Analysis of autofluorescence in polymorphonuclear neutrophils: a new tool for early infection diagnosis.

Diagnosing bacterial infection (BI) remains a challenge for the attending physician. An ex vivo infection model based on human fixed polymorphonuclear neutrophils (PMNs) gives an autofluorescence signal that differs significantly between stimulated and unstimulated cells. We took advantage of this p...

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Autores principales:	Antoine Monsel, Sandrine Lécart, Antoine Roquilly, Alexis Broquet, Cédric Jacqueline, Tristan Mirault, Thibaut Troude, Marie-Pierre Fontaine-Aupart, Karim Asehnoune
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/500c820c8e41406d909d6d625785a641
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spelling	oai:doaj.org-article:500c820c8e41406d909d6d625785a6412021-11-18T08:26:44ZAnalysis of autofluorescence in polymorphonuclear neutrophils: a new tool for early infection diagnosis.1932-620310.1371/journal.pone.0092564https://doaj.org/article/500c820c8e41406d909d6d625785a6412014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24658436/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Diagnosing bacterial infection (BI) remains a challenge for the attending physician. An ex vivo infection model based on human fixed polymorphonuclear neutrophils (PMNs) gives an autofluorescence signal that differs significantly between stimulated and unstimulated cells. We took advantage of this property for use in an in vivo pneumonia mouse model and in patients hospitalized with bacterial pneumonia. A 2-fold decrease was observed in autofluorescence intensity for cytospined PMNs from broncho-alveolar lavage (BAL) in the pneumonia mouse model and a 2.7-fold decrease was observed in patients with pneumonia when compared with control mice or patients without pneumonia, respectively. This optical method provided an autofluorescence mean intensity cut-off, allowing for easy diagnosis of BI. Originally set up on a confocal microscope, the assay was also effective using a standard epifluorescence microscope. Assessing the autofluorescence of PMNs provides a fast, simple, cheap and reliable method optimizing the efficiency and the time needed for early diagnosis of severe infections. Rationalized therapeutic decisions supported by the results from this method can improve the outcome of patients suspected of having an infection.Antoine MonselSandrine LécartAntoine RoquillyAlexis BroquetCédric JacquelineTristan MiraultThibaut TroudeMarie-Pierre Fontaine-AupartKarim AsehnounePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e92564 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Antoine Monsel Sandrine Lécart Antoine Roquilly Alexis Broquet Cédric Jacqueline Tristan Mirault Thibaut Troude Marie-Pierre Fontaine-Aupart Karim Asehnoune Analysis of autofluorescence in polymorphonuclear neutrophils: a new tool for early infection diagnosis.
description	Diagnosing bacterial infection (BI) remains a challenge for the attending physician. An ex vivo infection model based on human fixed polymorphonuclear neutrophils (PMNs) gives an autofluorescence signal that differs significantly between stimulated and unstimulated cells. We took advantage of this property for use in an in vivo pneumonia mouse model and in patients hospitalized with bacterial pneumonia. A 2-fold decrease was observed in autofluorescence intensity for cytospined PMNs from broncho-alveolar lavage (BAL) in the pneumonia mouse model and a 2.7-fold decrease was observed in patients with pneumonia when compared with control mice or patients without pneumonia, respectively. This optical method provided an autofluorescence mean intensity cut-off, allowing for easy diagnosis of BI. Originally set up on a confocal microscope, the assay was also effective using a standard epifluorescence microscope. Assessing the autofluorescence of PMNs provides a fast, simple, cheap and reliable method optimizing the efficiency and the time needed for early diagnosis of severe infections. Rationalized therapeutic decisions supported by the results from this method can improve the outcome of patients suspected of having an infection.
format	article
author	Antoine Monsel Sandrine Lécart Antoine Roquilly Alexis Broquet Cédric Jacqueline Tristan Mirault Thibaut Troude Marie-Pierre Fontaine-Aupart Karim Asehnoune
author_facet	Antoine Monsel Sandrine Lécart Antoine Roquilly Alexis Broquet Cédric Jacqueline Tristan Mirault Thibaut Troude Marie-Pierre Fontaine-Aupart Karim Asehnoune
author_sort	Antoine Monsel
title	Analysis of autofluorescence in polymorphonuclear neutrophils: a new tool for early infection diagnosis.
title_short	Analysis of autofluorescence in polymorphonuclear neutrophils: a new tool for early infection diagnosis.
title_full	Analysis of autofluorescence in polymorphonuclear neutrophils: a new tool for early infection diagnosis.
title_fullStr	Analysis of autofluorescence in polymorphonuclear neutrophils: a new tool for early infection diagnosis.
title_full_unstemmed	Analysis of autofluorescence in polymorphonuclear neutrophils: a new tool for early infection diagnosis.
title_sort	analysis of autofluorescence in polymorphonuclear neutrophils: a new tool for early infection diagnosis.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/500c820c8e41406d909d6d625785a641
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Analysis of autofluorescence in polymorphonuclear neutrophils: a new tool for early infection diagnosis.

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