A live-imaging protocol to track cell movement in the Xenopus embryo

Summary: Tracking individual cell movement during development is challenging, particularly in tissues subjected to major remodeling. Currently, most live imaging techniques in Xenopus are limited to tissue explants and/or to superficial cells. We describe here a protocol to track immature multicilia...

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Autores principales:	Alexandre Chuyen, Fabrice Daian, Andrea Pasini, Laurent Kodjabachian
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell Biology Developmental biology Microscopy Model Organisms Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/502d4d7a2c6e4366ba618e1de906eded
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spelling	oai:doaj.org-article:502d4d7a2c6e4366ba618e1de906eded2021-11-04T04:39:49ZA live-imaging protocol to track cell movement in the Xenopus embryo2666-166710.1016/j.xpro.2021.100928https://doaj.org/article/502d4d7a2c6e4366ba618e1de906eded2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006341https://doaj.org/toc/2666-1667Summary: Tracking individual cell movement during development is challenging, particularly in tissues subjected to major remodeling. Currently, most live imaging techniques in Xenopus are limited to tissue explants and/or to superficial cells. We describe here a protocol to track immature multiciliated cells (MCCs) moving within the inner epidermal layer of a whole embryo. In addition, we present a data processing protocol to uncouple the movements of individual cells from the coplanar drifts of the tissue in which they are embedded.For complete details on the use and execution of this protocol, please refer to Chuyen et al. (2021).Alexandre ChuyenFabrice DaianAndrea PasiniLaurent KodjabachianElsevierarticleCell BiologyDevelopmental biologyMicroscopyModel OrganismsScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100928- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell Biology Developmental biology Microscopy Model Organisms Science (General) Q1-390
spellingShingle	Cell Biology Developmental biology Microscopy Model Organisms Science (General) Q1-390 Alexandre Chuyen Fabrice Daian Andrea Pasini Laurent Kodjabachian A live-imaging protocol to track cell movement in the Xenopus embryo
description	Summary: Tracking individual cell movement during development is challenging, particularly in tissues subjected to major remodeling. Currently, most live imaging techniques in Xenopus are limited to tissue explants and/or to superficial cells. We describe here a protocol to track immature multiciliated cells (MCCs) moving within the inner epidermal layer of a whole embryo. In addition, we present a data processing protocol to uncouple the movements of individual cells from the coplanar drifts of the tissue in which they are embedded.For complete details on the use and execution of this protocol, please refer to Chuyen et al. (2021).
format	article
author	Alexandre Chuyen Fabrice Daian Andrea Pasini Laurent Kodjabachian
author_facet	Alexandre Chuyen Fabrice Daian Andrea Pasini Laurent Kodjabachian
author_sort	Alexandre Chuyen
title	A live-imaging protocol to track cell movement in the Xenopus embryo
title_short	A live-imaging protocol to track cell movement in the Xenopus embryo
title_full	A live-imaging protocol to track cell movement in the Xenopus embryo
title_fullStr	A live-imaging protocol to track cell movement in the Xenopus embryo
title_full_unstemmed	A live-imaging protocol to track cell movement in the Xenopus embryo
title_sort	live-imaging protocol to track cell movement in the xenopus embryo
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/502d4d7a2c6e4366ba618e1de906eded
work_keys_str_mv	AT alexandrechuyen aliveimagingprotocoltotrackcellmovementinthexenopusembryo AT fabricedaian aliveimagingprotocoltotrackcellmovementinthexenopusembryo AT andreapasini aliveimagingprotocoltotrackcellmovementinthexenopusembryo AT laurentkodjabachian aliveimagingprotocoltotrackcellmovementinthexenopusembryo AT alexandrechuyen liveimagingprotocoltotrackcellmovementinthexenopusembryo AT fabricedaian liveimagingprotocoltotrackcellmovementinthexenopusembryo AT andreapasini liveimagingprotocoltotrackcellmovementinthexenopusembryo AT laurentkodjabachian liveimagingprotocoltotrackcellmovementinthexenopusembryo
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A live-imaging protocol to track cell movement in the Xenopus embryo

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