Prevalence and Genetic Relationship of Predominant Escherichia coli Serotypes Isolated from Poultry, Wild Animals, and Environment in the Mekong Delta, Vietnam

Avian pathogenic Escherichia coli (APEC) is the main causative agent of avian colibacillosis, which is an important systemic disease of profound economic and clinical consequences for the poultry industry worldwide. In this study, 975 E. coli strains were isolated from 2,169 samples collected from c...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lam Thanh Nguyen, Nguyen Khanh Thuan, Nguyen Thu Tam, Chau Thi Huyen Trang, Nguyen Phuc Khanh, Tran Ngoc Bich, Takahide Taniguchi, Hideki Hayashidani, Ly Thi Lien Khai
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/500887653c6e4f0386ec5ad7fc9233ad
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:500887653c6e4f0386ec5ad7fc9233ad
record_format	dspace
spelling	oai:doaj.org-article:500887653c6e4f0386ec5ad7fc9233ad2021-11-22T01:11:17ZPrevalence and Genetic Relationship of Predominant Escherichia coli Serotypes Isolated from Poultry, Wild Animals, and Environment in the Mekong Delta, Vietnam2042-004810.1155/2021/6504648https://doaj.org/article/500887653c6e4f0386ec5ad7fc9233ad2021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/6504648https://doaj.org/toc/2042-0048Avian pathogenic Escherichia coli (APEC) is the main causative agent of avian colibacillosis, which is an important systemic disease of profound economic and clinical consequences for the poultry industry worldwide. In this study, 975 E. coli strains were isolated from 2,169 samples collected from cloacal swabs of chickens, in-farm wild animals (ants, geckos, flies, and rats), and environment. The highest proportion of E. coli isolation was obtained from chicken cloacal swabs with 71.05% (95% confidence interval (CI) 66.69–75.05%) followed by the proportions of 38.15% (95% CI 35.41–40.97%) and 38.11% (95% CI 34.15–42.24%) from wild animals or environment, respectively. Distribution of O-antigen serotypes of the E. coli isolates, including O1, O2, O18, and O78, was determined by PCR. The most predominant serotype was O18 (10.56%) followed by O2 (9.44%), O1 (7.79%), and O78 (6.56%). Of note, serotype O18 was more likely distributed in the examined wild animals, especially in geckos. Polymorphic DNA fingerprints, generated by ERIC-PCR, of representative E. coli strains of each serotype revealed genetic heterogeneity of the examined E. coli, and O18 was more divergent with 63 clusters formed from 66 isolates. Furthermore, several E. coli strains from different sample sources shared high DNA fingerprint relatedness, suggesting that there exists complex transmission of E. coli from chickens to wild animals and environment and vice versa in poultry husbandry settings. Although pathotypes of the examined E. coli were not determined in this study, our results provided important findings of epidemiological and genetic characteristics of E. coli in the Mekong Delta and highlighted the prerequisite of stricter biocontainment to reduce the prevalence and consequences of APEC in poultry production.Lam Thanh NguyenNguyen Khanh ThuanNguyen Thu TamChau Thi Huyen TrangNguyen Phuc KhanhTran Ngoc BichTakahide TaniguchiHideki HayashidaniLy Thi Lien KhaiHindawi LimitedarticleVeterinary medicineSF600-1100ENVeterinary Medicine International, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Veterinary medicine SF600-1100
spellingShingle	Veterinary medicine SF600-1100 Lam Thanh Nguyen Nguyen Khanh Thuan Nguyen Thu Tam Chau Thi Huyen Trang Nguyen Phuc Khanh Tran Ngoc Bich Takahide Taniguchi Hideki Hayashidani Ly Thi Lien Khai Prevalence and Genetic Relationship of Predominant Escherichia coli Serotypes Isolated from Poultry, Wild Animals, and Environment in the Mekong Delta, Vietnam
