CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing

Cpf1 is a promising addition to the CRISPR toolkit but displays wide variability of activity in different eurkaryotes. Here the authors identify temperature as a modulator of activity and use this to efficiently edit ectothermic vertebrate species.

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Autores principales:	Miguel A. Moreno-Mateos, Juan P. Fernandez, Romain Rouet, Charles E. Vejnar, Maura A. Lane, Emily Mis, Mustafa K. Khokha, Jennifer A. Doudna, Antonio J. Giraldez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/50088efca89a4745ab6dd5837c5a5dc4
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spelling	oai:doaj.org-article:50088efca89a4745ab6dd5837c5a5dc42021-12-02T14:42:38ZCRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing10.1038/s41467-017-01836-22041-1723https://doaj.org/article/50088efca89a4745ab6dd5837c5a5dc42017-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-01836-2https://doaj.org/toc/2041-1723Cpf1 is a promising addition to the CRISPR toolkit but displays wide variability of activity in different eurkaryotes. Here the authors identify temperature as a modulator of activity and use this to efficiently edit ectothermic vertebrate species.Miguel A. Moreno-MateosJuan P. FernandezRomain RouetCharles E. VejnarMaura A. LaneEmily MisMustafa K. KhokhaJennifer A. DoudnaAntonio J. GiraldezNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Miguel A. Moreno-Mateos Juan P. Fernandez Romain Rouet Charles E. Vejnar Maura A. Lane Emily Mis Mustafa K. Khokha Jennifer A. Doudna Antonio J. Giraldez CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing
description	Cpf1 is a promising addition to the CRISPR toolkit but displays wide variability of activity in different eurkaryotes. Here the authors identify temperature as a modulator of activity and use this to efficiently edit ectothermic vertebrate species.
format	article
author	Miguel A. Moreno-Mateos Juan P. Fernandez Romain Rouet Charles E. Vejnar Maura A. Lane Emily Mis Mustafa K. Khokha Jennifer A. Doudna Antonio J. Giraldez
author_facet	Miguel A. Moreno-Mateos Juan P. Fernandez Romain Rouet Charles E. Vejnar Maura A. Lane Emily Mis Mustafa K. Khokha Jennifer A. Doudna Antonio J. Giraldez
author_sort	Miguel A. Moreno-Mateos
title	CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing
title_short	CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing
title_full	CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing
title_fullStr	CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing
title_full_unstemmed	CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing
title_sort	crispr-cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/50088efca89a4745ab6dd5837c5a5dc4
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CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing

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