Sweat Gland Organoids Originating from Reprogrammed Epidermal Keratinocytes Functionally Recapitulated Damaged Skin

Abstract Restoration of sweat glands (SwGs) represents a great issue in patients with extensive skin defects. Recent methods combining organoid technology with cell fate reprogramming hold promise for developing new regenerative methods for SwG regeneration. Here, a practical strategy for engineerin...

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Autores principales:	Xiaoyan Sun, Jiangbing Xiang, Runkai Chen, Zhijun Geng, Lintao Wang, Yiqiong Liu, Shuaifei Ji, Huating Chen, Yan Li, Cuiping Zhang, Peng Liu, Tao Yue, Lei Dong, Xiaobing Fu
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	organoids regeneration reprogramming skin stem cells sweat glands Science Q
Acceso en línea:	https://doaj.org/article/501d69988c074e13960178787ef8a943
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spelling	oai:doaj.org-article:501d69988c074e13960178787ef8a9432021-11-17T08:40:31ZSweat Gland Organoids Originating from Reprogrammed Epidermal Keratinocytes Functionally Recapitulated Damaged Skin2198-384410.1002/advs.202103079https://doaj.org/article/501d69988c074e13960178787ef8a9432021-11-01T00:00:00Zhttps://doi.org/10.1002/advs.202103079https://doaj.org/toc/2198-3844Abstract Restoration of sweat glands (SwGs) represents a great issue in patients with extensive skin defects. Recent methods combining organoid technology with cell fate reprogramming hold promise for developing new regenerative methods for SwG regeneration. Here, a practical strategy for engineering functional human SwGs in vitro and in vivo is provided. First, by forced expression of the ectodysplasin‐A in human epidermal keratinocytes (HEKs) combined with specific SwG culture medium, HEKs are efficiently converted into SwG cells (iSwGCs). The iSwGCs show typical morphology, gene expression pattern, and functions resembling human primary SwG cells. Second, by culturing the iSwGCs in a special 3D culturing system, SwG organoids (iSwGOs) that exhibit structural and biological features characteristic of native SwGs are obtained. Finally, these iSwGOs are successfully transplanted into a mouse skin damage model and they develop into fully functioning SwGs in vivo. Regeneration of functional SwG organoids from reprogrammed HEKs highlights the great translational potential for personalized SwG regeneration in patients with large skin defects.Xiaoyan SunJiangbing XiangRunkai ChenZhijun GengLintao WangYiqiong LiuShuaifei JiHuating ChenYan LiCuiping ZhangPeng LiuTao YueLei DongXiaobing FuWileyarticleorganoidsregenerationreprogrammingskinstem cellssweat glandsScienceQENAdvanced Science, Vol 8, Iss 22, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	organoids regeneration reprogramming skin stem cells sweat glands Science Q
spellingShingle	organoids regeneration reprogramming skin stem cells sweat glands Science Q Xiaoyan Sun Jiangbing Xiang Runkai Chen Zhijun Geng Lintao Wang Yiqiong Liu Shuaifei Ji Huating Chen Yan Li Cuiping Zhang Peng Liu Tao Yue Lei Dong Xiaobing Fu Sweat Gland Organoids Originating from Reprogrammed Epidermal Keratinocytes Functionally Recapitulated Damaged Skin
description	Abstract Restoration of sweat glands (SwGs) represents a great issue in patients with extensive skin defects. Recent methods combining organoid technology with cell fate reprogramming hold promise for developing new regenerative methods for SwG regeneration. Here, a practical strategy for engineering functional human SwGs in vitro and in vivo is provided. First, by forced expression of the ectodysplasin‐A in human epidermal keratinocytes (HEKs) combined with specific SwG culture medium, HEKs are efficiently converted into SwG cells (iSwGCs). The iSwGCs show typical morphology, gene expression pattern, and functions resembling human primary SwG cells. Second, by culturing the iSwGCs in a special 3D culturing system, SwG organoids (iSwGOs) that exhibit structural and biological features characteristic of native SwGs are obtained. Finally, these iSwGOs are successfully transplanted into a mouse skin damage model and they develop into fully functioning SwGs in vivo. Regeneration of functional SwG organoids from reprogrammed HEKs highlights the great translational potential for personalized SwG regeneration in patients with large skin defects.
format	article
author	Xiaoyan Sun Jiangbing Xiang Runkai Chen Zhijun Geng Lintao Wang Yiqiong Liu Shuaifei Ji Huating Chen Yan Li Cuiping Zhang Peng Liu Tao Yue Lei Dong Xiaobing Fu
author_facet	Xiaoyan Sun Jiangbing Xiang Runkai Chen Zhijun Geng Lintao Wang Yiqiong Liu Shuaifei Ji Huating Chen Yan Li Cuiping Zhang Peng Liu Tao Yue Lei Dong Xiaobing Fu
author_sort	Xiaoyan Sun
title	Sweat Gland Organoids Originating from Reprogrammed Epidermal Keratinocytes Functionally Recapitulated Damaged Skin
title_short	Sweat Gland Organoids Originating from Reprogrammed Epidermal Keratinocytes Functionally Recapitulated Damaged Skin
title_full	Sweat Gland Organoids Originating from Reprogrammed Epidermal Keratinocytes Functionally Recapitulated Damaged Skin
title_fullStr	Sweat Gland Organoids Originating from Reprogrammed Epidermal Keratinocytes Functionally Recapitulated Damaged Skin
title_full_unstemmed	Sweat Gland Organoids Originating from Reprogrammed Epidermal Keratinocytes Functionally Recapitulated Damaged Skin
title_sort	sweat gland organoids originating from reprogrammed epidermal keratinocytes functionally recapitulated damaged skin
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/501d69988c074e13960178787ef8a943
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Sweat Gland Organoids Originating from Reprogrammed Epidermal Keratinocytes Functionally Recapitulated Damaged Skin

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