Suppression of a Novel Vitellogenesis-Inhibiting Hormone Significantly Increases Ovarian Vitellogenesis in the Black Tiger Shrimp, Penaeus monodon

In this study, a novel Crustacean Hyperglycemic Hormone-type II gene (CHH-type II) was identified and biologically characterized in a shrimp, Penaeus monodon. Based on its structure and function, this gene was named P. monodon vitellogenesis-inhibiting hormone (PemVIH). The complete cDNA sequence of...

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Autores principales:	Phaivit Laphyai, Thanapong Kruangkum, Charoonroj Chotwiwatthanakun, Wanita Semchuchot, Prawporn Thaijongrak, Prasert Sobhon, Pei-San Tsai, Rapeepun Vanichviriyakit
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	crustacean hyperglycemic hormone vitellogenin neurohormone crustacean reproduction Diseases of the endocrine glands. Clinical endocrinology RC648-665
Acceso en línea:	https://doaj.org/article/5012890398ac4ac1a63232ae0a763769
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spelling	oai:doaj.org-article:5012890398ac4ac1a63232ae0a7637692021-11-15T14:48:50ZSuppression of a Novel Vitellogenesis-Inhibiting Hormone Significantly Increases Ovarian Vitellogenesis in the Black Tiger Shrimp, Penaeus monodon1664-239210.3389/fendo.2021.760538https://doaj.org/article/5012890398ac4ac1a63232ae0a7637692021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fendo.2021.760538/fullhttps://doaj.org/toc/1664-2392In this study, a novel Crustacean Hyperglycemic Hormone-type II gene (CHH-type II) was identified and biologically characterized in a shrimp, Penaeus monodon. Based on its structure and function, this gene was named P. monodon vitellogenesis-inhibiting hormone (PemVIH). The complete cDNA sequence of PemVIH consisted of 1,022 nt with an open reading frame (ORF) of 339 nt encoding a polypeptide of 112 amino acids. It was classified as a member of the CHH-type II family based on conserved cysteine residues, a characteristically positioned glycine residue, and the absence of CHH precursor-related peptide (CPRP) domain. The deduced mature PemVIH shared the highest sequence similarities with giant river prawn sinus gland peptide A. Unlike P. monodon gonad-inhibiting hormone (PemGIH), PemVIH was expressed only in the brain and ventral nerve cord, but not the eyestalks. Whole mount immunofluorescence using a newly generated PemVIH antiserum detected positive signals in neuronal cluster 9/11 and 17 of the brain, commissural ganglion (CoG), and neuronal clusters of ventral nerve cord. The presence of PemVIH-positive neurons in CoG, a part of stomatogastric nervous system, suggested a potential mechanism for crosstalk between nutritional and reproductive signaling. The role of PemVIH in vitellogenesis was evaluated using RNA interference technique. Temporal knockdown of PemVIH in female subadults resulted in a 3-fold increase in ovarian vitellogenin expression, suggesting an inhibitory role of PemVIH in vitellogenesis. This study provided novel insight into the control of vitellogenesis and additional strategies for improving ovarian maturation in P. monodon without the current harmful practice of eyestalk ablation.Phaivit LaphyaiPhaivit LaphyaiThanapong KruangkumThanapong KruangkumCharoonroj ChotwiwatthanakunCharoonroj ChotwiwatthanakunWanita SemchuchotWanita SemchuchotPrawporn ThaijongrakPrawporn ThaijongrakPrasert SobhonPei-San TsaiRapeepun VanichviriyakitRapeepun VanichviriyakitFrontiers Media S.A.articlecrustacean hyperglycemic hormonevitellogeninneurohormonecrustaceanreproductionDiseases of the endocrine glands. Clinical endocrinologyRC648-665ENFrontiers in Endocrinology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	crustacean hyperglycemic hormone vitellogenin neurohormone crustacean reproduction Diseases of the endocrine glands. Clinical endocrinology RC648-665
