Synergistic Antimicrobial Effects of Silver/Transition-metal Combinatorial Treatments

Abstract Due to the emergence of multi-drug resistant strains, development of novel antibiotics has become a critical issue. One promising approach is the use of transition metals, since they exhibit rapid and significant toxicity, at low concentrations, in prokaryotic cells. Nevertheless, one main...

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Autores principales:	Javier A. Garza-Cervantes, Arturo Chávez-Reyes, Elena C. Castillo, Gerardo García-Rivas, Oscar Antonio Ortega-Rivera, Eva Salinas, Margarita Ortiz-Martínez, Sara Leticia Gómez-Flores, Jorge A. Peña-Martínez, Alan Pepi-Molina, Mario T. Treviño-González, Xristo Zarate, María Elena Cantú-Cárdenas, Carlos Enrique Escarcega-Gonzalez, J. Rubén Morones-Ramírez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/50210ea949b4482c82be7e858a277451
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spelling	oai:doaj.org-article:50210ea949b4482c82be7e858a2774512021-12-02T12:31:53ZSynergistic Antimicrobial Effects of Silver/Transition-metal Combinatorial Treatments10.1038/s41598-017-01017-72045-2322https://doaj.org/article/50210ea949b4482c82be7e858a2774512017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01017-7https://doaj.org/toc/2045-2322Abstract Due to the emergence of multi-drug resistant strains, development of novel antibiotics has become a critical issue. One promising approach is the use of transition metals, since they exhibit rapid and significant toxicity, at low concentrations, in prokaryotic cells. Nevertheless, one main drawback of transition metals is their toxicity in eukaryotic cells. Here, we show that the barriers to use them as therapeutic agents could be mitigated by combining them with silver. We demonstrate that synergism of combinatorial treatments (Silver/transition metals, including Zn, Co, Cd, Ni, and Cu) increases up to 8-fold their antimicrobial effect, when compared to their individual effects, against E. coli and B. subtilis. We find that most combinatorial treatments exhibit synergistic antimicrobial effects at low/non-toxic concentrations to human keratinocyte cells, blast and melanoma rat cell lines. Moreover, we show that silver/(Cu, Ni, and Zn) increase prokaryotic cell permeability at sub-inhibitory concentrations, demonstrating this to be a possible mechanism of the synergistic behavior. Together, these results suggest that these combinatorial treatments will play an important role in the future development of antimicrobial agents and treatments against infections. In specific, the cytotoxicity experiments show that the combinations have great potential in the treatment of topical infections.Javier A. Garza-CervantesArturo Chávez-ReyesElena C. CastilloGerardo García-RivasOscar Antonio Ortega-RiveraEva SalinasMargarita Ortiz-MartínezSara Leticia Gómez-FloresJorge A. Peña-MartínezAlan Pepi-MolinaMario T. Treviño-GonzálezXristo ZarateMaría Elena Cantú-CárdenasCarlos Enrique Escarcega-GonzalezJ. Rubén Morones-RamírezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Javier A. Garza-Cervantes Arturo Chávez-Reyes Elena C. Castillo Gerardo García-Rivas Oscar Antonio Ortega-Rivera Eva Salinas Margarita Ortiz-Martínez Sara Leticia Gómez-Flores Jorge A. Peña-Martínez Alan Pepi-Molina Mario T. Treviño-González Xristo Zarate María Elena Cantú-Cárdenas Carlos Enrique Escarcega-Gonzalez J. Rubén Morones-Ramírez Synergistic Antimicrobial Effects of Silver/Transition-metal Combinatorial Treatments
description	Abstract Due to the emergence of multi-drug resistant strains, development of novel antibiotics has become a critical issue. One promising approach is the use of transition metals, since they exhibit rapid and significant toxicity, at low concentrations, in prokaryotic cells. Nevertheless, one main drawback of transition metals is their toxicity in eukaryotic cells. Here, we show that the barriers to use them as therapeutic agents could be mitigated by combining them with silver. We demonstrate that synergism of combinatorial treatments (Silver/transition metals, including Zn, Co, Cd, Ni, and Cu) increases up to 8-fold their antimicrobial effect, when compared to their individual effects, against E. coli and B. subtilis. We find that most combinatorial treatments exhibit synergistic antimicrobial effects at low/non-toxic concentrations to human keratinocyte cells, blast and melanoma rat cell lines. Moreover, we show that silver/(Cu, Ni, and Zn) increase prokaryotic cell permeability at sub-inhibitory concentrations, demonstrating this to be a possible mechanism of the synergistic behavior. Together, these results suggest that these combinatorial treatments will play an important role in the future development of antimicrobial agents and treatments against infections. In specific, the cytotoxicity experiments show that the combinations have great potential in the treatment of topical infections.
format	article
author	Javier A. Garza-Cervantes Arturo Chávez-Reyes Elena C. Castillo Gerardo García-Rivas Oscar Antonio Ortega-Rivera Eva Salinas Margarita Ortiz-Martínez Sara Leticia Gómez-Flores Jorge A. Peña-Martínez Alan Pepi-Molina Mario T. Treviño-González Xristo Zarate María Elena Cantú-Cárdenas Carlos Enrique Escarcega-Gonzalez J. Rubén Morones-Ramírez
author_facet	Javier A. Garza-Cervantes Arturo Chávez-Reyes Elena C. Castillo Gerardo García-Rivas Oscar Antonio Ortega-Rivera Eva Salinas Margarita Ortiz-Martínez Sara Leticia Gómez-Flores Jorge A. Peña-Martínez Alan Pepi-Molina Mario T. Treviño-González Xristo Zarate María Elena Cantú-Cárdenas Carlos Enrique Escarcega-Gonzalez J. Rubén Morones-Ramírez
author_sort	Javier A. Garza-Cervantes
title	Synergistic Antimicrobial Effects of Silver/Transition-metal Combinatorial Treatments
title_short	Synergistic Antimicrobial Effects of Silver/Transition-metal Combinatorial Treatments
title_full	Synergistic Antimicrobial Effects of Silver/Transition-metal Combinatorial Treatments
title_fullStr	Synergistic Antimicrobial Effects of Silver/Transition-metal Combinatorial Treatments
title_full_unstemmed	Synergistic Antimicrobial Effects of Silver/Transition-metal Combinatorial Treatments
title_sort	synergistic antimicrobial effects of silver/transition-metal combinatorial treatments
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/50210ea949b4482c82be7e858a277451
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Synergistic Antimicrobial Effects of Silver/Transition-metal Combinatorial Treatments

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