_fullStr	COMPARATIVE IN- VITRO EVALUATION OF VANCOMYCIN MINIMUM INHIBITORY CONCENTRATIONBY AGAR DILUTION AND E-STRIP IN METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS
title_full_unstemmed	COMPARATIVE IN- VITRO EVALUATION OF VANCOMYCIN MINIMUM INHIBITORY CONCENTRATIONBY AGAR DILUTION AND E-STRIP IN METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS
title_sort	comparative in- vitro evaluation of vancomycin minimum inhibitory concentrationby agar dilution and e-strip in methicillin resistant staphylococcus aureus
publisher	Army Medical College Rawalpindi
publishDate	2021
url	https://doaj.org/article/4ffa139a81404e2994651e99e614e51a
work_keys_str_mv	AT sherozeilyas comparativeinvitroevaluationofvancomycinminimuminhibitoryconcentrationbyagardilutionandestripinmethicillinresistantstaphylococcusaureus AT tehminamunir comparativeinvitroevaluationofvancomycinminimuminhibitoryconcentrationbyagardilutionandestripinmethicillinresistantstaphylococcusaureus AT rabiasadaf comparativeinvitroevaluationofvancomycinminimuminhibitoryconcentrationbyagardilutionandestripinmethicillinresistantstaphylococcusaureus AT mehreengilani comparativeinvitroevaluationofvancomycinminimuminhibitoryconcentrationbyagardilutionandestripinmethicillinresistantstaphylococcusaureus
_version_	1718381497744883712

COMPARATIVE IN- VITRO EVALUATION OF VANCOMYCIN MINIMUM INHIBITORY CONCENTRATIONBY AGAR DILUTION AND E-STRIP IN METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS

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