spellingShingle	crustacean hyperglycemic hormone vitellogenin neurohormone crustacean reproduction Diseases of the endocrine glands. Clinical endocrinology RC648-665 Phaivit Laphyai Phaivit Laphyai Thanapong Kruangkum Thanapong Kruangkum Charoonroj Chotwiwatthanakun Charoonroj Chotwiwatthanakun Wanita Semchuchot Wanita Semchuchot Prawporn Thaijongrak Prawporn Thaijongrak Prasert Sobhon Pei-San Tsai Rapeepun Vanichviriyakit Rapeepun Vanichviriyakit Suppression of a Novel Vitellogenesis-Inhibiting Hormone Significantly Increases Ovarian Vitellogenesis in the Black Tiger Shrimp, Penaeus monodon
description	In this study, a novel Crustacean Hyperglycemic Hormone-type II gene (CHH-type II) was identified and biologically characterized in a shrimp, Penaeus monodon. Based on its structure and function, this gene was named P. monodon vitellogenesis-inhibiting hormone (PemVIH). The complete cDNA sequence of PemVIH consisted of 1,022 nt with an open reading frame (ORF) of 339 nt encoding a polypeptide of 112 amino acids. It was classified as a member of the CHH-type II family based on conserved cysteine residues, a characteristically positioned glycine residue, and the absence of CHH precursor-related peptide (CPRP) domain. The deduced mature PemVIH shared the highest sequence similarities with giant river prawn sinus gland peptide A. Unlike P. monodon gonad-inhibiting hormone (PemGIH), PemVIH was expressed only in the brain and ventral nerve cord, but not the eyestalks. Whole mount immunofluorescence using a newly generated PemVIH antiserum detected positive signals in neuronal cluster 9/11 and 17 of the brain, commissural ganglion (CoG), and neuronal clusters of ventral nerve cord. The presence of PemVIH-positive neurons in CoG, a part of stomatogastric nervous system, suggested a potential mechanism for crosstalk between nutritional and reproductive signaling. The role of PemVIH in vitellogenesis was evaluated using RNA interference technique. Temporal knockdown of PemVIH in female subadults resulted in a 3-fold increase in ovarian vitellogenin expression, suggesting an inhibitory role of PemVIH in vitellogenesis. This study provided novel insight into the control of vitellogenesis and additional strategies for improving ovarian maturation in P. monodon without the current harmful practice of eyestalk ablation.
format	article
author	Phaivit Laphyai Phaivit Laphyai Thanapong Kruangkum Thanapong Kruangkum Charoonroj Chotwiwatthanakun Charoonroj Chotwiwatthanakun Wanita Semchuchot Wanita Semchuchot Prawporn Thaijongrak Prawporn Thaijongrak Prasert Sobhon Pei-San Tsai Rapeepun Vanichviriyakit Rapeepun Vanichviriyakit
author_facet	Phaivit Laphyai Phaivit Laphyai Thanapong Kruangkum Thanapong Kruangkum Charoonroj Chotwiwatthanakun Charoonroj Chotwiwatthanakun Wanita Semchuchot Wanita Semchuchot Prawporn Thaijongrak Prawporn Thaijongrak Prasert Sobhon Pei-San Tsai Rapeepun Vanichviriyakit Rapeepun Vanichviriyakit
author_sort	Phaivit Laphyai
title	Suppression of a Novel Vitellogenesis-Inhibiting Hormone Significantly Increases Ovarian Vitellogenesis in the Black Tiger Shrimp, Penaeus monodon
title_short	Suppression of a Novel Vitellogenesis-Inhibiting Hormone Significantly Increases Ovarian Vitellogenesis in the Black Tiger Shrimp, Penaeus monodon
title_full	Suppression of a Novel Vitellogenesis-Inhibiting Hormone Significantly Increases Ovarian Vitellogenesis in the Black Tiger Shrimp, Penaeus monodon
title_fullStr	Suppression of a Novel Vitellogenesis-Inhibiting Hormone Significantly Increases Ovarian Vitellogenesis in the Black Tiger Shrimp, Penaeus monodon
title_full_unstemmed	Suppression of a Novel Vitellogenesis-Inhibiting Hormone Significantly Increases Ovarian Vitellogenesis in the Black Tiger Shrimp, Penaeus monodon
title_sort	suppression of a novel vitellogenesis-inhibiting hormone significantly increases ovarian vitellogenesis in the black tiger shrimp, penaeus monodon
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/5012890398ac4ac1a63232ae0a763769
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Suppression of a Novel Vitellogenesis-Inhibiting Hormone Significantly Increases Ovarian Vitellogenesis in the Black Tiger Shrimp, Penaeus monodon

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