Genomic anatomy of male-specific microchromosomes in a gynogenetic fish.

Unisexual taxa are commonly considered short-lived as the absence of meiotic recombination is supposed to accumulate deleterious mutations and hinder the creation of genetic diversity. However, the gynogenetic gibel carp (Carassius gibelio) with high genetic diversity and wide ecological distributio...

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Autores principales:	Miao Ding, Xi-Yin Li, Zhi-Xuan Zhu, Jun-Hui Chen, Meng Lu, Qian Shi, Yang Wang, Zhi Li, Xin Zhao, Tao Wang, Wen-Xuan Du, Chun Miao, Tian-Zi Yao, Ming-Tao Wang, Xiao-Juan Zhang, Zhong-Wei Wang, Li Zhou, Jian-Fang Gui
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/4ffe9a70388c4d5f9c3b438dc649e0db
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spelling	oai:doaj.org-article:4ffe9a70388c4d5f9c3b438dc649e0db2021-12-02T20:03:21ZGenomic anatomy of male-specific microchromosomes in a gynogenetic fish.1553-73901553-740410.1371/journal.pgen.1009760https://doaj.org/article/4ffe9a70388c4d5f9c3b438dc649e0db2021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009760https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Unisexual taxa are commonly considered short-lived as the absence of meiotic recombination is supposed to accumulate deleterious mutations and hinder the creation of genetic diversity. However, the gynogenetic gibel carp (Carassius gibelio) with high genetic diversity and wide ecological distribution has outlived its predicted extinction time of a strict unisexual reproduction population. Unlike other unisexual vertebrates, males associated with supernumerary microchromosomes have been observed in gibel carp, which provides a unique system to explore the rationales underlying male occurrence in unisexual lineage and evolution of unisexual reproduction. Here, we identified a massively expanded satellite DNA cluster on microchromosomes of hexaploid gibel carp via comparing with the ancestral tetraploid crucian carp (Carassius auratus). Based on the satellite cluster, we developed a method for single chromosomal fluorescence microdissection and isolated three male-specific microchromosomes in a male metaphase cell. Genomic anatomy revealed that these male-specific microchromosomes contained homologous sequences of autosomes and abundant repetitive elements. Significantly, several potential male-specific genes with transcriptional activity were identified, among which four and five genes displayed male-specific and male-biased expression in gonads, respectively, during the developmental period of sex determination. Therefore, the male-specific microchromosomes resembling common features of sex chromosomes may be the main driving force for male occurrence in gynogenetic gibel carp, which sheds new light on the evolution of unisexual reproduction.Miao DingXi-Yin LiZhi-Xuan ZhuJun-Hui ChenMeng LuQian ShiYang WangZhi LiXin ZhaoTao WangWen-Xuan DuChun MiaoTian-Zi YaoMing-Tao WangXiao-Juan ZhangZhong-Wei WangLi ZhouJian-Fang GuiPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 9, p e1009760 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Miao Ding Xi-Yin Li Zhi-Xuan Zhu Jun-Hui Chen Meng Lu Qian Shi Yang Wang Zhi Li Xin Zhao Tao Wang Wen-Xuan Du Chun Miao Tian-Zi Yao Ming-Tao Wang Xiao-Juan Zhang Zhong-Wei Wang Li Zhou Jian-Fang Gui Genomic anatomy of male-specific microchromosomes in a gynogenetic fish.
description	Unisexual taxa are commonly considered short-lived as the absence of meiotic recombination is supposed to accumulate deleterious mutations and hinder the creation of genetic diversity. However, the gynogenetic gibel carp (Carassius gibelio) with high genetic diversity and wide ecological distribution has outlived its predicted extinction time of a strict unisexual reproduction population. Unlike other unisexual vertebrates, males associated with supernumerary microchromosomes have been observed in gibel carp, which provides a unique system to explore the rationales underlying male occurrence in unisexual lineage and evolution of unisexual reproduction. Here, we identified a massively expanded satellite DNA cluster on microchromosomes of hexaploid gibel carp via comparing with the ancestral tetraploid crucian carp (Carassius auratus). Based on the satellite cluster, we developed a method for single chromosomal fluorescence microdissection and isolated three male-specific microchromosomes in a male metaphase cell. Genomic anatomy revealed that these male-specific microchromosomes contained homologous sequences of autosomes and abundant repetitive elements. Significantly, several potential male-specific genes with transcriptional activity were identified, among which four and five genes displayed male-specific and male-biased expression in gonads, respectively, during the developmental period of sex determination. Therefore, the male-specific microchromosomes resembling common features of sex chromosomes may be the main driving force for male occurrence in gynogenetic gibel carp, which sheds new light on the evolution of unisexual reproduction.
format	article
author	Miao Ding Xi-Yin Li Zhi-Xuan Zhu Jun-Hui Chen Meng Lu Qian Shi Yang Wang Zhi Li Xin Zhao Tao Wang Wen-Xuan Du Chun Miao Tian-Zi Yao Ming-Tao Wang Xiao-Juan Zhang Zhong-Wei Wang Li Zhou Jian-Fang Gui
author_facet	Miao Ding Xi-Yin Li Zhi-Xuan Zhu Jun-Hui Chen Meng Lu Qian Shi Yang Wang Zhi Li Xin Zhao Tao Wang Wen-Xuan Du Chun Miao Tian-Zi Yao Ming-Tao Wang Xiao-Juan Zhang Zhong-Wei Wang Li Zhou Jian-Fang Gui
author_sort	Miao Ding
title	Genomic anatomy of male-specific microchromosomes in a gynogenetic fish.
title_short	Genomic anatomy of male-specific microchromosomes in a gynogenetic fish.
title_full	Genomic anatomy of male-specific microchromosomes in a gynogenetic fish.
title_fullStr	Genomic anatomy of male-specific microchromosomes in a gynogenetic fish.
title_full_unstemmed	Genomic anatomy of male-specific microchromosomes in a gynogenetic fish.
title_sort	genomic anatomy of male-specific microchromosomes in a gynogenetic fish.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/4ffe9a70388c4d5f9c3b438dc649e0db
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Genomic anatomy of male-specific microchromosomes in a gynogenetic fish.

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