Knockdown of long non-coding RNA H19 inhibits multiple myeloma cell growth via NF-κB pathway

Abstract Long non-coding RNAs (lncRNAs) are implicated in the complex network of cancer including Multiple myeloma (MM) and play important roles in tumor development. lncH19 was significantly up-regulated in multiple cancer types, suggesting it is a potential oncogene. However, the exact functions a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yuanyuan Sun, Jing Pan, Ning Zhang, Wei Wei, Shanshan Yu, Limei Ai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5038113b6ae14c88a3b31a48e16b698d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5038113b6ae14c88a3b31a48e16b698d
record_format	dspace
spelling	oai:doaj.org-article:5038113b6ae14c88a3b31a48e16b698d2021-12-02T15:05:39ZKnockdown of long non-coding RNA H19 inhibits multiple myeloma cell growth via NF-κB pathway10.1038/s41598-017-18056-92045-2322https://doaj.org/article/5038113b6ae14c88a3b31a48e16b698d2017-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-18056-9https://doaj.org/toc/2045-2322Abstract Long non-coding RNAs (lncRNAs) are implicated in the complex network of cancer including Multiple myeloma (MM) and play important roles in tumor development. lncH19 was significantly up-regulated in multiple cancer types, suggesting it is a potential oncogene. However, the exact functions and downstream mechanisms are largely unknown. This study aimed to investigate whether H19 participates in the cell growth of MM and elucidate the underlying mechanism. We found that H19 was abnormally overexpressed in MM cell lines and sorted CD138+ MM bone marrow tissues. H19 knockdown induced by shRNA transfection significantly inhibited proliferation, viability and colony formation in MM cells, as well as inactivated NF-κB pathway. Moreover, combination treatment of H19 knockdown and NF-κB suppression (induced by specific inhibitor PDTC) produced synergistically inhibitory effects. Bone marrow expression of H19 was positively associated with circulating IL-6 or IL-8 level in the same MM patients. And patients with high expression of H19 had a lower survival rate. Taken together, we confirmed the abnormal upregulation of a novel lncRNA, H19, in human MM. H19 was involved in MM cell growth. The linkage between H19 and NF-κB pathway may provide a novel interpretation for the mechanism of H19’s growth regulation in MM.Yuanyuan SunJing PanNing ZhangWei WeiShanshan YuLimei AiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuanyuan Sun Jing Pan Ning Zhang Wei Wei Shanshan Yu Limei Ai Knockdown of long non-coding RNA H19 inhibits multiple myeloma cell growth via NF-κB pathway
description	Abstract Long non-coding RNAs (lncRNAs) are implicated in the complex network of cancer including Multiple myeloma (MM) and play important roles in tumor development. lncH19 was significantly up-regulated in multiple cancer types, suggesting it is a potential oncogene. However, the exact functions and downstream mechanisms are largely unknown. This study aimed to investigate whether H19 participates in the cell growth of MM and elucidate the underlying mechanism. We found that H19 was abnormally overexpressed in MM cell lines and sorted CD138+ MM bone marrow tissues. H19 knockdown induced by shRNA transfection significantly inhibited proliferation, viability and colony formation in MM cells, as well as inactivated NF-κB pathway. Moreover, combination treatment of H19 knockdown and NF-κB suppression (induced by specific inhibitor PDTC) produced synergistically inhibitory effects. Bone marrow expression of H19 was positively associated with circulating IL-6 or IL-8 level in the same MM patients. And patients with high expression of H19 had a lower survival rate. Taken together, we confirmed the abnormal upregulation of a novel lncRNA, H19, in human MM. H19 was involved in MM cell growth. The linkage between H19 and NF-κB pathway may provide a novel interpretation for the mechanism of H19’s growth regulation in MM.
format	article
author	Yuanyuan Sun Jing Pan Ning Zhang Wei Wei Shanshan Yu Limei Ai
author_facet	Yuanyuan Sun Jing Pan Ning Zhang Wei Wei Shanshan Yu Limei Ai
author_sort	Yuanyuan Sun
title	Knockdown of long non-coding RNA H19 inhibits multiple myeloma cell growth via NF-κB pathway
title_short	Knockdown of long non-coding RNA H19 inhibits multiple myeloma cell growth via NF-κB pathway
title_full	Knockdown of long non-coding RNA H19 inhibits multiple myeloma cell growth via NF-κB pathway
title_fullStr	Knockdown of long non-coding RNA H19 inhibits multiple myeloma cell growth via NF-κB pathway
title_full_unstemmed	Knockdown of long non-coding RNA H19 inhibits multiple myeloma cell growth via NF-κB pathway
title_sort	knockdown of long non-coding rna h19 inhibits multiple myeloma cell growth via nf-κb pathway
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5038113b6ae14c88a3b31a48e16b698d
work_keys_str_mv	AT yuanyuansun knockdownoflongnoncodingrnah19inhibitsmultiplemyelomacellgrowthvianfkbpathway AT jingpan knockdownoflongnoncodingrnah19inhibitsmultiplemyelomacellgrowthvianfkbpathway AT ningzhang knockdownoflongnoncodingrnah19inhibitsmultiplemyelomacellgrowthvianfkbpathway AT weiwei knockdownoflongnoncodingrnah19inhibitsmultiplemyelomacellgrowthvianfkbpathway AT shanshanyu knockdownoflongnoncodingrnah19inhibitsmultiplemyelomacellgrowthvianfkbpathway AT limeiai knockdownoflongnoncodingrnah19inhibitsmultiplemyelomacellgrowthvianfkbpathway
_version_	1718388763885830144

Knockdown of long non-coding RNA H19 inhibits multiple myeloma cell growth via NF-κB pathway

Ejemplares similares