The effects of Omarigliptin on promoting osteoblastic differentiation

Osteoporosis significantly impacts the normal life of the elderly and is reported to be closely related to dysfunction of osteoblastic differentiation. Runt-related transcription factor-2 (Runx2) is a critical transcriptional factor involved in the regulation of osteoblast differentiation. Omariglip...

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Autores principales:	Fake Liao, Xiunian Hu, Rijiang Chen
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	omarigliptin osteoporosis osteoblastic differentiation runx2 Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/504331a7a6ad4b2cbadcca6120c029fd
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spelling	oai:doaj.org-article:504331a7a6ad4b2cbadcca6120c029fd2021-11-26T11:19:49ZThe effects of Omarigliptin on promoting osteoblastic differentiation2165-59792165-598710.1080/21655979.2021.1999366https://doaj.org/article/504331a7a6ad4b2cbadcca6120c029fd2021-10-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1999366https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Osteoporosis significantly impacts the normal life of the elderly and is reported to be closely related to dysfunction of osteoblastic differentiation. Runt-related transcription factor-2 (Runx2) is a critical transcriptional factor involved in the regulation of osteoblast differentiation. Omarigliptin is a novel dipeptidyl peptidase-4 (DDP-4) inhibitor and this study proposes to probe into its possible therapeutic function against Osteoporosis by investigating its impacts on osteoblastic differentiation. Osteogenic medium was used to induce osteoblastic differentiation in MC3T3‑E1 cells, and was verified by the increased alkaline phosphatase (ALP) activity, enhanced mineralization, and promoted expression level of osteoblastic differentiation-related factors, including bone morphogenetic protein-2 (BMP-2), ALP, osteocalcin (Ocn), collagen type I alpha 1 (Col1a1), Collagen Type I alpha 2 (Col1a2), Runx2, osterix (Sp7), fibroblast growth factor receptor 2 (Fgfr2), and fibroblast growth factor receptor 3 (Fgfr3), accompanied by the activation of the p38 and Akt pathways. After treatment with Omarigliptin, the ALP activity and mineralization were further promoted, accompanied by the further upregulation of osteoblastic differentiation-related factors, and activation of the p38 and Akt pathways. Lastly, Omarigliptin-induced osteoblastic differentiation, promoted ALP activity, and increased expression levels of Sp7, Fgfr2, Fgfr3, BMP-2, Ocn, ALP, Col1a1, and Col1a2, in the osteogenic medium- cultured MC3T3‑E1 cells were dramatically abolished by the knockdown of Runx2. Taken together, our data reveal that Omarigliptin promoted osteoblastic differentiation by regulating Runx2.Fake LiaoXiunian HuRijiang ChenTaylor & Francis Grouparticleomarigliptinosteoporosisosteoblastic differentiationrunx2BiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	omarigliptin osteoporosis osteoblastic differentiation runx2 Biotechnology TP248.13-248.65
spellingShingle	omarigliptin osteoporosis osteoblastic differentiation runx2 Biotechnology TP248.13-248.65 Fake Liao Xiunian Hu Rijiang Chen The effects of Omarigliptin on promoting osteoblastic differentiation
description	Osteoporosis significantly impacts the normal life of the elderly and is reported to be closely related to dysfunction of osteoblastic differentiation. Runt-related transcription factor-2 (Runx2) is a critical transcriptional factor involved in the regulation of osteoblast differentiation. Omarigliptin is a novel dipeptidyl peptidase-4 (DDP-4) inhibitor and this study proposes to probe into its possible therapeutic function against Osteoporosis by investigating its impacts on osteoblastic differentiation. Osteogenic medium was used to induce osteoblastic differentiation in MC3T3‑E1 cells, and was verified by the increased alkaline phosphatase (ALP) activity, enhanced mineralization, and promoted expression level of osteoblastic differentiation-related factors, including bone morphogenetic protein-2 (BMP-2), ALP, osteocalcin (Ocn), collagen type I alpha 1 (Col1a1), Collagen Type I alpha 2 (Col1a2), Runx2, osterix (Sp7), fibroblast growth factor receptor 2 (Fgfr2), and fibroblast growth factor receptor 3 (Fgfr3), accompanied by the activation of the p38 and Akt pathways. After treatment with Omarigliptin, the ALP activity and mineralization were further promoted, accompanied by the further upregulation of osteoblastic differentiation-related factors, and activation of the p38 and Akt pathways. Lastly, Omarigliptin-induced osteoblastic differentiation, promoted ALP activity, and increased expression levels of Sp7, Fgfr2, Fgfr3, BMP-2, Ocn, ALP, Col1a1, and Col1a2, in the osteogenic medium- cultured MC3T3‑E1 cells were dramatically abolished by the knockdown of Runx2. Taken together, our data reveal that Omarigliptin promoted osteoblastic differentiation by regulating Runx2.
format	article
author	Fake Liao Xiunian Hu Rijiang Chen
author_facet	Fake Liao Xiunian Hu Rijiang Chen
author_sort	Fake Liao
title	The effects of Omarigliptin on promoting osteoblastic differentiation
title_short	The effects of Omarigliptin on promoting osteoblastic differentiation
title_full	The effects of Omarigliptin on promoting osteoblastic differentiation
title_fullStr	The effects of Omarigliptin on promoting osteoblastic differentiation
title_full_unstemmed	The effects of Omarigliptin on promoting osteoblastic differentiation
title_sort	effects of omarigliptin on promoting osteoblastic differentiation
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/504331a7a6ad4b2cbadcca6120c029fd
work_keys_str_mv	AT fakeliao theeffectsofomarigliptinonpromotingosteoblasticdifferentiation AT xiunianhu theeffectsofomarigliptinonpromotingosteoblasticdifferentiation AT rijiangchen theeffectsofomarigliptinonpromotingosteoblasticdifferentiation AT fakeliao effectsofomarigliptinonpromotingosteoblasticdifferentiation AT xiunianhu effectsofomarigliptinonpromotingosteoblasticdifferentiation AT rijiangchen effectsofomarigliptinonpromotingosteoblasticdifferentiation
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The effects of Omarigliptin on promoting osteoblastic differentiation

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