Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane

Chorioallantoic membrane (CAM) is responsible for respiratory gas exchange, eggshell calcium transport, embryonic acid-base equilibrium, allantoic ion, and water reabsorption during avian embryonic development. To further understand the timing of CAM gene expression during chick embryonic developmen...

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Autores principales:	Qun Huang, Ran Yang, Qia Wang, Hui Teng, Hongbo Song, Fang Geng, Peng Luo
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	Incubation Chorioallantoic membrane Transcriptome Calcium transport Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/503adf1b3ef2425896c87e42aed58f64
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spelling	oai:doaj.org-article:503adf1b3ef2425896c87e42aed58f642021-11-28T04:32:11ZTranscriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane2213-453010.1016/j.fshw.2021.11.018https://doaj.org/article/503adf1b3ef2425896c87e42aed58f642022-03-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2213453021001142https://doaj.org/toc/2213-4530Chorioallantoic membrane (CAM) is responsible for respiratory gas exchange, eggshell calcium transport, embryonic acid-base equilibrium, allantoic ion, and water reabsorption during avian embryonic development. To further understand the timing of CAM gene expression during chick embryonic development, especially the calcium absorption mechanism, transcriptome quantitative comparative analysis was conducted on chick CAM during the embryonic period (E) of 9, 13, 17, and 20 days. A total of 6378 differentially expressed genes (DEGs) were identified. Functional enrichment analysis of DEGs showed that CAM DEGs were mainly involved in biological processes such as “ion transport regulation”, “immune response” and “cell cycle”. Time series analysis of the differential genes showed that the functional cells of CAM began to proliferate and differentiate at E9 and the calcium content of egg embryo increased significantly at E13. Simultaneously, the observation of the ultrastructure of the eggshell showed that the interstice of the fiber layer was enlarged at E13, and the mastoid layer was partly exposed. Therefore, it is preliminarily inferred that CAM calcium transport starts at E13, and genes such as TRPV6, S100A10, and RANKL cooperate to regulate calcium release and transport.Qun HuangRan YangQia WangHui TengHongbo SongFang GengPeng LuoKeAi Communications Co., Ltd.articleIncubationChorioallantoic membraneTranscriptomeCalcium transportNutrition. Foods and food supplyTX341-641ENFood Science and Human Wellness, Vol 11, Iss 2, Pp 383-392 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Incubation Chorioallantoic membrane Transcriptome Calcium transport Nutrition. Foods and food supply TX341-641
spellingShingle	Incubation Chorioallantoic membrane Transcriptome Calcium transport Nutrition. Foods and food supply TX341-641 Qun Huang Ran Yang Qia Wang Hui Teng Hongbo Song Fang Geng Peng Luo Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane
description	Chorioallantoic membrane (CAM) is responsible for respiratory gas exchange, eggshell calcium transport, embryonic acid-base equilibrium, allantoic ion, and water reabsorption during avian embryonic development. To further understand the timing of CAM gene expression during chick embryonic development, especially the calcium absorption mechanism, transcriptome quantitative comparative analysis was conducted on chick CAM during the embryonic period (E) of 9, 13, 17, and 20 days. A total of 6378 differentially expressed genes (DEGs) were identified. Functional enrichment analysis of DEGs showed that CAM DEGs were mainly involved in biological processes such as “ion transport regulation”, “immune response” and “cell cycle”. Time series analysis of the differential genes showed that the functional cells of CAM began to proliferate and differentiate at E9 and the calcium content of egg embryo increased significantly at E13. Simultaneously, the observation of the ultrastructure of the eggshell showed that the interstice of the fiber layer was enlarged at E13, and the mastoid layer was partly exposed. Therefore, it is preliminarily inferred that CAM calcium transport starts at E13, and genes such as TRPV6, S100A10, and RANKL cooperate to regulate calcium release and transport.
format	article
author	Qun Huang Ran Yang Qia Wang Hui Teng Hongbo Song Fang Geng Peng Luo
author_facet	Qun Huang Ran Yang Qia Wang Hui Teng Hongbo Song Fang Geng Peng Luo
author_sort	Qun Huang
title	Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane
title_short	Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane
title_full	Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane
title_fullStr	Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane
title_full_unstemmed	Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane
title_sort	transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/503adf1b3ef2425896c87e42aed58f64
work_keys_str_mv	AT qunhuang transcriptomebasedinsightsintothecalciumtransportmechanismofchickchorioallantoicmembrane AT ranyang transcriptomebasedinsightsintothecalciumtransportmechanismofchickchorioallantoicmembrane AT qiawang transcriptomebasedinsightsintothecalciumtransportmechanismofchickchorioallantoicmembrane AT huiteng transcriptomebasedinsightsintothecalciumtransportmechanismofchickchorioallantoicmembrane AT hongbosong transcriptomebasedinsightsintothecalciumtransportmechanismofchickchorioallantoicmembrane AT fanggeng transcriptomebasedinsightsintothecalciumtransportmechanismofchickchorioallantoicmembrane AT pengluo transcriptomebasedinsightsintothecalciumtransportmechanismofchickchorioallantoicmembrane
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Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane

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