Integrative analysis of porcine microRNAome during skeletal muscle development.

Pig is an important agricultural animal for meat production and provides a valuable model for many human diseases. Functional studies have demonstrated that microRNAs (miRNAs) play critical roles in almost all aspects of skeletal muscle development and disease pathogenesis. To investigate the miRNAs...

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Autores principales:	Lijun Qin, Yaosheng Chen, Xiaohong Liu, Sanxing Ye, Kaifan Yu, Zheng Huang, Jingwei Yu, Xingyu Zhou, Hu Chen, Delin Mo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4fffac21b3984421aae2174e3d7ba28e
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spelling	oai:doaj.org-article:4fffac21b3984421aae2174e3d7ba28e2021-11-18T08:55:50ZIntegrative analysis of porcine microRNAome during skeletal muscle development.1932-620310.1371/journal.pone.0072418https://doaj.org/article/4fffac21b3984421aae2174e3d7ba28e2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24039761/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Pig is an important agricultural animal for meat production and provides a valuable model for many human diseases. Functional studies have demonstrated that microRNAs (miRNAs) play critical roles in almost all aspects of skeletal muscle development and disease pathogenesis. To investigate the miRNAs involved in regulating different periods of skeletal muscle development, we herein performed a comprehensive research for porcine microRNAome (miRNAome) during 10 skeletal muscle developmental stages including 35, 49, 63, 77, 91 dpc (days post coitum) and 2, 28, 90, 120, 180 dpn (days postnatal) using Solexa sequencing technology. Our results extend the repertoire of pig miRNAome to 247 known miRNAs processed from 210 pre-miRNAs and 297 candidate novel miRNAs through comparison with known miRNAs in the miRBase. Expression analysis of the 15 most abundant miRNAs in every library indicated that functional miRNAome may be smaller and tend to be highly expressed. A series of muscle-related miRNAs summarized in our study present different patterns between myofibers formation phase and muscle maturation phase, providing valuable reference for investigation of functional miRNAs during skeletal muscle development. Analysis of temporal profiles of miRNA expression identifies 18 novel candidate myogenic miRNAs in pig, which might provide new insight into regulation mechanism mediated by miRNAs underlying muscle development.Lijun QinYaosheng ChenXiaohong LiuSanxing YeKaifan YuZheng HuangJingwei YuXingyu ZhouHu ChenDelin MoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e72418 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lijun Qin Yaosheng Chen Xiaohong Liu Sanxing Ye Kaifan Yu Zheng Huang Jingwei Yu Xingyu Zhou Hu Chen Delin Mo Integrative analysis of porcine microRNAome during skeletal muscle development.
description	Pig is an important agricultural animal for meat production and provides a valuable model for many human diseases. Functional studies have demonstrated that microRNAs (miRNAs) play critical roles in almost all aspects of skeletal muscle development and disease pathogenesis. To investigate the miRNAs involved in regulating different periods of skeletal muscle development, we herein performed a comprehensive research for porcine microRNAome (miRNAome) during 10 skeletal muscle developmental stages including 35, 49, 63, 77, 91 dpc (days post coitum) and 2, 28, 90, 120, 180 dpn (days postnatal) using Solexa sequencing technology. Our results extend the repertoire of pig miRNAome to 247 known miRNAs processed from 210 pre-miRNAs and 297 candidate novel miRNAs through comparison with known miRNAs in the miRBase. Expression analysis of the 15 most abundant miRNAs in every library indicated that functional miRNAome may be smaller and tend to be highly expressed. A series of muscle-related miRNAs summarized in our study present different patterns between myofibers formation phase and muscle maturation phase, providing valuable reference for investigation of functional miRNAs during skeletal muscle development. Analysis of temporal profiles of miRNA expression identifies 18 novel candidate myogenic miRNAs in pig, which might provide new insight into regulation mechanism mediated by miRNAs underlying muscle development.
format	article
author	Lijun Qin Yaosheng Chen Xiaohong Liu Sanxing Ye Kaifan Yu Zheng Huang Jingwei Yu Xingyu Zhou Hu Chen Delin Mo
author_facet	Lijun Qin Yaosheng Chen Xiaohong Liu Sanxing Ye Kaifan Yu Zheng Huang Jingwei Yu Xingyu Zhou Hu Chen Delin Mo
author_sort	Lijun Qin
title	Integrative analysis of porcine microRNAome during skeletal muscle development.
title_short	Integrative analysis of porcine microRNAome during skeletal muscle development.
title_full	Integrative analysis of porcine microRNAome during skeletal muscle development.
title_fullStr	Integrative analysis of porcine microRNAome during skeletal muscle development.
title_full_unstemmed	Integrative analysis of porcine microRNAome during skeletal muscle development.
title_sort	integrative analysis of porcine micrornaome during skeletal muscle development.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/4fffac21b3984421aae2174e3d7ba28e
work_keys_str_mv	AT lijunqin integrativeanalysisofporcinemicrornaomeduringskeletalmuscledevelopment AT yaoshengchen integrativeanalysisofporcinemicrornaomeduringskeletalmuscledevelopment AT xiaohongliu integrativeanalysisofporcinemicrornaomeduringskeletalmuscledevelopment AT sanxingye integrativeanalysisofporcinemicrornaomeduringskeletalmuscledevelopment AT kaifanyu integrativeanalysisofporcinemicrornaomeduringskeletalmuscledevelopment AT zhenghuang integrativeanalysisofporcinemicrornaomeduringskeletalmuscledevelopment AT jingweiyu integrativeanalysisofporcinemicrornaomeduringskeletalmuscledevelopment AT xingyuzhou integrativeanalysisofporcinemicrornaomeduringskeletalmuscledevelopment AT huchen integrativeanalysisofporcinemicrornaomeduringskeletalmuscledevelopment AT delinmo integrativeanalysisofporcinemicrornaomeduringskeletalmuscledevelopment
_version_	1718421139486670848

Integrative analysis of porcine microRNAome during skeletal muscle development.

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