SGBS cells as a model of human adipocyte browning: A comprehensive comparative study with primary human white subcutaneous adipocytes

Abstract The Simpson Golabi Behmel Syndrome (SGBS) pre-adipocyte cell strain is widely considered to be a representative in vitro model of human white pre-adipocytes. A recent study suggested that SGBS adipocytes exhibit an unexpected transient brown phenotype. Here, we comprehensively examined key...

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Autores principales:	Chia Rou Yeo, Madhur Agrawal, Shawn Hoon, Asim Shabbir, Manu Kunaal Shrivastava, Shiqi Huang, Chin Meng Khoo, Vanna Chhay, M. Shabeer Yassin, E. Shyong Tai, Antonio Vidal-Puig, Sue-Anne Toh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/502735b3ae004a90b34ceaaa49ad3071
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spelling	oai:doaj.org-article:502735b3ae004a90b34ceaaa49ad30712021-12-02T12:32:25ZSGBS cells as a model of human adipocyte browning: A comprehensive comparative study with primary human white subcutaneous adipocytes10.1038/s41598-017-04369-22045-2322https://doaj.org/article/502735b3ae004a90b34ceaaa49ad30712017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04369-2https://doaj.org/toc/2045-2322Abstract The Simpson Golabi Behmel Syndrome (SGBS) pre-adipocyte cell strain is widely considered to be a representative in vitro model of human white pre-adipocytes. A recent study suggested that SGBS adipocytes exhibit an unexpected transient brown phenotype. Here, we comprehensively examined key differences between SGBS adipocytes and primary human white subcutaneous (PHWSC) adipocytes. RNA-Seq analysis revealed that extracellular matrix (ECM)-receptor interaction and metabolic pathways were the top two KEGG pathways significantly enriched in SGBS adipocytes, which included positively enriched mitochondrial respiration and oxidation pathways. Compared to PHWSC adipocytes, SGBS adipocytes showed not only greater induction of adipogenic gene expression during differentiation but also increased levels of UCP1 mRNA and protein expression. Functionally, SGBS adipocytes displayed higher ISO-induced basal leak respiration and overall oxygen consumption rate, along with increased triglyceride accumulation and insulin-stimulated glucose uptake. In conclusion, we confirmed that SGBS adipocytes, which are considered of white adipose tissue origin can shift towards a brown/beige adipocyte phenotype. These differences indicate SGBS cells may help to identify mechanisms leading to browning, and inform our understanding for the use of SGBS vis-à-vis primary human subcutaneous adipocytes as a human white adipocyte model, guiding the selection of appropriate cell models in future metabolic research.Chia Rou YeoMadhur AgrawalShawn HoonAsim ShabbirManu Kunaal ShrivastavaShiqi HuangChin Meng KhooVanna ChhayM. Shabeer YassinE. Shyong TaiAntonio Vidal-PuigSue-Anne TohNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chia Rou Yeo Madhur Agrawal Shawn Hoon Asim Shabbir Manu Kunaal Shrivastava Shiqi Huang Chin Meng Khoo Vanna Chhay M. Shabeer Yassin E. Shyong Tai Antonio Vidal-Puig Sue-Anne Toh SGBS cells as a model of human adipocyte browning: A comprehensive comparative study with primary human white subcutaneous adipocytes
description	Abstract The Simpson Golabi Behmel Syndrome (SGBS) pre-adipocyte cell strain is widely considered to be a representative in vitro model of human white pre-adipocytes. A recent study suggested that SGBS adipocytes exhibit an unexpected transient brown phenotype. Here, we comprehensively examined key differences between SGBS adipocytes and primary human white subcutaneous (PHWSC) adipocytes. RNA-Seq analysis revealed that extracellular matrix (ECM)-receptor interaction and metabolic pathways were the top two KEGG pathways significantly enriched in SGBS adipocytes, which included positively enriched mitochondrial respiration and oxidation pathways. Compared to PHWSC adipocytes, SGBS adipocytes showed not only greater induction of adipogenic gene expression during differentiation but also increased levels of UCP1 mRNA and protein expression. Functionally, SGBS adipocytes displayed higher ISO-induced basal leak respiration and overall oxygen consumption rate, along with increased triglyceride accumulation and insulin-stimulated glucose uptake. In conclusion, we confirmed that SGBS adipocytes, which are considered of white adipose tissue origin can shift towards a brown/beige adipocyte phenotype. These differences indicate SGBS cells may help to identify mechanisms leading to browning, and inform our understanding for the use of SGBS vis-à-vis primary human subcutaneous adipocytes as a human white adipocyte model, guiding the selection of appropriate cell models in future metabolic research.
format	article
author	Chia Rou Yeo Madhur Agrawal Shawn Hoon Asim Shabbir Manu Kunaal Shrivastava Shiqi Huang Chin Meng Khoo Vanna Chhay M. Shabeer Yassin E. Shyong Tai Antonio Vidal-Puig Sue-Anne Toh
author_facet	Chia Rou Yeo Madhur Agrawal Shawn Hoon Asim Shabbir Manu Kunaal Shrivastava Shiqi Huang Chin Meng Khoo Vanna Chhay M. Shabeer Yassin E. Shyong Tai Antonio Vidal-Puig Sue-Anne Toh
author_sort	Chia Rou Yeo
title	SGBS cells as a model of human adipocyte browning: A comprehensive comparative study with primary human white subcutaneous adipocytes
title_short	SGBS cells as a model of human adipocyte browning: A comprehensive comparative study with primary human white subcutaneous adipocytes
title_full	SGBS cells as a model of human adipocyte browning: A comprehensive comparative study with primary human white subcutaneous adipocytes
title_fullStr	SGBS cells as a model of human adipocyte browning: A comprehensive comparative study with primary human white subcutaneous adipocytes
title_full_unstemmed	SGBS cells as a model of human adipocyte browning: A comprehensive comparative study with primary human white subcutaneous adipocytes
title_sort	sgbs cells as a model of human adipocyte browning: a comprehensive comparative study with primary human white subcutaneous adipocytes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/502735b3ae004a90b34ceaaa49ad3071
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SGBS cells as a model of human adipocyte browning: A comprehensive comparative study with primary human white subcutaneous adipocytes

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