Single cell deposition and patterning with a robotic system.

Integrating single-cell manipulation techniques in traditional and emerging biological culture systems is challenging. Microfabricated devices for single cell studies in particular often require cells to be spatially positioned at specific culture sites on the device surface. This paper presents a r...

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Autores principales:	Zhe Lu, Christopher Moraes, George Ye, Craig A Simmons, Yu Sun
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/50111027a1f34c128259c4434e76db7f
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spelling	oai:doaj.org-article:50111027a1f34c128259c4434e76db7f2021-11-18T07:03:02ZSingle cell deposition and patterning with a robotic system.1932-620310.1371/journal.pone.0013542https://doaj.org/article/50111027a1f34c128259c4434e76db7f2010-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21042403/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Integrating single-cell manipulation techniques in traditional and emerging biological culture systems is challenging. Microfabricated devices for single cell studies in particular often require cells to be spatially positioned at specific culture sites on the device surface. This paper presents a robotic micromanipulation system for pick-and-place positioning of single cells. By integrating computer vision and motion control algorithms, the system visually tracks a cell in real time and controls multiple positioning devices simultaneously to accurately pick up a single cell, transfer it to a desired substrate, and deposit it at a specified location. A traditional glass micropipette is used, and whole- and partial-cell aspiration techniques are investigated to manipulate single cells. Partially aspirating cells resulted in an operation speed of 15 seconds per cell and a 95% success rate. In contrast, the whole-cell aspiration method required 30 seconds per cell and achieved a success rate of 80%. The broad applicability of this robotic manipulation technique is demonstrated using multiple cell types on traditional substrates and on open-top microfabricated devices, without requiring modifications to device designs. Furthermore, we used this serial deposition process in conjunction with an established parallel cell manipulation technique to improve the efficiency of single cell capture from ∼80% to 100%. Using a robotic micromanipulation system to position single cells on a substrate is demonstrated as an effective stand-alone or bolstering technology for single-cell studies, eliminating some of the drawbacks associated with standard single-cell handling and manipulation techniques.Zhe LuChristopher MoraesGeorge YeCraig A SimmonsYu SunPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 10, p e13542 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhe Lu Christopher Moraes George Ye Craig A Simmons Yu Sun Single cell deposition and patterning with a robotic system.
description	Integrating single-cell manipulation techniques in traditional and emerging biological culture systems is challenging. Microfabricated devices for single cell studies in particular often require cells to be spatially positioned at specific culture sites on the device surface. This paper presents a robotic micromanipulation system for pick-and-place positioning of single cells. By integrating computer vision and motion control algorithms, the system visually tracks a cell in real time and controls multiple positioning devices simultaneously to accurately pick up a single cell, transfer it to a desired substrate, and deposit it at a specified location. A traditional glass micropipette is used, and whole- and partial-cell aspiration techniques are investigated to manipulate single cells. Partially aspirating cells resulted in an operation speed of 15 seconds per cell and a 95% success rate. In contrast, the whole-cell aspiration method required 30 seconds per cell and achieved a success rate of 80%. The broad applicability of this robotic manipulation technique is demonstrated using multiple cell types on traditional substrates and on open-top microfabricated devices, without requiring modifications to device designs. Furthermore, we used this serial deposition process in conjunction with an established parallel cell manipulation technique to improve the efficiency of single cell capture from ∼80% to 100%. Using a robotic micromanipulation system to position single cells on a substrate is demonstrated as an effective stand-alone or bolstering technology for single-cell studies, eliminating some of the drawbacks associated with standard single-cell handling and manipulation techniques.
format	article
author	Zhe Lu Christopher Moraes George Ye Craig A Simmons Yu Sun
author_facet	Zhe Lu Christopher Moraes George Ye Craig A Simmons Yu Sun
author_sort	Zhe Lu
title	Single cell deposition and patterning with a robotic system.
title_short	Single cell deposition and patterning with a robotic system.
title_full	Single cell deposition and patterning with a robotic system.
title_fullStr	Single cell deposition and patterning with a robotic system.
title_full_unstemmed	Single cell deposition and patterning with a robotic system.
title_sort	single cell deposition and patterning with a robotic system.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/50111027a1f34c128259c4434e76db7f
work_keys_str_mv	AT zhelu singlecelldepositionandpatterningwitharoboticsystem AT christophermoraes singlecelldepositionandpatterningwitharoboticsystem AT georgeye singlecelldepositionandpatterningwitharoboticsystem AT craigasimmons singlecelldepositionandpatterningwitharoboticsystem AT yusun singlecelldepositionandpatterningwitharoboticsystem
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Single cell deposition and patterning with a robotic system.

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