description	Avian pathogenic Escherichia coli (APEC) is the main causative agent of avian colibacillosis, which is an important systemic disease of profound economic and clinical consequences for the poultry industry worldwide. In this study, 975 E. coli strains were isolated from 2,169 samples collected from cloacal swabs of chickens, in-farm wild animals (ants, geckos, flies, and rats), and environment. The highest proportion of E. coli isolation was obtained from chicken cloacal swabs with 71.05% (95% confidence interval (CI) 66.69–75.05%) followed by the proportions of 38.15% (95% CI 35.41–40.97%) and 38.11% (95% CI 34.15–42.24%) from wild animals or environment, respectively. Distribution of O-antigen serotypes of the E. coli isolates, including O1, O2, O18, and O78, was determined by PCR. The most predominant serotype was O18 (10.56%) followed by O2 (9.44%), O1 (7.79%), and O78 (6.56%). Of note, serotype O18 was more likely distributed in the examined wild animals, especially in geckos. Polymorphic DNA fingerprints, generated by ERIC-PCR, of representative E. coli strains of each serotype revealed genetic heterogeneity of the examined E. coli, and O18 was more divergent with 63 clusters formed from 66 isolates. Furthermore, several E. coli strains from different sample sources shared high DNA fingerprint relatedness, suggesting that there exists complex transmission of E. coli from chickens to wild animals and environment and vice versa in poultry husbandry settings. Although pathotypes of the examined E. coli were not determined in this study, our results provided important findings of epidemiological and genetic characteristics of E. coli in the Mekong Delta and highlighted the prerequisite of stricter biocontainment to reduce the prevalence and consequences of APEC in poultry production.
format	article
author	Lam Thanh Nguyen Nguyen Khanh Thuan Nguyen Thu Tam Chau Thi Huyen Trang Nguyen Phuc Khanh Tran Ngoc Bich Takahide Taniguchi Hideki Hayashidani Ly Thi Lien Khai
author_facet	Lam Thanh Nguyen Nguyen Khanh Thuan Nguyen Thu Tam Chau Thi Huyen Trang Nguyen Phuc Khanh Tran Ngoc Bich Takahide Taniguchi Hideki Hayashidani Ly Thi Lien Khai
author_sort	Lam Thanh Nguyen
title	Prevalence and Genetic Relationship of Predominant Escherichia coli Serotypes Isolated from Poultry, Wild Animals, and Environment in the Mekong Delta, Vietnam
title_short	Prevalence and Genetic Relationship of Predominant Escherichia coli Serotypes Isolated from Poultry, Wild Animals, and Environment in the Mekong Delta, Vietnam
title_full	Prevalence and Genetic Relationship of Predominant Escherichia coli Serotypes Isolated from Poultry, Wild Animals, and Environment in the Mekong Delta, Vietnam
title_fullStr	Prevalence and Genetic Relationship of Predominant Escherichia coli Serotypes Isolated from Poultry, Wild Animals, and Environment in the Mekong Delta, Vietnam
title_full_unstemmed	Prevalence and Genetic Relationship of Predominant Escherichia coli Serotypes Isolated from Poultry, Wild Animals, and Environment in the Mekong Delta, Vietnam
title_sort	prevalence and genetic relationship of predominant escherichia coli serotypes isolated from poultry, wild animals, and environment in the mekong delta, vietnam
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/500887653c6e4f0386ec5ad7fc9233ad
work_keys_str_mv	AT lamthanhnguyen prevalenceandgeneticrelationshipofpredominantescherichiacoliserotypesisolatedfrompoultrywildanimalsandenvironmentinthemekongdeltavietnam AT nguyenkhanhthuan prevalenceandgeneticrelationshipofpredominantescherichiacoliserotypesisolatedfrompoultrywildanimalsandenvironmentinthemekongdeltavietnam AT nguyenthutam prevalenceandgeneticrelationshipofpredominantescherichiacoliserotypesisolatedfrompoultrywildanimalsandenvironmentinthemekongdeltavietnam AT chauthihuyentrang prevalenceandgeneticrelationshipofpredominantescherichiacoliserotypesisolatedfrompoultrywildanimalsandenvironmentinthemekongdeltavietnam AT nguyenphuckhanh prevalenceandgeneticrelationshipofpredominantescherichiacoliserotypesisolatedfrompoultrywildanimalsandenvironmentinthemekongdeltavietnam AT tranngocbich prevalenceandgeneticrelationshipofpredominantescherichiacoliserotypesisolatedfrompoultrywildanimalsandenvironmentinthemekongdeltavietnam AT takahidetaniguchi prevalenceandgeneticrelationshipofpredominantescherichiacoliserotypesisolatedfrompoultrywildanimalsandenvironmentinthemekongdeltavietnam AT hidekihayashidani prevalenceandgeneticrelationshipofpredominantescherichiacoliserotypesisolatedfrompoultrywildanimalsandenvironmentinthemekongdeltavietnam AT lythilienkhai prevalenceandgeneticrelationshipofpredominantescherichiacoliserotypesisolatedfrompoultrywildanimalsandenvironmentinthemekongdeltavietnam
_version_	1718418280937422848

Prevalence and Genetic Relationship of Predominant Escherichia coli Serotypes Isolated from Poultry, Wild Animals, and Environment in the Mekong Delta, Vietnam

Ejemplares similares